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不同煎煮时间下六味地黄方粉末饮片与传统饮片的指纹图谱和
多成分含量比较
Δ
1 #
吴 磊 ，刘玉平 ，陆 超 ，束雅春 （1.南京中医药大学附属医院药学部，南京 210029；2.中国药科大学中药
2
1＊
1
学院，南京 211198）
中图分类号 R917 文献标志码 A 文章编号 1001-0408（2023）01-0067-06
DOI 10.6039/j.issn.1001-0408.2023.01.13
摘 要 目的 比较六味地黄方粉末饮片与传统饮片（后文称为“粉末饮片”和“传统饮片”）提取过程中多成分的变化规律，为六味
地黄方的现代工艺研究提供科学依据。方法 以粉末饮片和传统饮片为样品，分别在浸泡60 min时，头煎0、5、10 、15 、20 、25、30、
40 、50、60 min时和二煎5、10、20、30、40 min时取样。采用高效液相色谱法建立不同煎煮时间下2种饮片的指纹图谱，进行相似度
评价和色谱峰的指认，并测定其中5-羟基糠醛、儿茶素、莫诺苷、马钱苷、獐芽菜苷、二氢槲皮素、丹皮酚和苯甲酰芍药苷8个指标
成分的含量。结果 不同煎煮时间下，2种饮片与各自对照指纹图谱R的相似度均大于0.98。在传统饮片指纹图谱中，共指认出5
个色谱峰，分别为5-羟基糠醛、莫诺苷、獐芽菜苷、二氢槲皮素、丹皮酚；在粉末饮片指纹图谱中，共指认出6个色谱峰，分别为5-羟
基糠醛、莫诺苷、獐芽菜苷、二氢槲皮素、丹皮酚、苯甲酰芍药苷。含量测定结果显示，在头煎过程中，前5 min内粉末饮片中几乎所
有成分的煎出速率均较传统饮片快； 40 min 后，除 5-羟基糠醛和丹皮酚外，其余活性成分的含量均低于传统饮片。在二煎过程
中，除丹皮酚和马钱苷外，其余成分的含量均是粉末饮片高于传统饮片；传统饮片中儿茶素在头煎中被完全煎出，而粉末饮片中儿
茶素在二煎中依然可被检测到。2种饮片中8种成分的总煎出量差异不大。结论 六味地黄方粉末饮片化学成分与传统饮片相比
无明显优势，无法节省煎煮时间和药材用量。
关键词 六味地黄方；粉末饮片；传统饮片；提取过程；指纹图谱；含量测定
Comparison of the fingerprint and multi-component contents between Liuwei dihuang powder decoction
pieces and traditional decoction pieces with different decocting time
WU Lei ，LIU Yuping ，LU Chao ，SHU Yachun（1. Dept. of Pharmacy， the Affiliated Hospital of Nanjing
1
1
1
2
University of Chinese Medicine， Nanjing 210029， China；2. School of TCM， China Pharmaceutical University，
Nanjing 211198， China）
ABSTRACT OBJECTIVE To compare the change law of multi-components in the extraction process between Liuwei dihuang
powder decoction pieces and traditional decoction pieces （hereinafter referred to as powder decoction pieces and traditional
decoction pieces）， and to provide scientific basis for the modern technology research of Liuwei dihuang formula. METHODS
Taking powder decoction pieces and traditional decoction pieces as samples， the samples were taken when soaking for 60 min， at
0， 5， 10， 15， 20， 25， 30， 40， 50， 60 min of the first decocting and at 5， 10， 20， 30， 40 min of the second decocting，
respectively. HPLC method was used to establish the fingerprints of 2 kinds of decoction pieces with different decocting time. The
similarity evaluation and peak identification were performed. The contents of 8 components including 5-hydroxyfurfural， catechin，
monoglycoside， loganin， swertin glycoside， dihydroquercetin， paeonol and benzoyl paeoniflorin were all determined. RESULTS
With different decocting time， the similarties between 2 kinds of decoction pieces and their respective control fingerprints R were
all greater than 0.98. In the fingerprints of traditional decoction pieces， five chromatographic peaks were identified， namely， 5-
hydroxyfurfural， monetin， swertiaoside， dihydroquercetin and paeonol； in the fingerprints of powder decoction pieces， six
chromatographic peaks were identified， namely， 5-hydroxyfurfural， monoglycoside， swertiamarin， dihydroquercetin， paeonol and
benzoyl paeoniflorin. The results of content determination showed that in the first 5 minutes of the first decocting， the decocting
rate of almost all the ingredients in the powder decoction pieces was faster than that of the traditional decoction pieces； after 40
min， the contents of other active ingredients were lower than those of traditional decoction pieces except for 5-hydroxyfurfural and
paeonol. In the process of second decocting， except for
Δ 基金项目 2020 年 度 院 级 创 新 发 展 基 金 专 项 课 题（No. paeonol and loganin， the contents of other ingredients in
Y2020CX27）；江苏省药学会-天晴医院药学基金项目（No.Q202009） powder decoction pieces were higher than that in traditional
＊第一作者 副主任中药师，博士。研究方向：中药制剂与分析。
E-mail：xiaoleiyaoshi@163.com decoction pieces； catechin was completely decocted from the
# 通信作者 主任中药师，博士。研究方向：中药制剂与分析。 traditional decoction pieces in the first decocting， while it
E-mail：yfy0093@njucm.edu.cn could still be detected in the powder decoction pieces in the
中国药房 2023年第34卷第1期 China Pharmacy 2023 Vol. 34 No. 1 · 67 ·
