Page 48 - 《中国药房》2021年第6期

双歧杆菌联合左卡尼汀对菌群失调腹泻模型大鼠肠道菌群的影

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王重娟 ，周锦妍 ，王崇静 ，梁月琴 ，朱瑜丹 ，王星星 ，李仲昆 （1.昆明医科大学附属延安医院药学部，昆明
650051；2.昆明医科大学附属延安医院儿科，昆明 650051；3.云南新兴职业学院药学院，昆明 650501）
中图分类号 R963；R372 文献标志码 A 文章编号 1001-0408（2021）06-0682-09
DOI 10.6039/j.issn.1001-0408.2021.06.07
摘 要 目的：研究双歧杆菌联合左卡尼汀对菌群失调腹泻模型大鼠肠道菌群的影响。方法：将30只SD大鼠随机分为空白对照
组、模型组、益生菌组（双歧杆菌三联活菌肠溶胶囊 70 mg/mL）、左卡尼汀组（左卡尼汀注射液 50 mg/mL）和左卡尼汀+益生菌组
（左卡尼汀注射液50 mg/mL+双歧杆菌三联活菌肠溶胶囊70 mg/mL）。除空白对照组外，其余各组大鼠均连续灌胃50 mg/mL克
林霉素磷酸酯（2 mL/只，每天1次，连续4天）以建立菌群失调腹泻模型。实验第5天起进入恢复期，各给药组大鼠开始灌胃相应
药物，空白对照组和模型组大鼠灌胃等体积生理盐水；灌胃体积均为1 mL/只，每天1次，连续给药7天。实验期间观察各组大鼠的
一般情况；收集造模期结束时正常对照组和模型组大鼠的粪便以及恢复期末次给药后各组大鼠的粪便，分别进行肠道菌群基因组
DNA提取与聚合酶链式反应扩增、文库构建和高通量测序，并对处理后的有效数据进行操作分类单元聚类、物种注释以及肠道菌
群的Alpha和Beta多样性分析。结果：造模期结束时，与空白对照组比较，模型组大鼠开始出现1级和2级粪便，肠道菌群的多样
性、丰富度以及肠道中厚壁菌门/拟杆菌门比值和乳杆菌属、双歧杆菌属和阿克曼氏菌属等益生菌的丰度均显著降低（P＜0.05），
而肠球菌属等致病菌的丰度显著升高（P＜0.05）。恢复期结束时，与模型组比较，益生菌组、左卡尼汀组和左卡尼汀+益生菌组大
鼠的活动量和粪便的形态、颜色恢复至正常，肠道菌群的多样性和丰富度差异均无统计学意义（P＞0.05），但其肠道中乳杆菌属的
丰度有一定提高，且左卡尼汀+益生菌组大鼠肠道中阿克曼氏菌属的丰度显著升高（P＜0.05）。结论：双歧杆菌联合左卡尼汀虽对
提高菌群失调腹泻模型大鼠肠道菌群的多样性和丰富度无显著效果，但能在一定程度上增加其肠道中益生菌的丰度。
关键词 肠道菌群；益生菌；左卡尼汀；双歧杆菌；高通量测序；多样性

Effects of Bifidobacterium Combined with L-carnitine on Intestinal Flora of Dysbacteriosis Diarrhea Model
Rats
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WANG Chongjuan ，ZHOU Jinyan ，WANG Chongjing ，LIANG Yueqin ，ZHU Yudan ，WANG Xingxing ，
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LI Zhongkun（1. Dept. of Pharmacy，the Affiliated Yan’an Hospital of Kunming Medical University，Kunming
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650051，China；2. Dept. of Pediatrics，the Affiliated Yan’an Hospital of Kunming Medical University，
Kunming 650051，China；3. School of Pharmacy，Yunnan Xinxing Vocational College，Kunming 650501，
China）
ABSTRACT OBJECTIVE：To study the effects of Bifidobacterium combined with L-carnitine on intestinal flora of dysbacteriosis
diarrhea model rats. METHODS：Totally 30 SD rats were randomly divided into blank control group，model group，probiotics
group（Bifidobacterium triple viable enteric coated capsules 70 mg/mL），L-carnitine group（L-carnitine injection 50 mg/mL）and
L-carnitine + probiotics group（L-carnitine injection 50 mg/mL + Bifidobacterium triple viable enteric coated capsules 70 mg/mL）.
Except for blank control group，the rats in other groups were given 50 mg/mL clindamycin phosphate intragastrically（2 mL/rat，
once a day，for 4 consecutive days）to establish the model of dysbacteriosis diarrhea. On the 5th day of the experiment，the rats in
administration groups were given corresponding drugs intragastrically，blank control group and model group were given equal
volume of normal saline intragastrically；with the dosage volume of 1 mL/rat，once a day，for consecutive 7 days. The general
situation of rats in each group was observed during the experiment. The feces of normal control group and model group at the end
of the modeling and the feces of the rats in administration group after the last administration were collected for genomic DNA
extraction，polymerase chain reaction amplification，library construction and high-throughput sequencing. After processing，the
effective data were analyzed by operational taxonomic unitsclustering and species annotation，as well as Alpha and Beta diversity of
intestinal flora. RESULTS： At the end of the modeling，
Δ 基金项目：国家自然科学基金资助项目（No.82060744）；云南省
compared with blank control group，grade 1 feces and grade 2
高层次卫生技术人才培养专项经费（No.L-201625）；云南省科技厅-昆
明医科大学基础研究联合专项[No.2018FE001（-086）] feces were found in model group. The diversity and richness
＊主管药师，硕士。研究方向：药理学及分子生物学。E-mail： of intestinal flora，the ratio of Firmicutes/Bacteroidetes and
zhongjuanwang7@163.com the abundance of probiotics such as Lactobacillus，
# 通信作者：主任药师，硕士生导师。研究方向：药理学及医院药 Bifidobacterium and Ackermann were significantly decreased
学。E-mail：yayylzk@163.com （P＜0.05），while the abundance of pathogenic bacteria such

·682 · China Pharmacy 2021 Vol. 32 No. 6 中国药房 2021年第32卷第6期