Page 45 - 202004

不同产地佛手的挥发油成分比较分析 Δ

1
1 #
2
1＊
赵永艳 ，张军银 ，彭 腾 ，何沛煜 ，匡 宇（1.成都中医药大学药学院/西南特色中药资源国家重点实验室/
1
教育部中药材标准化重点实验室/中药资源系统研究与开发利用省部共建国家重点实验室培育基地，成都
611137；2.遂宁市中医院药剂科，四川 遂宁 629000）
中图分类号 R284 文献标志码 A 文章编号 1001-0408（2020）04-0423-06
DOI 10.6039/j.issn.1001-0408.2020.04.07

摘 要 目的：比较不同产地佛手挥发油成分的差异。方法：采用水蒸气蒸馏法提取10个不同产地佛手挥发油并计算得率；采用
气质联用法分析不同产地佛手挥发油中化学成分；通过NIST 14.L数据库进行化合物检索并鉴别；采用峰面积归一化法测定各化
学成分的相对质量分数；采用SPSS 20.0软件对样品进行聚类分析。结果：10个不同产地佛手挥发油得率为0.10％～1.75％，其中
以四川乐山市犍为县样品最高（1.75％）。不同产地佛手挥发油共鉴别出66个成分，相对分子量为126.20～392.66，以C10和C15化
合物居多；相对分子量为136、154的同分异构体所占比例较大，主要为环烃基单萜烯类成分。不同产地佛手挥发油有12个共有成
分，按相对质量分数由大到小依次为柠檬烯（24.90％）、萜品烯（14.71％）、（－）-4 萜品醇（2.88％）、柠檬醛（2.33％）和α-没药烯
（2.33％）、香叶醇（1.52％）、α-蒎烯（1.37％）、反式佛手柑油烯（1.16％）、异松油烯（1.13％）、棕榈酸甲酯（1.12％）、芳樟醇（1.09％）、
乙酸香叶酯（1.04％）；其中8个为单萜烯类成分，2个为倍半萜烯类成分，2个为酯类成分。不同产地佛手可聚为4类，即S2、S4、S6
聚为一类，S1、S3、S7、S8聚为一类，S5、S10聚为一类，S9为一类。结论：不同产地佛手的挥发油成分存在一定的差异，同一成分的
含量在不同产地佛手挥发油中也存有差异。
关键词 佛手；挥发油；产地；气质联用法；成分

Comparative Analysis of the Components of Volatile Oil in Citrus medica from Different Producing Areas
2
1
1
ZHAO Yongyan ，ZHANG Junyin ，PENG Teng ，HE Peiyu ，KUANG Yu（1. College of Pharmacy，Chengdu
1
1
University of TCM & State Key Laboratory of TCM Resources with Southwest Characteristics & Key
Laboratory of Standardization of Chinese Medicines of Ministry of Education & State Key Laboratory
Cultivation Base of TCM Resource System Research and Utilization，Chengdu 611137，China；2. Dept. of
Pharmacy，Suining Hospital of TCM，Sichuan Suining 629000，China）
ABSTRACT OBJECTIVE：To compare the difference of the components of volatile oil in Citrus medica from different producing
areas. METHODS：The volatile oil of C. medica from 10 different producing areas was extracted with steam distillation，and the
yield was calculated. The components of the volatile oil of C. medica from different producing areas were analyzed by GC-MS. The
compounds were retrieved from NIST 14.L mass spectrum database and identified. Relative mass fraction of chemical component
was determined by peak area normalization method. Cluster analysis of samples were performed by using SPSS 20.0 software.
RESULTS：The yields of volatile oil of C. medica from 10 different producing areas were 0.10％-1.75％，among which sample
from Qianwei county in Leshan city of Sichuan province was the highest（1.75％）. A total of 66 components were identified in the

volatile oil of C. medica from different producing areas，with a relative molecular weight of 126.20-392.66. The majority was C10
and C15 compounds；isomers with relative molecular weight of 136，154 took up the great proportion，which were mainly
cycloalkane monoterpenes. There were 12 common components in the volatile oil of C. medica from different areas，which were
limonene（24.90％），terpinene（14.71％），（－）-4 terpineol（2.88％），citral（2.33％），α-myrrhene（2.33％），geraniol（1.52％），
α-pinene（1.37％），trans bergamot olene（1.16％），isoterpinene（1.13％），methyl palmitate（1.12％），linalool（1.09％）and geranyl
acetate（1.04％）according to relative mass fraction；8 of them were monoterpenes，2 were sesquiterpenes and 2 were esters. There
were 4 categories of C. medica from different producing areas，i.e. S2，S4，S6 clustered into one；S1，S3，S7，S8 clustered into one；
S5 and S10 clustered into one；S9 as one. CONCLUSIONS：
Δ 基金项目：国家自然科学基金资助项目（No.81673878）；四川省
There are some difference of the components in volatile oil of
科技计划项目（No.18ZDYF3402，No.2019YFS0024）；四川省青年科技
C. medica from different producing areas，and the content of
创新研究团队专项计划（No.2016TD0006）
the same component also has great difference in the volatile oil
＊硕士研究生。研究方向：中药有效成分分析及物质基础。电
of C. medica from different producing areas.
话：028-61800231。E-mail：1152743782@qq.com
# 通信作者：教授，硕士生导师，博士。研究方向：中药有效成分 KEYWORDS Citrus medica；Volatile oil；Producing area；
分析及物质基础。电话：028-61800231。E-mail：56649923@qq.com GC-MS；Component

中国药房 2020年第31卷第4期 China Pharmacy 2020 Vol. 31 No. 4 ·423 ·