Page 36 - 《中国药房》2021年5期

UPLC法测定芭蕉药材不同部位中羽扇豆酮和豆甾醇的含量 Δ

吴红梅 ，孔 娟 ，黄旭龙 ，杨小松 ，王祥培 （1.贵州中医药大学药学院，贵阳 550025；2.贵州民族大学民族
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医药学院，贵阳 550025）
中图分类号 R284.1；R927.2 文献标志码 A 文章编号 1001-0408（2021）05-0542-05
DOI 10.6039/j.issn.1001-0408.2021.05.06

摘 要 目的：建立检测同植株芭蕉根茎、茎和叶中羽扇豆酮和豆甾醇含量的方法，并为寻找芭蕉根茎有效成分的替代资源提供
依据。方法：采用超高效液相色谱（UPLC）法进行测定，色谱柱为Zorbax Rrhd Eclipse Plus C18 （100 mm×2.1 mm，1.8 μm），流动相
为乙腈-甲醇（78.5 ∶ 21.5，V/V），检测波长为210 nm，流速为0.15 mL/min，柱温为30 ℃，进样量为1 μL。对9批同植株芭蕉根茎、茎
和叶中羽扇豆酮和豆甾醇的含量测定结果进行组间差异性分析、主成分分析和聚类分析。结果：羽扇豆酮和豆甾醇的质量浓度分
别在11.16～357.10、8.83～160.40 µg/mL范围内与各自峰面积呈良好的线性关系（R 分别为0.999 2、0.999 1）；精密度、重复性、稳
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定性试验的RSD均小于3％；平均加样回收率分别为101.44％、98.32％，RSD分别为1.77％、1.81％（n＝6）。芭蕉茎中羽扇豆酮和
豆甾醇的平均含量显著高于根茎和叶（P＜0.05）；同植株芭蕉根茎与叶中羽扇豆酮和豆甾醇的含量比较，差异无统计学意义（P＞
0.05）。主成分分析结果表明，同植株芭蕉根茎、茎、叶中羽扇豆酮和豆甾醇的含量具有差异。聚类分析将芭蕉根茎、茎和叶分为3
大类，其中以芭蕉茎与其余2个部位的差异较明显。结论：该方法简单、快捷、专属性强、重复性好、准确性高，可用于芭蕉药材不
同部位中羽扇豆酮和豆甾醇的含量测定；芭蕉茎可替代芭蕉根茎作为羽扇豆酮和豆甾醇的原药材来源。
关键词 芭蕉；药材部位；超高效液相色谱法；含量测定；主成分分析；聚类分析

Content Determination of Lupanone and Stigmasterol in Different Parts of Musa basjoo by UPLC
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WU Hongmei ，KONG Juan ，HUANG Xulong ，YANG Xiaosong ，WANG Xiangpei（1.College of Pharmacy，
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Guizhou University of TCM， Guiyang 550025， China； 2. College of Ethnic Medicine， Guizhou Minzu
University，Guiyang 550025，China）
ABSTRACT OBJECTIVE：To establish a method for determining the contents of lupenone and stigmasterol in the rhizome，stem
and leaf of Mosa basjoo from the same plant，and to provide reference for the substitute resource for the effective components of
M. basjoo. METHODS：UPLC method was adopted. The determination was performed on Zorbax Rrhd Eclipse Plus C18 column
（100 mm×2.1 mm，1.8 μm）with mobile phase consisted of acetonitrile-methanol（78.5 ∶ 21.5，V/V）. The detection wavelength was
set at 210 nm；the flow rate was 0.15 mL/min；the column temperature was 30 ℃ and the sample size was 1 μL. The results of
content determination of lupinone and stigmasterol in the rhizome，stem and leaf of 9 batches of M. basjoo from the same plant
were analyzed by the methods of comparative analysis between groups，principal component analysis and cluster analysis.
RESULTS：The mass concentration of lupenone and stigmasterol had a good linear relationship with the corresponding peak area
within the range of 11.16-357.10 and 8.83-160.40 g/mL（R were 0.999 2 and 0.999 1，respectively）. RSDs of precision，
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repeatability and stability tests were all less than 3％. The average recovery rates of lupenone and stigmasterol were 101.44％ and
98.32％，and the RSDs were 1.77％ and 1.81％（n＝6），respectively. The average contents of lupenone and stigmasterol in stems
of M. Basjoo were significantly higher than those of rhizome and leaves of M. basjoo （P＜0.05） . There was no statistical
significance in the contents of lupenone and stigmasterol between stem and leaf of M. basjoo from same plant（P＞0.05）. Results
of principal component analysis showed that the contents of lupanone and stigmasterol were different in rhizome，stem and leaf of
M. basjoo from the same plant. Rhizome，stem and leaf of M. basjoo were divided into three types through cluster analysis，among
which the rhizome had significant difference with the other two parts. CONCLUSIONS：The method is simple，rapid，specific，
reproducible and accurate. It can be used for the content determination of lupenone and stigmasterol in different parts of M. basjoo.
The stem of M. basjoo can replace the rhizome of M. basjoo as the source of lupinone and stigmasterol.
KEYWORDS Mosa basjoo；Medicinal part；UPLC；Content determination；Principal component analysis；Cluster analysis
Δ 基 金 项 目 ：国 家 自 然 科 学 基 金 资 助 项 目（No.81860737，
No.81860722）
芭 蕉 根 为 芭 蕉 科 植 物 芭 蕉 Musa basjoo Sied. et
＊副教授，硕士生导师，博士。研究方向：中药、民族药质量控制
Zucc.的干燥根茎，是贵州苗族地区习用苗药，收载于
及新药研发。E-mail：381176659@qq.com
# 通信作者：教授，硕士生导师，博士。研究方向：中药（民族药） 2003 年版《贵州省中药材、民族药材质量标准》，具有清
[1]
品种品质与资源开发。E-mail：709521748@qq.com 热解毒、止渴利尿的功效 。现代研究发现，芭蕉根具有

·542 · China Pharmacy 2021 Vol. 32 No. 5 中国药房 2021年第32卷第5期