Page 82 - 《中国药房》2021年第4期

ABSTRACT OBJECTIVE：To study the anti-inflammatory effects of Yuyang capsule on bacterial dermatitis model rats and its
effect on TLR/NF-κ B pathway. METHODS：Minimum inhibition concentration （MIC） and minimal bactericidal concentration
（MBC）of Yuyang capsule against Staphylococcus aureus were determined by microdilution test. Totally 50 SD rats were randomly
divided into model group，positive control group （amoxicillin and clavulanate potassium，120 mg/kg as amoxicillin），Yuyang
capsules high-dose，medium-dose and low-dose groups（according to MIC），with 10 rats in each group. The model of bacterial
dermatitis was established by using the burned skin of rats infected with S. aureus. 24 h after modeling，administration groups were
intragastrically given the corresponding drug，and model group was intragastrically given the same amount of normal saline，once a
day，for consecutive 7 days. The skin healing rate was calculated on the 1st，3rd，5th and 7th day of administration，and the scab
formation，decrustation and healing were recorded. The contents of IL-1β，IL-6，IL-10，TNF-α，hydroxyproline（HYP），collagen
Ⅰ（Col Ⅰ）and Col Ⅲ in skin tissue were detected by ELISA. Morphology changes of skin tissues were observed by HE staining.
The ultrastructure was observed by transmission electron microscope. Protein expression of TLR2，TLR4 and p-NF-κB p65 were
detected by Western blotting assay. RESULTS：MIC and MBC of Yuyang capsule were 25 mg/mL and 50 mg/mL，respectively.
Dose of Yuyang capsules high-dose，medium-dose and low-dose groups were set at 600，300，150 mg/kg. Compared with model
group，scab appeared on the injured skin 3 days after administration in Yuyang capsule high-dose and medium-dose groups，and
decrustation appeared on the injured skin of part mice 5-7 days after administration；the skin healing rate of the positive control
group，Yuyang capsule high-dose and medium-dose groups were all significantly increased at each time point. The contents of IL-1
β，IL-6，IL-10 and TNF-α，pathological score，protein expression of TLR2，TLR4 and p-NF-κB p65 were decreased significantly
（P＜0.05 or P＜0.01）；the pathological changes such as inflammatory cell infiltration in skin tissue were improved. The contents of
HYP，Col Ⅰ and Col Ⅲ were increased significantly in positive control group and Yuyang capsule high-dose group（P＜0.05 or
P＜0.01）. There was no statistical significance in most above indexes in positive control group，Yuyang capsule high-dose and
medium-dose groups（P＞0.05）. CONCLUSIONS：Yuyang capsule can promote skin healing of bacterial dermatitis model rats and
shows certain anti-inflammatory effects；the mechanism may be related to inhibiting TLR/NF-κB signaling pathway and reducing
inflammatory response.
KEYWORDS Yuyang capsules；Bacterial infection；Infectious dermatitis；TLR/NF-κ B signaling pathway；Antibacterial and
anti-inflammatory；Rat

金黄色葡萄球菌 Staphylococcus aureus 是一种革兰 也是创面皮肤伤口愈合缓慢的原因之一 [6－7] 。基于此，
氏阳性菌，常在人前鼻孔和皮肤中定植，是绝大多数皮 本文研究了愈疡胶囊对细菌感染皮炎模型大鼠的抗炎
[1]
肤和软组织感染的主要病原体 。金黄色葡萄球菌可导 作用及对TLR/NF-κB通路的影响，旨在为扩大愈疡胶囊
致多种感染，是造成慢性创面皮肤伤口感染的主要原因 的临床应用、促进相关药物研发提供实验依据。
[2]
之一，也是引起术后感染性并发症的关键 。目前临床 1 材料
治疗金黄色葡萄球菌感染主要以抗生素为主，但存在细 1.1 主要仪器
菌耐药的风险 。因此，寻找新的有效的抗菌制剂就变 本研究所用主要仪器有：SpectraMAX Plus384 型酶
[3]
得十分重要。 标仪[美谷分子仪器（上海）有限公司]、PHY-Ⅲ型病理组
愈疡胶囊是凉山彝族自治州第二人民医院独具特 织漂烘仪（常州市中威电子仪器有限公司）、DHP-9012
色的院内制剂之一，其主要成分为山药与蜂蜡，主要功 型电热细菌培养箱（上海一恒科学仪器有限公司）、
效为生肌、敛疮、散结、止痛，临床多用于治疗溃疡性口 BA400 Digital型数码三目摄像显微镜（麦克奥迪实业集
腔炎、乳腺增生、卵巢囊肿等症。临床实践显示，在60例 团有限公司）、JEM-1400 PLUS型透射电子显微镜（日本
[4]
卵巢囊肿患者中，愈疡胶囊治疗的总有效率达到90％ ； JEOL 公司）、优普超纯水制造系统（成都超纯科技有限
药效学与毒理学实验表明，该药能通过调节大鼠体内激 公司）、DZKW-4型电子恒温水浴锅（北京中兴伟业仪器
素分泌水平来改善乳腺增生，且长期用药未见明显毒性 有限公司）、JY200C型电泳仪和JY-SCZ4+型垂直电泳槽
[5]
反应 。根据我院临床治疗经验与笔者前期预实验结果 （北京君意东方电泳设备有限公司）、5200型化学发光凝
表明，愈疡胶囊具有抗菌消炎等作用，但该制剂发挥上 胶成像仪（上海天能科技有限公司）、THZ-98A恒温振荡
述作用的具体机制尚不明确。 器（上海一恒科学仪器有限公司）。
Toll 样受体/核因子κB（TLR/NF-κB）通路是经典的 1.2 主要药品与试剂
炎症通路，细菌等病原微生物感染可导致该通路的激 愈疡胶囊[批号190901，川药制字Z200800943，规格
活，诱导炎症因子的释放，从而促进炎症反应的发生，这 0.45 g/粒（1 g药粉相当于原生药1.5 g）]由凉山彝族自治

·460 · China Pharmacy 2021 Vol. 32 No. 4 中国药房 2021年第32卷第4期