Page 59 - 《中国药房》2023年6期

火麻仁油改善UC模型大鼠的症状及对肠道菌群的影响
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龚梦鹃 ，高心悦，简晓敏，邹忠杰（广东药科大学中药学院，广州 510006）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2023）06-0693-06
DOI 10.6039/j.issn.1001-0408.2023.06.10

摘 要 目的 研究火麻仁油对右旋葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）模型大鼠症状的改善作用，并探讨其对大鼠肠
道菌群的影响。方法 将40只SD大鼠随机分为对照组、模型组、火麻仁油组（1 g/kg）和柳氮磺胺吡啶组（阳性对照，300 mg/kg），每组10
只。对照组和模型组大鼠灌胃0.5%聚山梨酯80，火麻仁油组和柳氮磺胺吡啶组大鼠灌胃相应药液，每天1次，连续10 d。从第4
天开始，模型组、火麻仁油组和柳氮磺胺吡啶组大鼠连续7 d饮用4%DSS溶液以建立UC模型。观察大鼠体质量、疾病活动指数
（DAI）评分、结肠长度、结肠质量、结肠单位长度质量、结肠组织病理学变化，检测其血清肿瘤坏死因子α（TNF-α）、白细胞介素6
（IL-6）、IL-10含量，通过高通量测序法检测各组大鼠肠道菌群的变化。结果 与对照组比较，模型组大鼠的体质量显著降低，结肠
长度显著缩短，DAI评分、结肠质量、结肠单位长度质量和血清TNF-α、IL-6含量均显著增加，IL-10含量显著降低（P＜0.05）；结肠
组织上皮层发生脱落，炎症细胞浸润且入侵黏膜下层，肠腺紊乱。与模型组比较，火麻仁油组和柳氮磺胺吡啶组大鼠上述指标均
显著逆转（P＜0.05），且相关症状均有明显改善。菌群测序结果显示，模型组大鼠的ACE指数和Chao1指数均较对照组显著降低
（P＜0.05），火麻仁油组大鼠的Chao1指数较模型组显著升高（P＜0.05）。与对照组比较，模型组有41个菌属发生改变；与模型组
比较，火麻仁油能使上述41个菌属中的3个菌属回调至正常状态，包括杜波西菌属、紫杆菌属和别样棒菌属。结论 火麻仁油能通
过调节肠道菌群多样性，增加有益菌和减少有害菌来改善UC模型大鼠症状。
关键词 火麻仁油；溃疡性结肠炎；肠道菌群；高通量测序；大鼠

Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats
GONG Mengjuan，GAO Xinyue，JIAN Xiaomin，ZOU Zhongjie（School of Traditional Chinese Medicine，
Guangdong Pharmaceutical University， Guangzhou 510006， China）

ABSTRACT OBJECTIVE To study the improvement effects of Cannabis sativa oil on the symptoms in dextran sodium sulfate
（DSS）-induced ulcerative colitis （UC） model rats， and to investigate its effects on intestinal flora of rats. METHODS Forty SD
rats were randomly divided into control group， model group， C. sativa oil group （1 g/kg） and sulfasalazine group （positive
control， 300 mg/kg）， with 10 rats in each group. The rats in control group and model group were given 0.5% polysorbate 80 by
gavage， and the rats in C. sativa oil group and sulfasalazine group were given corresponding drug solution by gavage once a day
for 10 days. From the 4th day， rats in model group， C. sativa oil group and sulfasalazine group were given 4% DSS solution for 7
consecutive days to establish UC model. The body weight， disease activity index （DAI） score， colon length， colon weight， weight
per unit length of colon， the pathological changes of colon tissue， and the contents of tumour necrosis factor- α （TNF- α），
interleukin-6 （IL-6） and IL-10 in serum of rats were determined. The changes of intestinal flora in rats were detected by high-
throughput sequencing. RESULTS Compared with control group， the body weight and the length of colon were decreased
significantly in model group， while DAI score， the weight of colon， weight per unit length of colon， serum contents of TNF-α and
IL-6 were increased significantly， and the content of IL-10 was decreased significantly （P＜0.05）； epithelial layer of colon tissue
fell off， inflammatory cells infiltrated and invaded the submucosa， and intestinal glands were disordered. Compared with model
group， above indexes of C. sativa oil group and sulfasalazine group were reversed significantly （P＜0.05）， and related symptoms
were improved significantly. The result of flora sequencing showed that ACE index and Chao1 index of model group were decreased
significantly， compared with control group （P＜0.05）； while Chao1 index of C. sativa oil group was increased significantly，
compared with model group （P＜0.05）. Compared with control group， 41 genera of bacteria in the model group changed；
compared with model group， C. sativa oil could return 3 of the 41 genera to normal state， including Dubosilla， Porphyrobacter and
Allobaculum. CONCLUSIONS C. sativa oil can improve the
Δ 基金项目 广 东 省 普 通 高 校 特 色 创 新 类 项 目（No. 2022-
symptoms of UC model rats by regulating the diversity of
KTSCX061）
intestinal flora， increasing beneficial bacteria and decreasing
＊第一作者 副教授，博士。研究方向：中药药理学。E-mail：
gongmengjuan@139.com pathogenic bacteria.
# 通信作者 教授，博士。研究方向：中药与肠道菌群。E-mail： KEYWORDS Cannabis sativa oil； ulcerative colitis；
zouzhongjie@139.com intestinal flora； high-throughput sequencing； rats

中国药房 2023年第34卷第6期 China Pharmacy 2023 Vol. 34 No. 6 · 693 ·