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栀子和淡豆豉不同比例配伍对栀子豉汤中黄酮类成分含量的影

响 Δ

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李函阳 ，邱智东，苏文龙，曹文正，李昕曈，蒋常鹏，高红梅（长春中医药大学药学院，长春 130117）
＊
中图分类号 R283；R284 文献标志码 A 文章编号 1001-0408（2020）09-1103-05
DOI 10.6039/j.issn.1001-0408.2020.09.15

摘 要 目的：考察栀子与淡豆豉以不同比例配伍后对栀子豉汤中染料木素和总黄酮含量的影响，进一步探究栀子豉汤的配伍规
律。方法：采用煎煮法分别制备栀子与淡豆豉不同配伍比例（2 ∶ 1、1 ∶ 1、1 ∶ 2、1 ∶ 4，质量比，下同）的栀子豉汤以及相应比例栀子、淡
豆豉的单煎液。采用超高效液相色谱-串联质谱（UPLC-MS/MS）法测定不同配伍比例栀子豉汤以及相应比例淡豆豉单煎液中染
料木素的含量；采用紫外分光光度（UV）法测定不同配伍比例栀子豉汤以及相应比例栀子单煎液、淡豆豉单煎液中总黄酮的含
量。结果：建立的含量测定方法的线性关系、精密度、重复性、稳定性、准确度均良好。栀子与淡豆豉不同配伍比例（2∶1、1∶1、1∶2、
1 ∶ 4）的栀子豉汤中染料木素含量较相应单煎液均有不同程度的降低，而总黄酮含量均有不同程度的升高。随着淡豆豉用量的增
加，栀子豉汤中染料木素含量呈现先升高后降低的趋势，当栀子与淡豆豉的配伍比例为1 ∶ 1、1 ∶ 2时，栀子豉汤中染料木素含量最
高（均为0.071 µg/mL）。随着淡豆豉用量的增加，栀子豉汤中总黄酮含量变化无明显规律，但在栀子与淡豆豉的配伍比例为1 ∶ 1
时栀子豉汤中总黄酮含量最高（1.861 µg/mL）。结论：当栀子与淡豆豉按1∶1的比例配伍时栀子豉汤中黄酮类成分的含量最高。
关键词 栀子豉汤；淡豆豉；配伍规律；染料木素；总黄酮；含量测定

Effect of Different Compatibility Ratio of Gardenia jasminoides to Fermented Soybean on the Content of
Flavonoids in Zhizichi Decoction
LI Hanyang，QIU Zhidong，SU Wenlong，CAO Wenzheng，LI Xintong，JIANG Changpeng，GAO Hongmei
（College of Pharmacy，Changchun University of TCM，Changchun 130117，China）

ABSTRACT OBJECTIVE：To investigate the effects of different compatibility ratio of Gardenia jasminoides to fermented
soybean on the content of genistein and total flavonoids，and to investigate the compatibility regularity of Zhizichi decoction.
METHODS：The decoction method was used to prepare the mixed decoction with different compatibility ratio of G. jasminoides to
fermented soybean（2 ∶ 1，1 ∶ 1，1 ∶ 2，1 ∶ 4，m/m，the same hereinafter）. UPLC-MS/MS method was used to determine the content of
genistein in Zhizichi decoction with different compatibility ratio and corresponding fermented soybean single decoction. UV method
was used to determine the content of total flavonoids in Zhizichi decoction with different compatibility ratio and corresponding
gardenia single decoction and fermented soybean single decoction. RESULTS：The established method had good linearity，
precision，repeatability，stability and accuracy. Compared with single decoction，the content of genistein in the mixed decoction
with different compatibility ratio of G. jasminoides to fermented soybean（2 ∶ 1，1 ∶ 1，1 ∶ 2，1 ∶ 4）was decreased to different extents，
while the content of total flavonoids was increased to different extents. With the increase of fermented soybean，the content of
genistein in the decoction increased at first and then decreased. When the compatibility ratios of G. jasminoides to fermented
soybean were 1 ∶ 1 and 1 ∶ 2，the content of genistein in the decoction was the highest（all 0.071 μg/mL）. With the increase of
fermented soybean，the content of total flavonoids in the decoction did not change regularly；when the ratio of G. jasminoides to
fermented soybean was 1 ∶ 1，the content of total flavonoids in the decoction was the highest（1.861 μg/mL）. CONCLUSIONS：
When the compatibility ratio of G. jasminoides to fermented soybean was 1 ∶ 1，the content of flavonoids in the decoction is the
highest.
KEYWORDS Zhizichi decoction； Fermented soybean； Compatibility regularity； Genistein； Total flavonoids； Content
determination

栀子豉汤出自汉代医圣张仲景之《伤寒论》中太阳、 经 。栀子可清热泻火，与能宣散邪热的淡豆豉配伍后，
[2]
阳明篇，由栀子和淡豆豉两味药组成 。方中栀子性寒， 可作为治疗热扰胸膈证和阳明郁热证的基础方 。现代
[1]
[3]
味苦，归心、肺、三焦经；淡豆豉性凉，味苦、辛，归肺、胃 临床中，栀子豉汤及其加减方可用于治疗不寐、焦虑、郁
[4]
Δ 基金项目：吉林省科技发展计划项目（No.20190304091YY）；吉 证、漏汗等疾病 ，疗效显著。栀子中主要含有环烯醚萜
林省中医药科技项目（No.2018D208）；长春中医药大学培育基金项目 类、黄酮类成分，其中环烯醚萜类成分具有抗抑郁作用 ；
[5]
＊硕士研究生。研究方向：药物新剂型与新技术。E-mail：heath-
淡豆豉中主要含黄酮类成分，包括大豆素、黄豆素、染料
erlii@163.com [5]
# 通信作者：副教授，博士。研究方向：中药炮制。E-mail：gh- 木素、黄豆苷、染料木苷等 ，具有抗癌、预防心血管疾
[6]
mice_2010@126.com 病、抗骨质疏松等药理作用 。据文献报道，黄酮类化合

中国药房 2020年第31卷第9期 China Pharmacy 2020 Vol. 31 No. 9 ·1103 ·