Page 58 - 2019年10月第30卷第19期

三脉菝葜醇提物及其不同极性萃取部位的抗肿瘤作用研究 Δ

1 #
鲁 遇 1，2＊ ，余英才 ，梁永红 （1.江西中医药大学现代中药制剂教育部重点实验室/创新药物与高效节能降耗制
2
药设备国家重点实验室，南昌 330004；2.江西中医药大学生物化学教研室，南昌 330004）

中图分类号 R285.5 文献标志码 A 文章编号 1001-0408（2019）19-2645-05
DOI 10.6039/j.issn.1001-0408.2019.19.11

摘 要 目的：研究三脉菝葜醇提物及其不同极性萃取部位的抗肿瘤作用，为其抗肿瘤活性部位的筛选提供参考。方法：采用
MTT法检测不同质量浓度的三脉菝葜醇提物及其石油醚、乙酸乙酯、正丁醇和水层萃取部位对人肝癌细胞株HepG2、人肺癌细胞
株A549、人乳腺癌细胞株MCF-7和MDA-MB-231、人宫颈癌细胞株HeLa以及人卵巢癌细胞株HO-8910的增殖抑制率，并计算其
半数抑制浓度（IC50 ）。将80只KM小鼠通过右前肢腋部皮下接种S180细胞悬液的方法复制荷瘤小鼠模型，并按体质量随机分为8
组，即模型组（生理盐水，每天灌胃2次），环磷酰胺（阳性药物）组（0.025 g/kg，每天腹腔注射给药1次），三脉菝葜醇提物高、中、低
剂量组和醇提物乙酸乙酯萃取部位高、中、低剂量组（以浸膏计给药剂量均分别为0.1、0.05、0.025 g/kg，每天灌胃2次），每组10只；
各组小鼠均连续给药14 d；末次给药后禁食不禁水12 h，检测各组小鼠的抑瘤率、脾指数和胸腺指数。结果：体外试验表明，三脉
菝葜及其不同极性萃取部位对6种肿瘤细胞的增殖抑制作用均呈现明显的量-效关系。其中以醇提物乙酸乙酯萃取部位的作用最
强，其对各肿瘤细胞的 IC50在 40～210 μg/mL 范围内，对 MCF-7 细胞、MDA-MB-231 细胞和 HeLa 细胞的 IC50分别为 70.56、83.58、
44.67 μg/mL。体内实验表明，各给药组小鼠的瘤质量较模型组均显著降低（P＜0.05或P＜0.01），并且三脉菝葜醇提物高剂量组
和醇提物乙酸乙酯部位萃取部位高、中剂量组小鼠瘤质量显著低于环磷酰胺组（P＜0.01）；环磷酰胺组、三脉菝葜醇提物高剂量组
和醇提物乙酸乙酯萃取部位高、中剂量组小鼠脾指数较模型组显著降低（P＜0.01），环磷酰胺组小鼠胸腺指数较模型组和其余各
给药组均显著升高（P＜0.01）。结论：在4个极性部位中，三脉菝葜醇提物乙酸乙酯萃取部位的抗肿瘤效果最佳，并且免疫抑制作
用较小。
关键词 三脉菝葜；醇提物；不同部位；抗肿瘤；小鼠

Study on Anti-tumor Effect of Ethanol Extract of Smilax trinervula and Its Different Polar Extract Parts
LU Yu ，YU Yingcai ，LIANG Yonghong（1.Key Laboratory of Modern Preparation of TCM，Ministry of
2
1
1，2
Education/State Key Laboratory of Efficient Energy-saving and Consumption-reducing Pharmaceutical
Equipment， Jiangxi University of TCM， Nanchang 330004， China；2.Biochemistry Laboratory of Jiangxi
University of TCM，Nanchang 330004，China）

ABSTRACT OBJECTIVE：To study the anti-tumor effect of ethanol extract of Smilax trinervula and its different polar extract
parts，and to provide reference for the screening of anti-tumor active parts. METHODS：MTT method was used to detect the
inhibitory rates of different concentrations of ethanol extract of S. trinervula，its petroleum ether，ethyl acetate，n-butanol and water
layer extract parts to the proliferation of human hepatocellular carcinoma cell line HepG2，human lung cancer cell line A549，
human breast cancer cell line MCF-7 and MDA-MB-231，human cervical cancer cell line HeLa and human ovarian cancer cell line
HO-8910. Semi-inhibitory concentration（IC50 ）was calculated. A total of 80 KM mice were subcutaneously inoculated with S180
cell suspension in right forelimb axilla to induce tumor-bearing mice model. The mice were randomly divided into 8 groups：e.g.
model group（normal saline，twice a day，i.g.），cyclophosphamide（positive drug）group（0.025 g/kg，once a day，i.p.），S.
trinervula ethanol extract high-dose， medium-dose and low-dose groups， ethyl acetate part of ethanol extract high-dose，
medium-dose and low-dose groups（0.1，0.05，0.025 g/kg by extract，twice a day，i.g.），with 10 rats in each group. The mice in
each group were given medicine for consecutive 14 days. The inhibition rate，spleen index and thymus index of mice in each group
were measured after fasting for 12 hours after the last administration. RESULTS：In vitro experiments showed that S. trinervula and
different polar extracts inhibited the proliferation of 6 kinds of tumor cells in significant dose-effect manner，especially ethyl acetate
part of ethanol extract. IC50 of it to tumor cells was 40-210 μg/mL，that of it to MCF-7，MDA-MB-231 and HeLa cells were
70.56，83.58，44.67 μg/mL，respectively. In vivo experiments
Δ 基金项目：国家自然科学基金资助项目（No.81560640）；江西省
showed that the tumor mass of mice were decreased
自然科学基金资助项目（No.20171BAB205089）；江西中医药大学校级
significantly in each administrations （P＜0.05 or P＜0.01），
科研项目（No. 20132R0047）
the tumor mass of mice in S. trinervula ethanol extract
＊硕士研究生。研究方向：天然药物抗肿瘤活性研究。E-mail：
luyu19930923 @163.com high-dose group， ethyl acetate part of ethanol extract
# 通信作者：讲师，博士。研究方向：中药药效物质基础研究。 high-dose and medium dose groups were significantly lower
E-mail：liangli4@126.com than cyclophosphamide group（P＜0.01）. The spleen index of

中国药房 2019年第30卷第19期 China Pharmacy 2019 Vol. 30 No. 19 ·2645 ·