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基于非靶向代谢组学的兴安白芷不同部位差异代谢物分析
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王 星 ，周紫珊，田恩伟（南方医科大学中医药学院，广州 510515）
中图分类号 R931.6 文献标志码 A 文章编号 1001-0408（2026）09-1161-06
DOI 10.6039/j.issn.1001-0408.2026.09.09
摘 要 目的 探讨兴安白芷根、茎、叶的代谢物差异，为白芷药用资源的开发和利用提供参考。方法 采用超高效液相色谱-串联
质谱非靶向代谢组学技术对兴安白芷根、茎、叶进行代谢物检测。采用多元统计分析方法筛选差异代谢物并进行聚类分析，进一
步对差异代谢物进行京都基因与基因组百科全书（KEGG）通路富集分析。结果 在兴安白芷根、茎、叶中共检测出769种代谢物，
其中脂质类（14.31%）、生物碱类（13.91%）、氨基酸及其衍生物（13.65%）化合物占比较高，香豆素类化合物占比为3.25%。主成分
分析、正交偏最小二乘判别分析及聚类分析结果一致表明，根、茎、叶的代谢物存在一定差异。茎与根的差异代谢物共205种（82
种上调，123种下调），叶与根的差异代谢物共326种（247种上调，79种下调），叶与茎的差异代谢物共293种（263种上调，30种下
调）。进一步对不同部位中香豆素类差异代谢物进行分析发现，（R）-氧化前胡素、4-羟基香豆素、4-甲基伞形酮乙酸酯等在叶中的
相对含量较高。KEGG通路富集分析显示，差异代谢物主要富集于苯丙烷类物质生物合成、ABC转运蛋白等通路。结论 兴安白
芷根、茎、叶的代谢物存在较大差异。叶中香豆素类成分高度富集，其合成与转运可能与苯丙烷类生物合成、ABC转运蛋白通路
的协同作用相关。
关键词 兴安白芷；非药用部位；非靶向代谢组学；差异代谢物；资源开发；UPLC-MS/MS
Analysis of differential metabolites in different parts of Angelica dahurica （（Fisch. ex Hoffm.）） Benth. et
Hook. f. ex Franch. et Savat. based on untargeted metabolomics
WANG Xing，ZHOU Zishan，TIAN Enwei（School of Traditional Chinese Medicine， Southern Medical
University， Guangzhou 510515， China）
ABSTRACT OBJECTIVE To investigate the metabolic differences in roots， stems and leaves of Angelica dahurica （Fisch.ex
Hoffm.） Benth. et Hook. f. ex Franch. et Savat. （shortened for A. dahurica）， and to provide a basis for the development and
utilization of its medicinal resources. METHODS UPLC-MS/MS untargeted metabolomics technology was employed to detect
metabolites in roots， stems and leaves of A. dahurica. Multivariate statistical analysis was used to screen differential metabolites and
perform cluster analysis. Furthermore， Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis was
conducted on these differential metabolites. RESULTS A total of 769 metabolites were identified in roots， stems and leaves of A.
dahurica. Among them， lipids， alkaloids， amino acids and their derivatives accounted for the highest proportion， which accounted
for 14.31%， 13.91% and 13.65%， respectively， and the coumarin compounds accounted for 3.25%. Results of principal component
analysis， orthogonal partial least squares-discriminant analysis and cluster analysis indicated that there were certain differences in
metabolites among the three tissues. A total of 205 differential metabolites were identified between stems and roots （82 up-regulated
and 123 down-regulated）， 326 differential metabolites between leaves and roots （247 up-regulated and 79 down-regulated）， and
293 differential metabolites between the leaves and stems （263 up-regulated and 30 down-regulated）. Further analysis of the
differential coumarin metabolites in different plant parts revealed that several compounds， including （R）-oxypeucedanin， 4-
hydroxycoumarin， 4-methylumbelliferyl acetate， exhibited relatively high contents in the leaves. KEGG pathway enrichment
analysis indicated that differential metabolites predominantly accumulated in the phenylpropanoid biosynthesis and ABC transporter
pathways. CONCLUSIONS There are significant differences in metabolites among roots， stems and leaves of A. dahurica.
Coumarin compounds are highly enriched in the leaves， and their synthesis and transport may be related to the synergistic effect of
the phenylpropanoid biosynthesis pathway and the ABC transporter pathway.
KEYWORDS Angelica dahurica （Fisch. ex Hoffm.） Benth. et Hook. f. ex Franch. et Savat.； non-medicinal parts； untargeted
metabolomics； differential metabolites； resource development； UPLC-MS/MS
Δ 基金项目 广东省基础与应用基础研究基金项目（No.2024A-
白芷为伞形科植物白芷 Angelica dahurica（Fisch.
1515010875） ex Hoffm.）Benth. et Hook. f. 或杭白芷 A.dahurica（Fisch.
＊第一作者 硕士研究生。研究方向：中药质量评价。E-mail：
ex Hoffm.）Benth. et Hook. f. var. formosana（Boiss.）Shan
wangxing1022@126.com
# 通信作者 副教授，硕士生导师，博士。研究方向：中药质量评 et Yuan的干燥根，为我国常用中药材，具有解表散寒、祛
价。E-mail：tianenwei@126.com 风止痛、宣通鼻窍、燥湿止带、消肿排脓的功效，临床上
中国药房 2026年第37卷第9期 China Pharmacy 2026 Vol. 37 No. 9 · 1161 ·
