Page 33 - 2021年18期

基于气味信息的荆芥穗产地鉴别 Δ

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张礼欣 ，于小聪 ，王泽琨 ，毛春琴 ，束雅春 （1.南京中医药大学附属医院药学部，南京 210029；2.南京中
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医药大学药学院，南京 210023）
中图分类号 R282.5 文献标志码 A 文章编号 1001-0408（2021）18-2203-07
DOI 10.6039/j.issn.1001-0408.2021.18.05

摘 要 目的：建立基于气味信息的不同产地荆芥穗的鉴别方法。方法：采用Heracles Ⅱ型超快速气相电子鼻技术对不同产地荆
芥穗进行气味鉴别，根据获取的图谱信息结合 AroChemBase 数据库、Kovates 保留指数定性库进行定性分析；采用 Alpha Soft
V14.2软件进行主成分分析（PCA）、判别因子分析（DFA）；采用SPSS 22.2软件进行聚类分析（CA）。结果：15批不同产地荆芥穗中
共有16个共有峰，经与AroChemBase数据库、Kovates保留指数定性库对比，共得到13个可能存在的成分。不同产地荆芥穗可能
存在的成分及感官描述信息基本相同，仅含量存在差别。2号共有峰的色谱峰强度为安徽＞甘肃＞河南＞河北＞江苏；6号共有
峰的色谱峰强度为安徽＞河北＞甘肃≈河南＞江苏；9号共有峰的色谱峰强度为安徽＞甘肃＞河南＞江苏＞河北；13号共有峰的
色谱峰强度为安徽≈甘肃＞河北＞江苏＞河南，即代表甲酸甲酯（2号）、α-蒎烯（6号）、3-壬酮（9号）及α-松油醇（13号）这4种成分
的色谱峰强度可因产地变化而存在较为显著的差异。 PCA结果显示，前2个主成分的累计贡献率为96.807％。DFA结果显示，判
别因子1和判别因子2的贡献率分别为92.089％和3.982％。CA结果显示，当距离为10时，15批样品可聚为3类，B1～B5、J1～J3
聚为一类，A1～A3聚为一类，G1、G2、N1、N2聚为一类，与 PCA、DFA结果基本一致。结论：超快速气相电子鼻技术可用于鉴别不
同产地的荆芥穗；甲酸甲酯、α-蒎烯、3-壬酮及α-松油醇可能为区分不同产地荆芥穗的关键因素。
关键词 超快速气相电子鼻技术；荆芥穗；不同产地；化学计量学；气味鉴别

Identification of the Origin of Schizonepeta tenusfolia Based on“Odor”Information
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ZHANG Lixin ，YU Xiaocong ，WANG Zekun ，MAO Chunqin ，SHU Yachun （1. Dept. of Pharmacy，the
Affiliated Hospital of Nanjing University of TCM，Nanjing 210029，China；2. School of Pharmacy，Nanjing
University of TCM，Nanjing 210023，China）
ABSTRACT OBJECTIVE：To establish the method for identifying Schizonepeta tenusfolia from different habitats based on odor
information. METHODS：The odor of S. tenusfolia from different habitats were identified by Heracles Ⅱ ultra-fast gas phase
electronic nose. Qualitative analysis was conducted according to obtained chromatographic information combined with
AroChemBase database and Kovats retention index qualitative database. Principle component analysis （PCA） and discriminant
factor analysis（DFA）were conducted by using Alpha Soft V14.2 software，and cluster analysis（CA）was performed with SPSS
22.2 software. RESULTS：There were 16 common peaks in 15 batches of S. tenusfolia from different habitats. After comparison
with AroChemBase database and Kovates retention index qualitative database，a total of 13 possible components were obtained. The
possible components and sensory description information of S. tenusfolia from different habitats were basically the same，but only
the content was different. The chromatographic peak intensities of common peak No. 2 were in descending order as Anhui ＞
Gansu＞Henan＞Hebei＞Jiangsu，the chromatographic peak intensities of common peak No. 6 were in descending order as Anhui＞
Hebei＞Gansu≈Henan＞Jiangsu，the chromatographic peak intensities of common peak No. 9 were in descending order as Anhui＞
Gansu＞Henan＞Jiangsu＞Hebei，the chromatographic peak intensity of common peak No. 13 were in descending order as Anhui≈
Gansu＞Hebei＞Jiangsu＞Henan，which represented the chromatographic peak intensity of methyl formate（peak No. 2），α-pinene
（peak No. 6），3-nonone（peak No. 9）and α-terpineol（peak No. 13）were significantly different due to the change of habitats.
PCA results showed that the cumulative contribution rate of the first two principal components was 96.807％ . Results of DFA
showed that contribution rates of discriminant factor 1 and discriminant factor 2 were 92.089％ and 3.982％. CA results showed that
when the distance was 10，15 batches of samples could be clustered into 3 categories，B1-B5 and J1-J3 into one category，A1-A3
into one category，G1，G2，N1 and N2 into one category. The results were basically consistent with those of PCA and DFA.
CONCLUSIONS：Ultra-fast gas phase electronic nose technology can be used to identify S. tenusfolia from different habitats
rapidly. Methyl formate，α-pinene，3-nonone and α-terpineol
Δ 基金项目：国家重点研发计划项目（No.2018YFC1707000）；国
may be the key factors to distinguish S. tenusfolia from
家自然科学基金资助项目（No.81503216）
different habitats.
＊硕士研究生。研究方向：中药炮制及质量标准。电话：025-
86617141。E-mail：857545808@qq.com KEYWORDS Ultra-fast gas phase electronic nose；Schizone-
# 通信作者：副教授，硕士生导师，博士。研究方向：中药炮制及 petae tenusfolia； Different habitats； Chemometrics； Odor
质量标准。电话：025-86617141。E-mail：yfy0093@njucm.edu.cn identification

中国药房 2021年第32卷第18期 China Pharmacy 2021 Vol. 32 No. 18 ·2203 ·