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GC-MS法分析透骨香根和茎叶中挥发性成分的差异 Δ

蒲 健 ，晏 婷 ，蒯晴叶 ，王爱民 ，郑 林 ，李勇军 ，黄 勇 （1.贵州益佰制药股份有限公司，贵阳
1＊
3 #
3
3
4
2，3
2，3
550008；2.贵州医科大学药学院，贵阳 550004；3.贵州医科大学贵州省药物制剂重点实验室/药用植物功效与
利用国家重点实验室，贵阳 550004；4.贵州医科大学民族药与中药开发应用教育部工程研究中心，贵阳
550004）
中图分类号 R282 文献标志码 A 文章编号 1001-0408（2020）14-1695-05
DOI 10.6039/j.issn.1001-0408.2020.14.06

摘 要 目的：比较透骨香根和茎叶中挥发性成分的差异。方法：采用水蒸气蒸馏法提取透骨香根和茎叶的挥发油，并用气质联
用技术对其化学成分进行分析，使用美国国家标准与技术局2011标准质谱图库进行检索，选择匹配度高于80的色谱峰，结合相关
文献进行鉴定，并通过峰面积归一化法计算各化学成分的相对百分含量。结果：从透骨香根挥发油中分析检测出95个色谱峰，鉴
定出54种化学成分，占根挥发性成分总含量的82.35％，其中相对含量较高的成分有水杨酸甲酯（20.30％）、棕榈酸（19.86％）、亚
油酸（9.26％）、菲（4.37％）等；从透骨香茎叶挥发油中分析检测出69个色谱峰，鉴定出46种化学成分，占茎叶挥发性成分总含量的
97.10％，其中相对百分含量较高的成分有水杨酸甲酯（86.72％）、棕榈酸（2.60％）、亚麻酸（1.25％）等。两者均含有烯烃类、酯类、
酸类等共有成分16个。结论：透骨香根和茎叶中挥发性成分主要为酯类和酸类，二者成分种类有一定的相似性，但根中酸类成分
含量较高、茎叶中酯类成分含量较高。
关键词 透骨香；根；茎叶；挥发性成分；水蒸气蒸馏法；气质联用技术

Difference Analysis of Volatile Components in Roots，Stems and Leaves of Gaultheria yunnanensis by
GC-MS
PU Jian ，YAN Ting ，KUAI Qingye ，WANG Aimin ，ZHENG Lin ，LI Yongjun ，HUANG Yong（1. Guizhou
4
3
3
3
1
2，3
2，3
Yibai Pharmaceutical Co.，Ltd.，Guiyang 550008，China；2. School of Pharmacy，Guizhou Medical University，
Guiyang 550004，China；3. Guizhou Provincial Key Laboratory of Pharmaceutical Preparations/State Key
Laboratory of Medicinal Plant Efficacy and Utilization，Guizhou Medical University，Guiyang 550004，China；
4. Education Ministry Engineering Research Center for the Development and Application of Ethnic Medicine and
TCM，Guizhou Medical University，Guiyang 550004，China）
ABSTRACT OBJECTIVE：To compare the volatile components extracted from the roots，stems and leaves of Gaultheria
yunnanensis. METHODS：Steam distillation method was used to extract the volatile oils from roots，stems and leaves of G.
yunnanensis. Chemical constituents were analyzed by GC-MS. NIST 2011 standard mass spectral library was adopted to select the
chromatographic peak with a matching degree higher than 80，and combined with relevant literatures for identification. Relative
percentages of chemical constituents were calculated by peak area normalization. RESULTS：Totally 95 chromatographic peaks
were detected in valatile oil from the roots of G. yunnanensis and 54 chemical constituents were identified，accounting for 82.35％
of the total content of root volatile components. The constituents with relatively high content were methyl salicylate（20.30％），
n-hexadecanoic acid （19.86％），（Z，Z）-9，12-octadecadienoic acid （9.26％），phenanthrene （4.37％） and so on. Totally 69
chromatographic peaks were detected in volatile oil from the stems and leaves of G. yunnanensis and 46 chemical constituents were
identified，accounting for 97.10％ of the total content of volatile components in stems and leaves. The constituents with relatively
high content were methyl salicylate（86.72％），n-hexadecanoic acid（2.60％），（Z，Z，Z）-9，12，15-octadecatrienoic acid（1.25％）
and so on. Both of them contained 16 common constituents
Δ 基金项目：国家重点研发计划项目（No.2018YFC1708100）；国
such as alkenes esters，acids and so on. CONCLUSIONS：The
家自然科学基金资助项目（No.81960763，No.U1812403）；贵州省科技
chemical constituents of volatile oils extracted from the roots，
计划项目（No.黔科合平台人才〔2019〕5407，No.黔科合平台人才
〔2019〕5660，No.黔科合平台人才〔2017〕5601）；中央引导地方科技发 stems and leaves of G. yunnanensis are mainly esters and
展专项资金项目（No.黔科中引地〔2018〕4006）；贵阳市科技计划项目 acids. The components are similar to each other， but the
（No.筑科合同〔2017〕30-29号） contents of acids in the roots and esters in the leaves and
＊药师。研究方向：中药质量控制及新药研发。电话：0851- stems are higher.
86908468。E-mail：1037930332@qq.com KEYWORDS Gaultheria yunnanensis； Roots； Stems and
# 通信作者：教授，博士。研究方向：中药药效物质基础及新药研 leaves；Volatile components；Steam distillation；GC-MS
发。电话：0851-86908468。E-mail：mailofhy@126.com

中国药房 2020年第31卷第14期 China Pharmacy 2020 Vol. 31 No. 14 ·1695 ·