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裸花紫珠醇提物 UPLC 指纹图谱建立及抗呼吸道合胞病毒谱效
关系研究
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邵冰梅 ,李小锋 ,闫蓓蓓 ,刘启峰 ,秦焕云 ,赵祥君 ,侯 林 (1.山东中医药大学青岛中医药科学院,山东
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青岛 266000;2.江西普正制药股份有限公司国家企业技术中心,江西 吉安 343100;3.山东中医药大学药学
院,济南 250300;4.泰安市立医院神经外科,山东 泰安 271000)
中图分类号 R917;R978.7 文献标志码 A 文章编号 1001-0408(2023)20-2465-06
DOI 10.6039/j.issn.1001-0408.2023.20.04
摘 要 目的 建立裸花紫珠醇提物的指纹图谱,分析其体外抗呼吸道合胞病毒(RSV)活性并进行谱效关系研究。方法 以10%、
30%、50%、70%、90%乙醇为溶剂,制备4批裸花紫珠药材的20批醇提物。采用超高效液相色谱法,利用《中药色谱指纹图谱相似
度评价系统(2012版)》绘制20批裸花紫珠醇提物的指纹图谱并进行相似度评价;采用致细胞病变效应法和MTT法考察裸花紫珠
醇提物对 RSV 的体外抑制活性;运用 Pearson 相关性分析、灰色关联度分析、正交偏最小二乘(OPLS)分析进行谱效关系研究。
结果 20批裸花紫珠醇提物有25个共有峰,相似度为0.912~0.998,共有峰峰面积的RSD为33.54%~162.28%。20批裸花紫珠醇
提物对 RSV 的平均半数抑制浓度为 9.55~272.23 μg/mL;Pearson 相关性分析、灰色关联度分析、OPLS 分析结果表明,共有峰 8、
10、12、16、18~19、22~24与药效指标的Pearson相关系数(P<0.05)和回归系数均为负值,关联度均大于0.6,变量重要性投影值
均大于1。结论 20批裸花紫珠醇提物成分相似但含量差异明显,且具有不同程度的体外抗RSV活性,其中共有峰8、10、12、16、
18~19、22~24对应成分可能是裸花紫珠抗RSV的特征成分。
关键词 裸花紫珠;醇提物;呼吸道合胞病毒;指纹图谱;抗病毒;谱效关系
Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective
relationship of ethanol extract from Callicarpa nudiflora
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SHAO Bingmei ,LI Xiaofeng ,YAN Beibei ,LIU Qifeng ,QIN Huanyun ,ZHAO Xiangjun ,HOU Lin
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(1. Qingdao Academy of Chinese Medicinal Sciences, Shandong University of Traditional Chinese Medicine,
Shandong Qingdao 266000, China;2. National Enterprise Technology Center, Jiangxi Puzheng Pharmaceutical
Co., Ltd., Jiangxi Jiʼan 343100, China;3. School of Pharmacy, Shandong University of Traditional Chinese
Medicine, Jinan 250300, China;4. Dept. of Neurosurgery, Tai’an Municipal Hospital, Shandong Tai’an
271000, China)
ABSTRACT OBJECTIVE To establish the fingerprint of the ethanol extract from Callicarpa nudiflora, analyze its anti-
respiratory syncytial virus (RSV) activity in vitro, and study the relationship between spectrum and effect. METHODS Using
10%, 30%, 50%, 70% and 90% ethanol as solvent, 20 batches of ethanol extracts from 4 batches of C. nudiflora were prepared.
The fingerprints for 20 batches of ethanol extracts from C. nudiflora were mapped by ultra-high-performance liquid chromatography
(UPLC), and the similarity evaluation was conducted by using the Similarity Evaluation System for Traditional Chinese Medicine
Chromatographic Fingerprints (2012 edition). The cytopathic effect method and MTT method were used to investigate the in vitro
inhibitory activity of the ethanol extracts from C. nudiflora on RSV. Pearson correlation analysis, grey correlation degree and
orthogonal partial least squares (OPLS) analysis were used to study the spectrum-effect relationship. RESULTS There were 25
common peaks in 20 batches of ethanol extracts from C. nudiflora, and the similarities ranged from 0.912 to 0.998, and the RSDs
of common peak areas were 33.54%-162.28%. The average values of IC50 for RSV of 20 batches of ethanol extracts from C.
nudiflora were 9.55-272.23 μg/mL. The results of Pearson correlation analysis, grey correlation analysis and OPLS analysis showed
that the Pearson correlation coefficients (P<0.05) of the common peaks 8, 10, 12, 16, 18-19, 22-24 with pharmacodynamic
indicators and regression coefficients were all negative, the
Δ 基金项目 山东省重点研发计划(重大科技创新工程)项目(No.
correlation coefficients were all greater than 0.6, and the
2021CXGC010511,No.2020CXGC010505)
values of variable importance in projection were all greater
*第一作者 硕士研究生。研究方向:中药新药研发、抗感染中药
药效及物质基础。E-mail:shaobingmei325@126.com than 1. CONCLUSIONS Twenty batches of ethanol extracts
# 通信作者 副教授,硕士生导师,博士。研究方向:中药新药研发 from C. nudiflora have similar components but significant
及中成药二次开发、中医药大健康产品研发、抗感染中药药效及物质 differences in content, and exhibit different degrees of anti-
基础。E-mail:13789801721@163.com RSV activity in vitro. The corresponding components of
中国药房 2023年第34卷第20期 China Pharmacy 2023 Vol. 34 No. 20 · 2465 ·