Page 30 - 《中国药房》2021年5期

·药学研究·

蒙药哈日-沙布嘎（铁杆蒿）的质量标准提升 Δ

袁小红 ，庞克坚 ，唐 辉 ，张 虹 ，魏 沣 ，刘乐乐 ，姜国珍（1.石河子大学药学院/新疆植物药资源利用教
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育部重点实验室，新疆 石河子 832000；2.和田维吾尔药业股份有限公司，新疆 和田 848200；3.博尔塔拉蒙古
自治州蒙医医院，新疆 博乐 833400）
中图分类号 R284.1 文献标志码 A 文章编号 1001-0408（2021）05-0536-06
DOI 10.6039/j.issn.1001-0408.2021.05.05
摘 要 目的：提升蒙药哈日-沙布嘎（铁杆蒿）的质量标准，为全面评价其质量提供科学依据。方法：对哈日-沙布嘎进行外观性
状鉴别和显微鉴别；采用薄层色谱（TLC）法对药材中的东莨菪苷、绿原酸、咖啡酸、东莨菪内酯和3，5-二咖啡酰奎宁酸进行定性鉴
别；建立高效液相色谱（HPLC）法测定药材中上述5种成分的含量；并对药材的水分、总灰分和浸出物进行检查。结果：哈日-沙布
嘎药材茎呈类圆柱形，表面呈紫色或紫褐色或青褐色；叶呈卵形或矩圆状卵形，气香；花黄色，头状花序，近球形或半球形；粉末呈
绿色或黄绿色，花粉粒具3个萌发孔，薄壁细胞簇晶棱尖锐，螺纹导管众多，偶见具缘纹孔导管，木纤维多成束存在。TLC结果显
示，供试品在与5种对照品色谱相应位置上均显相同颜色的斑点。东莨菪苷、绿原酸、咖啡酸、东莨菪内酯、3，5-二咖啡酰奎宁酸的
质量浓度分别在 85.60～428.00、10.16～101.60、10.20～102.00、40.84～408.40、40.80～408.00 μg/mL 范围内与各自峰面积呈良好
线性关系（r均大于0.999 0）；精密度、稳定性、重复性试验的RSD均小于3.00％（n＝6）；平均加样回收率分别为103.07％、99.66％、
98.37％、97.78％、98.40％（RSD 均小于 3.00％，n＝6）。10 批药材样品中上述 5 种成分含量范围分别为 0.36％～1.23％、0.09％～
0.51％、0.04％～0.13％、0.61％～1.13％、0.12％～1.11％；水分、总灰分、水溶性浸出物的平均含量分别为 6.25％、5.86％、
26.50％。结论：该研究在哈日-沙布嘎原质量标准的基础上增加了显微鉴别、TLC鉴别、含量测定以及水分、总灰分和浸出物等检
查项，方法精密、准确、稳定性良好，能够为更加科学、规范地评价该药材质量提供依据。
关键词 蒙药；哈日-沙布嘎（铁杆蒿）；质量标准；显微鉴别；薄层色谱法；高效液相色谱法；东莨菪苷；绿原酸；咖啡酸；东莨菪内
酯；3，5-二咖啡酰奎宁酸

Improvement of Quality Standard for Mongolian Medicine Artemisia sacrorum
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YUAN Xiaohong ，PANG Kejian ，TANG Hui ，ZHANG Hong ，WEI Feng ，LIU Lele ，JIANG Guozhen（1.
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Pharmacy School of Shihezi University/Key Laboratory of the Ministry of Education of Xinjiang Special Plant
Medicinal Resources，Xinjiang Shihezi 832000，China；2. Hetian Uygur Pharmaceutical Co.，Ltd.，Xinjiang
Hetian 848200，China；3. Mongolian Medical Hospital of Bortala Mongolian Autonomous Prefecture，Xinjiang
Bole 833400，China）
ABSTRACT OBJECTIVE：To improve the quality standard of Mongolian medicine Artemisia sacrorum，and to provide scientific
basis for comprehensive quality evaluation. METHODS：The appearance and microscopic characteristics of A. sacrorum were
identified；scopoletin，chlorogenic acid，caffeic acid，scopoletin and 3，5-dicaffeoylquinic acid were identified quantitatively by
TLC；the contents of above 5 components were determined by HPLC. The water content，total ash and extract were examined.
RESULTS：The stem of A. sacrorum was cylindrical，and its surface was purple or purple-brown or cyan-brown；the leaves were
ovate or oblong-ovate，fragrant；the flowers were yellow，head-shaped，subglobose or hemispherical. The powder was green or
yellow-green，its pollen grain had three germination；the parenchymal cell clusters with sharp edges and numerous threaded ducts，
occasionally having marginal pitted ducts；its wood fibers were in bundles mostly. Results of TLC showed that the spots of the
same color were found in the corresponding positions of chromatogram for 5 substance control and samples. The linear range of
scopoletin， chlorogenic acid， caffeic acid， scopolactone and 3，5-dicaffeoylquinic acid were 85.60-428.00， 10.16-101.60，
10.20-102.00，40.84-408.40 and 40.80-408.00 μg/mL（all r＞0.999 0）. RSDs of precision，stability，repeatability tests were all less
than 3.00％（n＝6）. The average recoveries were 103.07％，99.66％，98.37％，97.78％，98.40％（all RSDs＜3.00％，n＝6）. The
contents of the above-mentioned 5 compounds in 10 batches of
Δ 基金项目：国家自然科学基金资助项目（No.81860614）；新疆
samples were 0.36％-1.23％，0.09％-0.51％，0.04％-0.13％，
维吾尔自治区重点研发计划项目（No.2017B03013-1）
＊硕士研究生。研究方向：药物分析。E-mail：1120448744@qq. 0.61％ -1.13％ ， 0.12％ -1.11％ ， respectively； the average
com contents of water，total ash and water soluble extract were
# 通信作者：教授，硕士生导师，博士。研究方向：药物分析。电 6.25％，5.86％，26.50％，respectively. CONCLUSIONS：On
话：0993-2057878。E-mail：Tanghuishz@qq.com the basis of the original quality standard of A. sacrorum，

·536 · China Pharmacy 2021 Vol. 32 No. 5 中国药房 2021年第32卷第5期