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滇黄芩茎叶乙醇提取物及其不同溶剂萃取部位的抗氧化和降脂

活性研究 Δ

李欣坪 ，王蒙蒙，王子晨，方琼莲，林玉萍（云南中医药大学中药学院/云南省南药可持续利用重点实验室，昆明
＊
#
650500）

中图分类号 R285 文献标志码 A 文章编号 1001-0408（2021）02-0220-06
DOI 10.6039/j.issn.1001-0408.2021.02.16
摘 要 目的：研究滇黄芩茎叶乙醇提取物及其不同溶剂萃取部位的抗氧化活性和降脂活性。方法：取滇黄芩茎叶用95％乙醇
回流提取，得乙醇提取物；取上述提取物，依次用石油醚、乙酸乙酯、正丁醇萃取，回收溶剂得到不同溶剂萃取部位。以维生素C
（Vc）为阳性对照，采用羟基自由基、超氧阴离子自由基、1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除法检测滇黄芩茎叶乙醇提
取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物的体外抗氧化活性并计算半数抑制浓度（IC50 ）。以脂肪乳建立脂肪变性L02
肝细胞模型，以非诺贝特（20 μg/mL）为阳性对照，考察高、低质量浓度（100、50 μg/mL）滇黄芩茎叶乙醇提取物、乙酸乙酯萃取物、
正丁醇萃取物对细胞中TC、TG含量的影响。结果：对羟基自由基的清除能力强弱排序为滇黄芩茎叶正丁醇萃取物＞乙酸乙酯萃
取物＞Vc＞乙醇提取物＞石油醚萃取物，IC50分别为0.15、0.17、0.35、0.75、1.17 mg/mL；对超氧阴离子自由基的清除能力强弱排序
为Vc＞正丁醇萃取物＞乙酸乙酯萃取物＞乙醇提取物＞石油醚萃取物，IC50分别为0.034、0.55、0.75、3.32、3.73 mg/mL；对DPPH
自由基的清除能力强弱排序为 Vc＞正丁醇萃取物＞乙酸乙酯萃取物＞乙醇提取物＞石油醚萃取物，IC50分别为 0.003 2、0.028、
0.033、0.048、0.057 mg/mL。滇黄芩茎叶乙醇提取物、乙酸乙酯萃取物和正丁醇萃取物对脂肪变性L02肝细胞中TC、TG含量均有
显著的降低作用（P＜0.01），其降脂能力强弱排序为正丁醇萃取物（低浓度）≈非诺贝特＞乙酸乙酯萃取物（高浓度）＞乙醇提取物
（高浓度）＞正丁醇萃取物（高浓度）＞乙酸乙酯萃取物（低浓度）＞乙醇提取物（低浓度）。结论：滇黄芩茎叶乙醇提取物、石油醚萃
取物、乙酸乙酯萃取物、正丁醇萃取物均有一定的抗氧化活性和降脂活性（除石油醚萃取物外），其中正丁醇和乙酸乙酯萃取物的
抗氧化活性和降脂活性较高。
关键词 滇黄芩茎叶；乙醇提取物；抗氧化活性；降脂活性

Study on Antioxidant Activity and Lipid-lowering Effect of Ethanol Extract and Its Different Solvent
Extracts from the Stem and Leaves of Scutellaria amoena
LI Xinping，WANG Mengmeng，WANG Zichen，FANG Qionglian，LIN Yuping（School of Chinese Materia
Medica and Yunnan Key Laboratory of Southern Medicinal Resource，Yunnan University of Chinese Medicine，
Kunming 650500，China）

ABSTRACT OBJECTIVE：To study the antioxidant activity and lipid-lowering effect of ethanol extract and its different solvent
extracts from the stems and leaves of Scutellaria amoena. METHODS：The stem and leaves of S. amoena was extracted with 95％
ethanol to obtain ethanol extract，and then extracted with petroleum，ethyl acetate and n-butanol to obtain corresponding different
solvent extracts. Using vitamin C（Vc）as positive control，the antioxidant activities of ethanol extract，petroleum ether extract，
ethyl acetate extract and n-butanol extract from the stems and leaves of S. amoena were determined by hydroxyl radical，superoxide
anion radical and DPPH radical scavenging method，and the IC50 was calculated. Steatosis L02 hepatocyte model was established
with fat emulsion. Using fenofibrate（20 μg/mL）as positive control，the effects of high and low concentration（100 and 50 μg/mL）
ethanol extract，ethyl acetate extract and n-butanol extract from the stems and leaves of S. amoena on the contents of TC and TG in
cells were investigated. RESULTS：The order of scavenging ability to hydroxyl radicals was n-butanol extract＞ethyl acetate
extract＞Vc＞ethanol extract＞petroleum ether extract；IC50 of them were 0.15，0.17，0.35，0.75，1.17 mg/mL，respectively. The
order of scavenging ability to superoxide anion radical was Vc＞n-butanol extract＞ethyl acetate extract＞ethanol extract＞
petroleum ether extract；IC50 of them were 0.034，0.55，0.75，3.32，3.73 mg/mL，respectively. The order of DPPH scavenging
ability to DPPH radical was Vc＞n-butanol extract＞ethyl
Δ 基金项目：云南省科技厅云南中医学院应用基础研究联合专项
acetate extract＞ethanol extract＞petroleum ether extract；IC50
[No.2017F117（-022）]；云南中医药大学大学生创新、创业训练项目
of them were 0.003 2，0.028，0.033，0.048，0.057 mg/mL，
（No.2019）
respectively. The ethanol extract， ethyl acetate extract and
＊硕士研究生。研究方向：中药资源利用与开发。电话：0871-
67429034。E-mail：2515034118@qq.com n-butanol extract from the stems and leaves of S. amoena
# 通信作者：副教授，硕士生导师。研究方向：中药、天然药物活 could significantly decrease the contents of TC and TG in
性。电话：0871-67429034。E-mail：11217005@qq.com steatosis L02 hepatocytes （P＜0.01）. The order of

·220 · China Pharmacy 2021 Vol. 32 No. 2 中国药房 2021年第32卷第2期