Page 57 - 《中国药房》2022年5期

怀中 1 号地黄不同炮制品中脂溶性成分比较及体外抗氧化活性

研究 Δ

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李 孟 1，2＊ ，杨 颖 ，邓晓颜 ，张靖柯 ，王胜超 ，孙孝亚 ，郑晓珂 ，冯卫生 （1.河南中医药大学药学院，
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郑州 450046；2.河南省中药开发工程技术研究中心，郑州 450046）
中图分类号 R284；R285 文献标志码 A 文章编号 1001-0408（2022）05-0563-07
DOI 10.6039/j.issn.1001-0408.2022.05.09
摘 要 目的 比较怀中1号地黄不同炮制品（鲜地黄、生地黄和熟地黄）中脂溶性成分的差异，并初步评价其体外抗氧化活性。方
法 采用索氏提取法获得地黄3种炮制品的脂溶性提取物，采用气相色谱-串联质谱法对其进行成分分析。采用NIST98系统谱库
自动检索组分的质谱信息，结合有关文献和与八峰索引、EPA/NIH库对照对化合物进行结构鉴定，并使用Hewlett Packard软件按
峰面积归一化法计算各化合物的相对含量。采用1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除法考察3种地黄炮制品中脂溶性
成分的抗氧化活性。结果 从地黄不同炮制品中共鉴定出79个脂溶性成分，从鲜地黄、生地黄和熟地黄中分别鉴定出48、52和37
个脂溶性成分，其相对含量分别占总成分的92.69％、86.29％、92.89％；三者中共有脂溶性成分20个，其相对含量分别占各炮制品
中脂溶性成分含量的88.73％、80.89％、85.87％，且均以亚油酸甲酯、棕榈酸甲酯、油酸甲酯等脂肪酸类成分为主。另外，鲜地黄中
独有的脂溶性成分有18个，多为萜类化合物；生地黄和熟地黄中独有的脂溶性成分分别有17、6个，多为烷烃类化合物。抗氧化实
验结果显示，三者中脂溶性成分对DPPH自由基的半数清除浓度分别为0.756、0.660、0.758 mg/mL。结论 不同地黄炮制品中的共
有脂溶性成分多为脂肪酸类化合物，鲜地黄中独有的脂溶性成分多为萜类化合物，生地黄和熟地黄中独有的脂溶性成分多为烷烃
类化合物；其脂溶性成分均具有一定的体外抗氧化活性。
关键词 怀中1号地黄；鲜地黄；生地黄；熟地黄；脂溶性成分；气相色谱-串联质谱法；抗氧化
Comparison of liposoluble constituents and study on in vitro antioxidant activities of different processed
products of Huaizhong No.1 Rehmannia glutionsa
LI Meng ，YANG Ying ，DENG Xiaoyan ，ZHANG Jingke ，WANG Shengchao ，SUN Xiaoya ，ZHENG
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Xiaoke ，FENG Weisheng （1. School of Pharmacy，Henan University of Chinese Medicine，Zhengzhou
450046，China；2. Engineering and Technology Center for Chinese Medicine Development of Henan Province，
Zhengzhou 450046，China）
ABSTRACT OBJECTIVE To compare the difference of liposoluble constituents in different processed products of Huaizhong
No.1 Rehmannia glutionsa（fresh R. glutionsa，R. glutionsa and prepared R. glutionsa），and to evaluate its in vitro antioxidant
activity preliminarily. METHODS Liposoluble extracts were extracted from 3 processed products of R. glutionsa by Soxhlet
extraction. Their constituents were analyzed by gas chromatography-mass spectrometry. The spectral library of NIST98 system was
used to automatically retrieve the mass spectrum information of components，and the structures of compounds were identified in
combination with relevant literature and by comparing with eight peak index and EPA/NIH library. Relative contents of the
components were calculated by using peak area normalization method with Hewlett Packard software. The antioxidant activities of
liposoluble constituents in 3 processed products of R. glutionsa were investigated by 1，1-diphenyl-2-picrylhydrazyl（DPPH）free
radical scavenging. RESULTS A total of 79 liposoluble components were identified from different processed products of R.
glutionsa，and 48，52 and 37 liposoluble compounds were identified from fresh R. glutionsa，R. glutionsa and prepared R.
glutionsa，respectively；their relative contents accounted for 92.69％ ，86.29％ ，92.89％ of the total components respectively.
Among them，there were 20 liposoluble compounds totally，and their relative contents accounted for 88.73％，80.89％ and 85.87％
of liposoluble components in each processed product respectively；they were mainly composed of fatty acids such as methyl
linoleate，methyl palmitate and methyl oleate. In addition，there were 18 unique liposoluble components in fresh R. glutionsa，
mostly terpenoids；there were 17 and 6 unique liposoluble components in R. glutionsa and prepared R. glutionsa，mostly alkanes.
The results of antioxidant experiment showed that median
Δ 基金项目：国家重点研发计划中医药现代化研究重点专项
scavenging concentrations of liposoluble components to DPPH
（No.2017YFC1702800）
free radical were 0.756，0.660，0.758 mg/mL，respectively.
＊讲师，博士。研究方向：中药药效物质基础。E-mail：limeng
31716@163.com CONCLUSIONS The common liposoluble components in
# 通信作者：教授，博士。研究方向：中药药效物质基础。E-mail： different processed products of R. glutionsa are mostly fatty
fwsh@hactcm.edu.cn acids；the unique liposoluble components in fresh R. glutionsa

中国药房 2022年第33卷第5期 China Pharmacy 2022 Vol. 33 No. 5 ·563 ·