Page 62 - 2019年2月第30卷第4期

知母皂苷B对缺氧/复氧损伤星形胶质细胞的保护作用及其机制

研究 Δ

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丁 玲 ，胡 毅 ，刘 洁 ，安瑞娣 ，张作文 （1.重庆市江津区中心医院药学部，重庆 402260；2.重庆市江津
区中心医院消化科，重庆 402260；3.甘肃省人民医院医保处，兰州 730000；4.重庆市江津区中心医院神经内
科，重庆 402260）

中图分类号 R285.5 文献标志码 A 文章编号 1001-0408（2019）04-0488-05
DOI 10.6039/j.issn.1001-0408.2019.04.12

摘 要 目的：探讨知母皂苷B对缺氧/复氧损伤星形胶质细胞的保护作用及其可能机制。方法：原代新生SD大鼠星形胶质细胞
经培养、鉴定后，随机分为正常组、模型组、阳性对照组（尼莫地平，10 μmol/L）以及知母皂苷B低、中、高剂量组（1、10、100 μmol/L）。正
常组和模型组均给予完全培养基1 000 μL，给药组给予含相应药物的完全培养基1 000 μL。除正常组外，其余各组细胞均采用氧糖
剥夺/再灌注法复制缺氧/复氧损伤模型。复氧后，采用比色法检测细胞中乳酸脱氢酶（LDH）的相对释放率；采用四甲基偶氮唑盐
法检测细胞相对活力；采用酶联免疫吸附测定法检测细胞中水通道蛋白 4（AQP-4）、白细胞介素 6（IL-6）、IL-1β、肿瘤坏死因子α
（TNF-α）的含量。结果：与正常组比较，模型组细胞中LDH相对释放率以及AQP-4、IL-6、IL-1β、TNF-α的含量均显著升高，细胞相
对活力显著降低（P＜0.01）。与模型组比较，各给药组细胞中LDH相对释放率以及AQP-4、IL-6、IL-1β、TNF-α的含量均显著下降，
细胞相对活力均显著升高（P＜0.05或P＜0.01）。结论：知母皂苷B可明显降低细胞损伤程度、增强细胞活力，对缺氧/复氧损伤星
形胶质细胞具有一定的保护作用，且这种作用可能与其下调AQP-4和IL-6、IL-1β、TNF-α的分泌有关。
关键词 知母皂苷B；星形胶质细胞；缺氧/复氧损伤；乳酸脱氢酶；水通道蛋白4；白细胞介素6；白细胞介素1β；肿瘤坏死因子α

Protective Effects and the Mechanism of Anemarsaponin B on Hypoxia/reoxygenation Injury Astrocytes
DING Ling ，HU Yi ，LIU Jie ，AN Ruidi ，ZHANG Zuowen（1. Dept. of Pharmacy，the Central Hospital of
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Jiangjin， Chongqing 402260， China； 2. Dept. of Gastroenterology， Chongqing Jiangjin District Central
Hospital，Chongqing 402260，China；3. Medical Insurance Office，Gansu Provincial People’s Hospital，
Lanzhou 730000，China；4. Dept. of Neurology，Chongqing Jiangjin District Central Hospital，Chongqing
402260，China）
ABSTRACT OBJECTIVE：To investigate the protective effects of anemarsaponin B on hypoxia/reoxygenation injury astrocytes
and its possible mechanism. METHODS：The primary astrocytes of neonatal SD rats were cultured and identified，and then
randomly divided into normal group，model group，positive control group（nimodipine，10 μmol/L），anemarsaponin B low-dose，
medium-dose and high-dose groups （1，10，100 μ mol/L），respectively. Normal group and model group were given complete
medium 1 000 μL. Administration group was given complete medium with relevant medicine 1 000 μL. Except for normal group，
hypoxia/reoxygenation injury model was established by oxygen-glucose deprivation/reperfusion in other groups. After
reoxygenation，relative release rate of lactate dehydrogenase（LDH）in cell was detected by colorimetry. MTT assay was used to
detect the relative viability of the cells. The contents of aquaporin 4（AQP-4），IL-6，IL-1β and TNF-α in cell were measured by
ELISA. RESULTS：Compared with normal group，relative release rate of LDH，the contents of AQP-4，IL-6，IL-1β and TNF-α in
cell were increased significantly in model group，while relative viability of the cells were decreased significantly （P＜0.01）.
Compared with model group，relative release rate of LDH，the contents of AQP-4，IL-6，IL-1β and TNF-α in cell were decreased
significantly in administration groups，while relative viability of the cells were increased significantly （P＜0.05 or P＜0.01）.
CONCLUSIONS：Anemarsaponin B can significantly decrease cell injury degree，strengthen cell viability and protect hypoxia/
reoxygenation injury astrocytes to certain extent. The effect
Δ 基金项目：重庆市教育委员会科学技术研究项目（No.KJ1600235）
may be related to the down-regulation of the secretion of
＊副主任药师，硕士。研究方向：药事管理、神经药理学。电话：
023-47225178。E-mail：626654306@qq.com AQP-4，IL-6，IL-1β and TNF-α.
# 通信作者：副主任医师，硕士。研究方向：脑血管疾病、多发性 KEYWORDS Anemarsaponin B； Astrocytes； Hypoxia/
硬化、癫痫等疾病诊疗。电话：023-47217495。E-mail：396096156@ reoxygenation injury；Lactate dehydrogenase；Aquaporin 4；
qq.com IL-6；IL-1β；TNF-α

·488 · China Pharmacy 2019 Vol. 30 No. 4 中国药房 2019年第30卷第4期