Page 41 - 2019年10月第30卷第19期

光裸方格星虫纤溶酶对急性血瘀模型大鼠血液流变性异常及血

管内皮损伤的改善作用研究 Δ

黄媛恒 ，黄晓亮 ，覃斐章 ，李福森 ，庞丽君 ，李映新 （1.广西医科大学基础医学院，南宁 530021；2.广西医
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1＊
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科大学药学院，南宁 530021）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2019）19-2628-04
DOI 10.6039/j.issn.1001-0408.2019.19.08
摘 要 目的：研究光裸方格星虫纤溶酶（SNFE）对急性血瘀模型大鼠血液流变性异常及血管内皮损伤的改善作用。方法：将SD
大鼠随机分为对照组、模型组、阿司匹林组（100 mg/kg）和SNFE低、高剂量组（2 500、5 000 U/kg），每组10只，每天灌胃1次，连续
给药7 d，第6天给药后1 h，除对照组外，其余各组大鼠均皮下注射盐酸肾上腺素0.8 mg/kg，再以冰水浴复制急性血瘀模型。次日
给药后2 h腹主动脉采血，使用全自动血流变仪测定全血黏度（高、中、低切变率）、血浆黏度、红细胞比容、红细胞聚集指数、红细胞
变形指数等血液流变学指标，酶联免疫吸附法测定血浆中一氧化氮（NO）、内皮素1（ET-1）含量及其比值，苏木精-伊红（HE）染色
观察主动脉血管内皮的损伤程度。结果：与对照组比较，模型组大鼠的高、中、低切变率的全血黏度以及血浆黏度、红细胞聚集指
数、ET-1含量均显著升高，红细胞变形指数、NO含量和NO/ET-1比值均显著降低，差异均有统计学意义（P＜0.05或P＜0.01）。与
模型组比较，SNFE高剂量组大鼠的高、中、低切变率的全血黏度以及血浆黏度、红细胞比容、红细胞聚集指数、ET-1含量均显著降
低，红细胞变形指数、NO含量和NO/ET-1比值均显著增加，差异均有统计学意义（P＜0.05或P＜0.01）；SNFE低剂量组大鼠仅红细
胞变形指数和NO/ET-1比值均显著增加，ET-1含量显著降低，差异均有统计学意义（P＜0.05或P＜0.01）。血管内皮染色图显示，
与对照组比较，模型组大鼠主动脉各层结构松散无序，内膜缺损不完整，空泡增加，内皮损伤明显；各给药组大鼠内皮也均有不同
程度损伤，但损伤程度均较模型组轻。结论：SNFE对急性血瘀模型大鼠的血液流变性异常和血管内皮损伤均有改善作用。
关键词 光裸方格星虫纤溶酶；急性血瘀模型；血液流变性；血管内皮；大鼠

Improvement Effects of Fibrinolytic Enzyme from Sipunculus nudus on Hemorheology Disorder and
Vascular Endothelium Injury of Naked Acute Blood Stasis Model Rats
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HUANG Yuanheng ，HUANG Xiaoliang ，QIN Feizhang ，LI Fusen ，PANG Lijun ，LI Yingxin（1.School of
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Preclinical Medicine，Guangxi Medical University，Nanning 530021，China；2.School of Pharmacy，Guangxi
Medical University，Nanning 530021，China）
ABSTRACT OBJECTIVE：To study the improvement effects of fibrinolytic enzyme from Sipunculus nudus （SNFE） on
hemorheology disorder and vascular endothelium injury in naked acute blood stasis model rats. METHODS：SD rats were randomly
divided into control group，model group，aspirin group（100 mg/kg）and SNFE high-dose and low-dose groups（2 500，5 000
U/kg），with 10 rats in each group. They were given relevant medicine intragastrically once a day，for consecutive 7 d. One hour
after the 6th day of administration，except for control group，other groups were given adrenaline hydrochloride 0.8 mg/kg
subcutaneously，and then the acute blood stasis model was induced by ice-water bath. Blood was collected from abdominal aorta 2
h after the next day. Blood rheological parameters such as whole blood viscosity（high，medium and low shear rate），plasma
viscosity，hematocrit，erythrocyte aggregation index and erythrocyte deformability index were measured by automatic rheometer.
The contents of NO and ET-1 in plasma and their ratio were determined by ELISA，and the damaged degree of vascular
endothelium were observed by HE staining. RESULTS：Compared with control group，whole blood viscosity of high，medium and
low-shear rate，plasma viscosity，erythrocyte aggregation index and ET-1 content were increased significantly in model group，
while erythrocyte deformability index，NO content and NO/ET-1 ratio were decreased significantly，with statistical significance
（P＜0.05 or P＜0.01）. Compared with model group，whole blood viscosity of high，medium and low-shear rate，plasma viscosity，
hematocrit，erythrocyte aggregation index and ET-1 content were decreased significantly in SNFE high-dose groups. Erythrocyte
deformability index，NO content and NO/ET-1 ratio were increased significantly，with statistical significance （P＜0.05 or P＜
0.01）. In SNFE low-dose group，erythrocyte deformability index and NO/ET-1 ratio were increased significantly，while ET-1
content was decreased significantly，with statistical significance（P＜0.05 or P＜0.01）. Vascular endothelial staining showed that
Δ 基金项目：广西自然科学基金青年科学基金项目（No.2017- compared with control group，the structure of aorta layers in
GXNSFBA198151） model group was loose and disordered，the endothelial defect
＊讲师，硕士。研究方向：生化药理学。电话：0771-5358074。 was incomplete，the vacuoles increased，and the endothelial
E-mail：huangyuanheng@163.com damage was obvious. The endothelium of rats in each
# 通信作者：高级实验师，博士。研究方向：中药药理学。电话： administration group was damaged to varying degrees，but the
0771-5358272。E-mail：261772759@qq.com degree of injury was lighter than in model group.

·2628 · China Pharmacy 2019 Vol. 30 No. 19 中国药房 2019年第30卷第19期