Page 35 - 《中国药房》2023年1期
P. 35

知母活性成分对脓毒症性心肌损伤模型小鼠的改善作用
                                                                                                    Δ


                                                                                    1， 2 #
                                                       1， 2
                                     1， 2
                           1， 2
                                                                1， 2
          李 倩    1， 2＊ ，陈香茗 ，常鑫鑫 ，崔文华 ，李 阳 ，薛紫鲸 ，郑玉光                      1， 2， 3 ，宋军娜 （1.河北中医学院药学院/
                                              1
          河北省中药炮制技术创新中心，石家庄 050091；2.河北省中药资源利用与质量评价国际联合研究中心，石家
          庄  050091；3.河北化工医药职业技术学院，石家庄  050026）
          中图分类号  R965      文献标志码  A      文章编号  1001-0408（2023）01-0029-06
          DOI  10.6039/j.issn.1001-0408.2023.01.06

          摘  要  目的  为阐明知母改善脓毒症性心肌损伤的作用及可能的活性物质基础提供实验参考。方法 采用热回流法制备知母水
          提物，大孔吸附树脂法分离知母总双苯吡酮和总皂苷。采用腹腔注射脂多糖建立脓毒症性心肌损伤小鼠模型，考察知母3个提取
          部位及知母单体成分芒果苷、知母皂苷AⅢ、知母皂苷BⅡ对模型小鼠存活率的影响；HE染色法观察知母单体成分芒果苷、知母皂
          苷AⅢ、知母皂苷BⅡ对模型小鼠心肌组织形态的影响；检测芒果苷对模型小鼠心肌组织中炎症因子[白细胞介素6（IL-6）、IL-1β
          和肿瘤坏死因子α（TNF-α）] mRNA表达及活性氧（ROS）水平的影响。结果  知母总双苯吡酮、总皂苷、水提物干预组小鼠存活率
          较模型组均不同程度升高，其中以知母总双苯吡酮部位效果较佳；芒果苷、知母皂苷AⅢ和知母皂苷BⅡ均可改善模型小鼠心肌
          细胞肿胀、肌束排列紊乱程度，且以芒果苷的效果更为明显。芒果苷干预后小鼠心肌组织中IL-6、IL-1β、TNF-α mRNA表达水平
          及ROS水平较模型组不同程度降低。结论  知母不同提取部位均可提高脓毒症性心肌损伤小鼠的存活率，其中以知母总双苯吡
          酮部位效果较佳。3个知母单体成分中以芒果苷改善脓毒症性心肌损伤的效果最好，其可能是通过抗炎、抗氧化发挥的作用。
          关键词  知母；总双苯吡酮；总皂苷；活性成分；芒果苷；脓毒症性心肌损伤

          Improvement effect of active ingredients of Anemarrhena asphodeloides on sepsis-induced myocardial injury
          model mice
                                                                                                  1， 2
          LI Qian ，CHEN Xiangming ，CHANG Xinxin ，CUI Wenhua ，LI Yang ，XUE Zijing ，ZHENG
                                                                                   1， 2
                                                                        1
                                                         1， 2
                                      1， 2
                 1， 2
          Yuguang 1， 2， 3 ，SONG Junna （1. College of Pharmacy，Hebei University of Chinese Medicine/Traditional Chinese
                                 1， 2
          Medicine Processing Technology Innovation Center of Hebei Province，Shijiazhuang 050091， China； 2. International
          Joint  Research  Center  on  Resource  Utilization  and  Quality  Evaluation  of Traditional  Chinese  Medicine  of  Hebei
          Province，Shijiazhuang  050091，China；3.  Hebei  Chemical  and  Pharmaceutical  College，Shijiazhuang  050026，
          China）
          ABSTRACT   OBJECTIVE  To  provide  reference  for  clarifying  improvement  effects  of  Anemarrhena  asphodeloides  on  sepsis-
          induced  myocardial  injury  and  potential  material  basis.  METHODS  Water  extract  of  A.  asphodeloides  was  extracted  by  thermal
          reflux  method.  Total  xanthone  and  total  saponins  in  A.  asphodeloides  were  separated  by  macroporous  adsorption  resin.  The  mice
          model  of  sepsis-induced  myocardial  injury  was  established  by  intraperitoneal  injection  of  lipopolysaccharide.  The  effects  of  the
          location of  three extraction fractions and the monomers of A.  asphodeloides  as  mangiferin，  timosaponin AⅢ  and timosaponin BⅡ
          on the survival rate of the model mice were explored. HE staining was used to observe the effects of mangiferin， timosaponin AⅢ
          and  timosaponin  BⅡ  on  myocardial  morphology  in  model  mice. The  effects  of  mangiferin  on  mRNA  expressions  of  inflammatory
          cytokines  [interleukin-6 （IL-6），  IL-1β  and  tumor  necrosis  factor-α （TNF-α）]  and  the  level  of  reactive  oxygen  species （ROS）  in
          myocardial  tissue  of  model  mice  were  detected.  RESULTS  Compared  with  the  model  group，  the  survival  rate  of  mice  in  the
          intervention  group  of  total  xanthone，  total  saponins  and  water  extract  was  increased  to  different  extents，  especially  total  xanthone
          fraction.  Mangiferin，  timosaponin  A Ⅲ  and  timosaponin  B Ⅱ could  improve  the  degree  of  myocardial  cell  swelling  and  muscle
          bundle  arrangement  disorder  in  model  mice，  especially  mangiferin.  Compared  with  model  group，  mRNA  expressions  of  IL-6，  IL-
          1β  and  TNF- α，  ROS  level  in  myocardium  of  mice  after  mangiferin  intervention  were  decreased  to  different  extents.
          CONCLUSIONS  The  different  extraction  fractions  of  A.  asphodeloides  can  improve  survival  rate  of  mice  with  sepsis-induced
          myocardial  injury，  especially  total  xanthone  fraction.  Mangiferin  is  the  best  among  the  three  monomers  of  A.  asphodeloides  to
                                                             improve  sepsis-induced  myocardial  injury，  which  may  play  a
             Δ 基金项目 国家自然科学基金资助项目（No.81803762）；河北省
                                                             role  in  anti-sepsis  myocardial  injury  by  anti-inflammation  and
          自然科学基金资助项目（No.H2022423317）
                                                             antioxidantion.
             ＊第一作者 硕士研究生。研究方向：中药质量分析及活性成分药
          效。E-mail：qianli_1231@163.com                       KEYWORDS     Anemarrhenae  asphodeloides；  total  xanthone；
             # 通信作者 副教授，硕士生导师。研究方向：中药质量分析及活                  total  saponins；  active  ingredients；  mangiferin；  sepsis-induced
          性成分对心血管疾病的防治。E-mail：junnasong@163.com              myocardial injury


          中国药房  2023年第34卷第1期                                                  China Pharmacy  2023 Vol. 34  No. 1    · 29 ·
   30   31   32   33   34   35   36   37   38   39   40