Page 55 - 《中国药房》2021年16期

地稔醇提物抗氧化活性的谱效关系研究 Δ

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钱 松 ，刘 琴，麻秀萍 ，杨 菁，陈 滕，刘 静，刘林莉（贵州中医药大学药学院，贵阳 550025）
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中图分类号 R284.1；R285 文献标志码 A 文章编号 1001-0408（2021）16-1969-06
DOI 10.6039/j.issn.1001-0408.2021.16.09

摘 要 目的：建立地稔醇提物的指纹图谱，研究其抗氧化活性的谱效关系。方法：采用高效液相色谱法（HPLC）和《中药色谱指
纹图谱相似度评价系统（2012版）》建立15批地稔醇提物的指纹图谱并进行相似度评价；与对照品比对，指认共有峰。采用1，1-二
苯基-2-三硝基苯肼（DPPH）自由基清除法、2，2’-联氮-二（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基清除法和总抗氧化能
力测定法（FRAP）评价15批地稔醇提物的体外抗氧化活性。采用主成分分析、双变量相关性分析和偏最小二乘回归分析研究地
稔醇提物抗氧化活性的谱效关系。结果：从15批地稔醇提物HPLC指纹图谱中确定共有峰20个，与对照指纹图谱的相似度均不
低于0.831；指认出峰3为没食子酸、峰13为牡荆素、峰17为芦丁、峰19为鞣花酸。15批地稔醇提物的DPPH自由基清除法的半数
抑制浓度（IC50）为 21.98～57.87 μg/mL，ABTS 自由基清除法的 IC50 值为 40.94～101.88 μg/mL，FRAP 法所得结果为 0.19～0.48
mg/mL。主成分分析显示，DPPH法的IC50值的方差贡献率达80.77％；双变量相关性分析显示，峰2（呈正相关）和峰11（呈负相关）
的峰面积与抗氧化活性均有显著的相关性（P＜0.05）；偏最小二乘回归法分析显示，变量重要性投影（VIP）值排序为峰11＞峰2＞
峰16＞峰15＞峰12＞峰13＞峰18，均大于1；且峰2、13、15、16、18与抗氧化活性呈正相关，峰11、12与抗氧化活性呈负相关，其标
准化回归系数的绝对值均大于0.1。结论：15批地稔醇提物均有体外抗氧化活性，其共有峰2、11、12、13、15、16、18对应的化合物
可能是地稔醇提物抗氧化活性的物质基础。
关键词 地稔；醇提物；指纹图谱；抗氧化活性；谱效关系

Study on the Spectrum-effect Relationship of Antioxidant Activity of Ethanol Extract from Melastoma
dodecandrum
QIAN Song，LIU Qin，MA Xiuping，YANG Jing，CHEN Teng，LIU Jing，LIU Linli（School of Pharmacy，
Guizhou University of TCM，Guiyang 550025，China）

ABSTRACT OBJECTIVE： To establish the fingerprint of the ethanol extract from Melastoma dodecandrum， to study
spectrum-effect relationship of its antioxidant activity. METHODS：HPLC method and Similarity Evaluation System of TCM
Chromatographic Fingerprint （2012 edition） were used to establish the fingerprints of 15 batches of ethanol extracts from M.
dodecandrum，and heir similarity was evaluated. The common peaks were identified by comparing with substance control. DPPH
free radical scavenging method，ABTS free radical scavenging method and total antioxidant capacity determination method（FRAP）
were used to determine antioxidant activity in vitro of 15 batches of ethanol extracts from M. dodecandrum. Principal component
analysis，bivariate correlation analysis and partial least squares regression analysis were used to study the spectrum-effect
relationship of the antioxidant activity of the ethanol extracts from M. dodecandrum. RESULTS：Totally 20 common peaks were
identified in HPLC fingerprints of 15 batches of ethanol extracts from M. dodecandrum；its similarity with the control fingerprint
was not less than 0.831；it was identified that peak 3 was gallic acid，peak 13 was vitexin，peak 17 was rutin and peak 19 was
ellagic acid. The IC50 values of DPPH radical scavenging method of 15 batches of ethanol extracts from M. dodecandrum were
21.98-57.87 μ g/mL，that of ABTS radical scavenging method were 40.94-101.88 μ g/mL，the results of FRAP method were
0.19-0.48 mg/mL. Principal component analysis showed that the contribution rate of IC50 variance of DPPH was 80.77％. Bivariate
correlation analysis showed that the peak areas of peak 2 （positive correlation） and peak 11 （negative correlation） were
significantly correlated with antioxidant activity （P＜0.05）；partial least squares regression analysis showed that，the variable
projection importance（VIP）in descending order was peak 11＞peak 2＞peak 16＞peak 15＞peak 12＞peak 13＞peak 18，and
their VIP values were greater than 1. Peaks 2，13，15，16 and 18 were positively correlated with antioxidant activity，and peaks 11
and 12 were negatively correlated with antioxidant activity，and the absolute value of standardized regression coefficient were
greater than 0.1. CONCLUSIONS：Fifteen batches of ethanol
Δ 基金项目：国家自然科学基金资助项目（No.81860695）；贵州省
extracts of M. dodecandrum have antioxidant activity in vitro.
药物新剂型新工艺科技创新人才团队项目（No.黔科合平台人才
The compounds corresponding to common peaks 2，11，12，
〔2017〕5655）
13，15，16 and 18 may be the material basis of antioxidant
＊硕士研究生。研究方向：中药及民族药质量控制及新药研发。
activity of M. dodecandrum.
E-mail：2371229201@qq.com
# 通信作者：教授，硕士生导师。研究方向：中药及民族药质量控 KEYWORDS Melastoma dodecandrum； Ethanol extract；
制及新药研发。E-mail：mxp001130@sina.com Fingerprint；Antioxidant activity； Spectrum-effect relationship

中国药房 2021年第32卷第16期 China Pharmacy 2021 Vol. 32 No. 16 ·1969 ·