Page 68 - 《中国药房》2021年第6期

湿润烧伤膏对脂多糖致大鼠皮肤成纤维细胞损伤的改善作用及

其机制研究 Δ

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姜 艳 1，2＊ ，巩奇明 ，韦 骋 ，陆飞燕 ，卓臣义 ，唐乾利 （1.广西大学医学院，南宁 530004；2.右江民族医学
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院科学实验中心，广西 百色 533000）
中图分类号 R285 文献标志码 A 文章编号 1001-0408（2021）06-0702-07
DOI 10.6039/j.issn.1001-0408.2021.06.10
摘 要 目的：研究湿润烧伤膏对脂多糖致大鼠皮肤成纤维细胞损伤的改善作用及其机制。方法：将大鼠皮肤成纤维细胞分为对
照组、脂多糖组（5 μg/mL）、康复新液组（阳性对照，5 μg/mL脂多糖+1.25％康复新液）和湿润烧伤膏组（5 μg/mL脂多糖+0.6 mg/mL
湿润烧伤膏），每组设置6个复孔。以脂多糖诱导建立炎性损伤细胞模型（对照组除外）。培养一定时间后，检测各组细胞存活率
和细胞迁移率，测定细胞上清液中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）含量，检测细胞内IL-6蛋白定位和荧光强度，检测
细胞中同源性磷酸酶张力蛋白（PTEN）、磷酸化p65（p-p65）、TNF-α、IL-6、磷脂酰肌醇3激酶（PI3K）和蛋白激酶B（Akt）蛋白的表
达情况。结果：与对照组比较，脂多糖组细胞存活率显著升高、迁移率显著降低，细胞上清液中TNF-α、IL-6含量和PTEN、p-p65、
TNF-α、IL-6和PI3K蛋白的相对表达量均显著升高（P＜0.01），IL-6蛋白主要在细胞质中表达且荧光强度增强。与脂多糖组比较，
康复新液组和湿润烧伤膏组细胞存活率均显著降低、迁移率均显著升高；TNF-α、IL-6含量和PTEN、p-p65、TNF-α、IL-6（除康复新
液组外）、PI3K、Akt（除康复新液组外）蛋白的相对表达量均显著降低（P＜0.05或P＜0.01），IL-6蛋白荧光强度减弱，且湿润烧伤膏
组细胞中TNF-α、IL-6、PI3K、Akt蛋白的相对表达量均显著低于康复新液组（P＜0.05或P＜0.01）。结论：湿润烧伤膏可抑制脂多
糖诱导的大鼠皮肤成纤维细胞增殖，降低炎性因子水平和炎症反应强度，其机制可能与下调PTEN/核因子κB、PI3K/Akt信号通路
有关。
关键词 湿润烧伤膏；皮肤成纤维细胞；炎症反应；同源性磷酸酶张力蛋白/核因子κB；磷脂酰肌醇3激酶/蛋白激酶B

Study on Improvement Effect and Mechanism of MEBO on LPS-induced Injury of Rat Skin Fibroblasts
JIANG Yan ，GONG Qiming ，WEI Cheng ，LU Feiyan ，ZHUO Chenyi ，TANG Qianli （1. Medical College，
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Guangxi University，Nanning 530004，China；2. Science Experiment Center， Youjiang Medical University for
Nationalities，Guangxi Baise 533000，China）
ABSTRACT OBJECTIVE：To study the improvement effect and mechanism of MEBO on lipopolysaccharide （LPS）-induced
injury of rat skin fibroblasts. METHODS：Skin fibroblasts of rats were divided into control group，LPS group （5 μ g/mL），
Kangfuxin solution group（positive control，5 μg/mL LPS+1.25％ Kangfuxin solution）and MEBO group（5 μg/mL LPS+0.6 mg/mL
MEBO），with 6 wells in each group. Inflammatory injury cell model was induced by LPS（except for control group）. After a
certain period of cultivation，the cell survival rate and cell migration rate were detected in each group. The contents of TNF-α and
IL-6 in cell supernatant was detected. The localization and fluorescence intensity of IL-6 protein were detected. The protein
expression of PTEN，p-p65，TNF-α ，IL-6，PI3K and Akt in the fibroblasts were also determined. RESULTS：Compared with
control group，survival rate of the fibroblasts was increased significantly in LPS group，while cell migration was decreased
significantly；the contents of TNF-α and IL-6 in cell supernatant as well as relative protein expression of PTEN，p-p65，TNF-α，
IL-6 and PI3K were increased significantly （P＜0.05 or P＜0.01）；IL-6 protein mainly expressed in the cytoplasm，and the
fluorescence intensity was enhanced. Compared with LPS group，survival rate of the fibroblasts was decreased significantly in
Kangfuxin solution group and MEBO group，while migration rate was increased significantly；the contents of TNF-α and IL-6，
relative protein expression of PTEN，p-p65，TNF-α ，IL-6 （except for Kangfuxin solution group），PI3K and Akt （except for
Kangfuxin solution group） were decreased significantly （P＜0.05 or P＜0.01），while fluorescence intensity of IL-6 protein
decreased；relative protein expression of TNF-α，IL-6，PI3K and Akt in MEBO group were significantly lower than Kangfuxin
solution group （P＜0.05 or P＜0.01）. CONCLUSIONS：MEBO can inhibit the proliferation of LPS-induced skin fibroblasts，
reduce the level of inflammatory factors and the intensity of
Δ 基金项目：国家自然科学基金面上项目（No.81774327）；广西自
inflammatory reaction， which may be related to the
治区特聘专家专项经费资助（No.桂人才通字〔2019〕13号）
＊实验师，博士研究生。研究方向：细胞生物学。E-mail：jiang- down-regulation of PTEN/NF-κB，PI3K/Akt signaling pathway.
yan9hr@yeah.net KEYWORDS MEBO； Skin fibroblasts； Inflammation
# 通信作者：教授，博士生导师，博士。研究方向：创面修复机制 reaction；PTEN/NF-κB；PI3K/Akt
研究。E-mail：htmgx@163.com

·702 · China Pharmacy 2021 Vol. 32 No. 6 中国药房 2021年第32卷第6期