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裸花紫珠醇提物 UPLC 指纹图谱建立及抗呼吸道合胞病毒谱效

          关系研究
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          邵冰梅 ,李小锋 ,闫蓓蓓 ,刘启峰 ,秦焕云 ,赵祥君 ,侯 林 (1.山东中医药大学青岛中医药科学院,山东
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          青岛 266000;2.江西普正制药股份有限公司国家企业技术中心,江西 吉安 343100;3.山东中医药大学药学
          院,济南 250300;4.泰安市立医院神经外科,山东 泰安 271000)
          中图分类号  R917;R978.7      文献标志码  A      文章编号  1001-0408(2023)20-2465-06
          DOI  10.6039/j.issn.1001-0408.2023.20.04
          摘  要  目的  建立裸花紫珠醇提物的指纹图谱,分析其体外抗呼吸道合胞病毒(RSV)活性并进行谱效关系研究。方法  以10%、
          30%、50%、70%、90%乙醇为溶剂,制备4批裸花紫珠药材的20批醇提物。采用超高效液相色谱法,利用《中药色谱指纹图谱相似
          度评价系统(2012版)》绘制20批裸花紫珠醇提物的指纹图谱并进行相似度评价;采用致细胞病变效应法和MTT法考察裸花紫珠
          醇提物对 RSV 的体外抑制活性;运用 Pearson 相关性分析、灰色关联度分析、正交偏最小二乘(OPLS)分析进行谱效关系研究。
          结果  20批裸花紫珠醇提物有25个共有峰,相似度为0.912~0.998,共有峰峰面积的RSD为33.54%~162.28%。20批裸花紫珠醇
          提物对 RSV 的平均半数抑制浓度为 9.55~272.23 μg/mL;Pearson 相关性分析、灰色关联度分析、OPLS 分析结果表明,共有峰 8、
          10、12、16、18~19、22~24与药效指标的Pearson相关系数(P<0.05)和回归系数均为负值,关联度均大于0.6,变量重要性投影值
          均大于1。结论  20批裸花紫珠醇提物成分相似但含量差异明显,且具有不同程度的体外抗RSV活性,其中共有峰8、10、12、16、
          18~19、22~24对应成分可能是裸花紫珠抗RSV的特征成分。
          关键词  裸花紫珠;醇提物;呼吸道合胞病毒;指纹图谱;抗病毒;谱效关系


          Establishment  of  UPLC  fingerprint  and  study  on  anti-respiratory  syncytial  virus  spectrum-effective
          relationship of ethanol extract from Callicarpa nudiflora
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          SHAO Bingmei ,LI Xiaofeng ,YAN Beibei ,LIU Qifeng ,QIN Huanyun ,ZHAO Xiangjun ,HOU Lin
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         (1.  Qingdao  Academy  of  Chinese  Medicinal  Sciences,  Shandong  University  of  Traditional  Chinese  Medicine,
          Shandong  Qingdao  266000,  China;2.  National  Enterprise  Technology  Center,  Jiangxi  Puzheng  Pharmaceutical
          Co.,  Ltd.,  Jiangxi  Jiʼan  343100,  China;3.  School  of  Pharmacy,  Shandong  University  of  Traditional  Chinese
          Medicine,  Jinan  250300,  China;4.  Dept.  of  Neurosurgery,  Tai’an  Municipal  Hospital,  Shandong  Tai’an
          271000, China)
          ABSTRACT   OBJECTIVE  To  establish  the  fingerprint  of  the  ethanol  extract  from  Callicarpa  nudiflora,  analyze  its  anti-
          respiratory  syncytial  virus (RSV)  activity  in  vitro,  and  study  the  relationship  between  spectrum  and  effect.  METHODS  Using
          10%, 30%, 50%, 70% and 90% ethanol as solvent, 20 batches of ethanol extracts from 4 batches of C. nudiflora were prepared.
          The fingerprints for 20 batches of ethanol extracts from C. nudiflora were mapped by ultra-high-performance liquid chromatography
         (UPLC),  and  the  similarity  evaluation  was  conducted  by  using  the  Similarity  Evaluation  System  for  Traditional  Chinese  Medicine
          Chromatographic Fingerprints (2012 edition). The cytopathic effect method and MTT method were used to investigate the in vitro
          inhibitory  activity  of  the  ethanol  extracts  from  C.  nudiflora  on  RSV.  Pearson  correlation  analysis,  grey  correlation  degree  and
          orthogonal  partial  least  squares (OPLS)  analysis  were  used  to  study  the  spectrum-effect  relationship.  RESULTS  There  were  25
          common peaks in 20 batches of ethanol extracts from C. nudiflora, and the similarities ranged from 0.912 to 0.998, and the RSDs
          of  common  peak  areas  were  33.54%-162.28%.  The  average  values  of  IC50  for  RSV  of  20  batches  of  ethanol  extracts  from  C.
          nudiflora were 9.55-272.23 μg/mL. The results of Pearson correlation analysis, grey correlation analysis and OPLS analysis showed
          that  the  Pearson  correlation  coefficients (P<0.05)  of  the  common  peaks  8,  10,  12,  16,  18-19,  22-24  with  pharmacodynamic
                                                             indicators  and  regression  coefficients  were  all  negative,  the
             Δ 基金项目 山东省重点研发计划(重大科技创新工程)项目(No.
                                                             correlation  coefficients  were  all  greater  than  0.6,  and  the
          2021CXGC010511,No.2020CXGC010505)
                                                             values  of  variable  importance  in  projection  were  all  greater
             *第一作者 硕士研究生。研究方向:中药新药研发、抗感染中药
          药效及物质基础。E-mail:shaobingmei325@126.com              than  1.  CONCLUSIONS  Twenty  batches  of  ethanol  extracts
             # 通信作者 副教授,硕士生导师,博士。研究方向:中药新药研发                 from  C.  nudiflora  have  similar  components  but  significant
          及中成药二次开发、中医药大健康产品研发、抗感染中药药效及物质                     differences  in  content,  and  exhibit  different  degrees  of  anti-
          基础。E-mail:13789801721@163.com                      RSV  activity  in  vitro.  The  corresponding  components  of


          中国药房  2023年第34卷第20期                                              China Pharmacy  2023 Vol. 34  No. 20    · 2465 ·
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