Page 45 - 《中国药房》2021年07期

青稞多糖对糖尿病模型小鼠的降血糖作用及机制研究 Δ

王生亚 ，薛 洁 ，徐乃玉 ，张真庆 ，吕 栋 （1.南京医科大学附属无锡儿童医院药学部，江苏 无锡
3 #
1＊
2
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214023；2.苏州大学医学部药学院，江苏 苏州 215123；3.南京医科大学附属无锡人民医院药学部，江苏 无锡
214023）
中图分类号 R285.5 文献标志码 A 文章编号 1001-0408（2021）07-0807-05
DOI 10.6039/j.issn.1001-0408.2021.07.07

摘 要 目的：研究青稞多糖（HVP）对糖尿病模型小鼠的降血糖作用及机制。方法：对小鼠腹腔注射链脲佐菌素（120 mg/kg）以
复制糖尿病模型。将造模成功的小鼠随机分为模型组、二甲双胍组（阳性对照，200 mg/kg）和HVP高、中、低剂量组（300、150、75
mg/kg），另设空白对照组，每组10只。各给药组灌胃相应药物，空白对照组和模型组小鼠灌胃等量水，每天1次，连续30 天。给药
10、20、30 天时测定小鼠的空腹血糖（FBG）；末次FBG测定后，小鼠腹腔注射10％葡萄糖溶液（2 g/kg），于注射后30、120 min时测
定糖耐受量（GTT）曲线下面积；测定小鼠血清中胰岛素水平和肝组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、
丙二醛（MDA）的含量；采用苏木精-伊红染色法观察小鼠胰腺组织病理学变化。结果：与空白对照组比较，模型组小鼠FBG、GTT
曲线下面积和肝组织中 MDA 含量均显著升高或增加（P＜0.01）；血清中胰岛素水平和肝组织中 SOD、GSH-Px 含量均显著降低
（P＜0.01）；胰腺组织损伤严重，形态不完整，有明显的空泡。与模型组比较，HVP高剂量组小鼠FBG、GTT曲线下面积和肝组织
中MDA含量均显著降低或减少（P＜0.05或P＜0.01），血清中胰岛素含量和肝组织中SOD、GSH-Px含量均显著升高（P＜0.05或
P＜0.01），HVP低、中剂量组上述指标部分改善，差异具有统计学意义（P＜0.05或P＜0.01）；胰腺组织损伤有所减轻，形态较为完
整，胰岛细胞排列紧密，空泡减少。结论：HVP可降低糖尿病模型小鼠的血糖水平，升高胰岛素水平并减轻胰腺组织损伤；该作用
机制可能与其抗氧化作用有关。
关键词 青稞多糖；糖尿病；抗氧化；小鼠

Study on Hypoglycemic Effect of Hordeum vulgare Polysaccharide on Diabetes Mellitus Model Mice and
Its Mechanism
WANG Shengya ，XUE Jie ，XU Naiyu ，ZHANG Zhenqing ，LYU Dong（1. Dept. of Pharmacy，Wuxi Children’s
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Hospital Affiliated to Nanjing Medical University，Jiangsu Wuxi 214023，China；2. College of Pharmaceutical
Sciences， Health Science Center of Soochow University， Jiangsu Suzhou 215123， China； 3. Dept. of
Pharmacy，Wuxi People’s Hospital Affiliated to Nanjing Medical University，Jiangsu Wuxi 214023，China）
ABSTRACT OBJECTIVE：To study the hypoglycemic effect of Hordeum vulgare polysaccharide（HVP）on diabetes mellitus
model mice and its mechanism. METHODS：The mice were given intraperitoneal injection of streptozotocin（120 mg/kg）to induce
diabetes mellitus model. After modeling，the mice were randomly divided into model group，metformin group（positive control，
200 mg/kg），HVP high-dose，medium-dose and low-dose groups（300，150 and 75 mg/kg），with 10 mice in each group. Blank
control group was established additionally. Administration groups were given relevant medicine intragastrically；blank control group
and model group were given constant volume of water intragastrically，once a day，for consecutive 30 days. The levels of fasting
blood glucose（FBG）were determined after 10，20，30 days of administration. After last FBG test，mice were intraperitoneally
injected with 10％ glucose solution（2 g/kg），then the area under the glucose tolerance（GTT）curve was measured at 30 and 120
min after injection. The serum levels of insulin，the content of superoxide dismutase（SOD），glutathione peroxidase（GSH-Px），
malondialdehyde（MDA）in liver tissue were detected. Pancreatic morphology of mice were detected by HE staining. RESULTS：
Compared with blank control group，the FBG，area under GTT curve and MDA contnet in liver tissue were increased significantly
in model group（P＜0.01），while serum levels of insulin，SOD and GSH-Px contents in liver tissue were decreased significantly
（P＜0.01）；the pancreatic tissue was seriously damaged with incomplete morphology and obvious vacuoles. Compared with model
group，FBG，area under GTT curve，MDA content in liver
Δ 基金项目：国家自然科学基金资助项目（No.81473179） tissue were decreased significantly in HVP high-dose group
＊主管药师。研究方向：临床药学。电话：0510-85351490。E-
（P＜0.05 or P＜0.01），serum content of insulin，the content
mail：Wangshengya_helen@126.com
# 通信作者：副主任药师。研究方向：医院药学。电话：0510- of SOD and GSH-Px in liver tissue were increased
85351490。E-mail：3103221944@qq.com significantly （P＜0.05 or P＜0.01）；above indexes of HVP

中国药房 2021年第32卷第7期 China Pharmacy 2021 Vol. 32 No. 7 ·807 ·