Page 55 - 《中国药房》2023年4期

栀子酒炙前后挥发油成分及保肝作用比较
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唐 怡 ，周立分 ，严志宏 ，邵 坚（1.江西省中西医结合医院药剂科，南昌 330003；2.江西中医药大学实验
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室服务中心，南昌 330004；3.江中药业股份有限公司，南昌 330096）
中图分类号 R917；R285 文献标志码 A 文章编号 1001-0408（2023）04-0433-05
DOI 10.6039/j.issn.1001-0408.2023.04.10

摘 要 目的 比较栀子酒炙前后挥发油成分及保肝作用。方法 采用水蒸气蒸馏法分别提取生栀子和酒栀子的挥发油，运用气
相色谱-质谱联用仪鉴定挥发油中的成分，用峰面积归一化法计算各成分的相对质量分数。将大鼠随机分为正常组，模型组，阳性
对照组（联苯双酯混悬液35 mg/kg），生栀子低、高剂量组[1、2 g/kg（以生药量计）]，酒栀子低、高剂量组[1、2 g/kg（以生药量计）]，每
组10只。各组大鼠连续灌胃相应药液7 d后腹腔注射40%四氯化碳建立肝损伤模型，比较各组大鼠状态、血清生化指标、肝组织
生化指标和肝组织病理切片。结果 鉴定出了生栀子挥发油成分23个，酒栀子挥发油成分25个，共有挥发油成分18个，其中17个
共有挥发油成分含量因酒炙而降低，1个共有挥发油成分含量因酒炙而升高。与模型组比较，各给药组大鼠萎靡症状和肝细胞损
伤均有不同程度改善；血清中腺苷脱氨酶、丙氨酸转氨酶、天冬氨酸转氨酶、直接胆红素、乳酸脱氢酶、前白蛋白、总胆汁酸、总胆红
素水平均显著降低，总蛋白水平均显著升高；肝组织中丙二醛水平均显著降低，差异均有统计学意义（P＜0.05或P＜0.01）。结论
生栀子炮制至酒栀子的过程中，有 17 个共有挥发油成分含量降低，有 1 个共有挥发油成分含量升高。酒栀子具有一定的保肝
作用。
关键词 栀子；挥发油；成分；含量；保肝作用

Comparison of volatile oil from Gardenia jasminoides and their liver protective effects before and after stir-
frying with wine
TANG Yi ，ZHOU Lifen ，YAN Zhihong ，SHAO Jian（1. Dept. of Pharmacy， Jiangxi Province Hospital of
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Integrated Chinese and Western Medicine， Nanchang 330003， China；2. Center for Laboratory Services， Jiangxi
University of Traditional Chinese Medicine， Nanchang 330004， China；3. Jiangzhong Pharmaceutical Co.， Ltd.，
Nanchang 330096， China）

ABSTRACT OBJECTIVE To compare the components of volatile oil from Gardenia jasminoides and their liver protective effect
before and after stir-frying with wine. METHODS Steam distillation was used to exact the volatile oil from G. jasminoides and
wine stir-fried G. jasminoides. The components of volatile oil were identified by GC-MS method， and the relative mass fraction of
each component was calculated by peak area normalization method. The rats were randomly divided into normal group， model
group， positive control group （bifendate suspension 35 mg/kg）， G. jasminoides low-dose and high-dose groups [1， 2 g/kg
（calculated by crude drug）] and wine stir-fried G. jasminoides low-dose and high-dose groups [1， 2 g/kg （calculated by crude
drug）] with 10 rats in each group. Liver injury model was established by intraperitoneal injection of 40% carbon tetrachloride in rats
of each group after continuous intragastric administration of corresponding drug solution for 7 days. The status， serum biochemical
indexes， liver biochemical indexes and liver pathological sections of rats in each group were compared. RESULTS Twenty-three
volatile oil components from G. jasminoides and 25 volatile oil components from wine stir-fried G. jasminoides were identified；
there were 18 common volatile oil components， of which the contents of 17 common components were decreased， while the
content of one common component was increased due to stir-frying with wine. Compared with model group， the symptoms of
depression and liver cell damage of rats in each administration group were improved to varying degrees； the serum levels of
adenosine deaminase， alanine transaminase， aspartate
Δ 基金项目 江西省中医药管理局科技计划（No.2020B0398） transaminase， direct bilirubin， lactate dehydrogenase，
＊第一作者 主管中药师，硕士。研究方向：中药分析、临床中药 prealbumin， total bile acid and total bilirubin were
学。E-mail：564694046@qq.com
significantly decreased， while the total protein level was
# 通信作者 讲师，博士研究生。研究方向：中药分析、中药质量控
制。E-mail：zhoulifen1103@163.com significantly increased； the level of malondialdehyde in liver

中国药房 2023年第34卷第4期 China Pharmacy 2023 Vol. 34 No. 4 · 433 ·