Page 89 - 2019年9月第30卷第18期

小叶三点金提取物的抗炎作用及其机制研究 Δ

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苏华珍 ，唐秀能 ，魏江存 ，马家宝 ，陈 勇 ，梁丽清 ，江先再 ，韩 倩（1.广西中医药大学药学院，南宁
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530200；2.广西中医药大学第一附属医院药学部，南宁 530023）
中图分类号 R285 文献标志码 A 文章编号 1001-0408（2019）18-2532-05
DOI 10.6039/j.issn.1001-0408.2019.18.15
摘 要 目的：研究小叶三点金提取物的抗炎作用及其机制，为该药用植物的进一步开发应用提供实验依据。方法：采用二甲苯、
冰醋酸、角叉菜胶建立小鼠急性炎症模型，以地塞米松为阳性对照（0.005 g/kg），考察灌胃不同剂量小叶三点金提取物（50、30、15
g/kg）对二甲苯致正常小鼠和肾上腺摘除小鼠耳肿胀、冰醋酸致正常小鼠腹腔毛细血管通透性增加、角叉菜胶致正常小鼠和肾上
腺摘除小鼠足肿胀的抑制作用，并对角叉菜胶致炎模型肾上腺摘除小鼠足趾炎性部位中的丙二醛（MDA）、超氧化物歧化酶
（SOD）、一氧化氮（NO）水平进行检测；另设空白组小鼠为对照（灌胃等体积水）。结果：与空白组比较，小叶三点金提取物高、中剂
量组正常小鼠和肾上腺摘除小鼠耳肿胀度均显著降低，而耳肿胀抑制率显著升高；小叶三点金提取物各剂量组正常小鼠腹腔毛细
血管通透性均显著降低；小叶三点金提取物高、中剂量组正常小鼠和肾上腺摘除小鼠足肿胀度均显著降低，而足肿胀抑制率显著
升高（P＜0.05或P＜0.01）；小叶三点金提取物高、中剂量组肾上腺摘除小鼠足趾炎性部位中MDA、NO水平均显著降低，SOD水平
均显著升高（P＜0.05）。结论：小叶三点金提取物对小鼠急性炎症有明显抑制作用；其抗炎作用机制与降低MDA、NO水平并提高
SOD水平有关，且该抗炎作用的发挥不依赖于下丘脑-垂体-肾上腺轴系统。
关键词 小叶三点金；提取物；抗炎；作用机制；下丘脑-垂体-肾上腺轴系统；小鼠
Study on Anti-inflammatory Effect and Its Mechanism of the Extract of Dcsmodium microphyllum
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SU Huazhen ，TANG Xiuneng ，WEI Jiangcun ，MA Jiabao ，CHEN Yong ，LIANG Liqing ，JIANG Xianzai ，
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HAN Qian （1. College of Pharmacy，Guangxi University of TCM，Nanning 530200，China；2. Dept. of
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Pharmacy，the First Affiliated Hospital of Guangxi University of TCM，Nanning 530023，China）
ABSTRACT OBJECTIVE：To study the anti-inflammatory effect and its mechanism of the extract of Dcsmodium microphyllum，
so as to provide experiment reference for further study of D. microphyllum. METHODS：Acute inflammatory model was established
by xylene，glacial acetic acid and carrageenan. Using dexamethasone as positive control （0.005 g/kg），inhibitory effects of
intragastric different doses of the extract of D. microphyllum（50，30，15 g/kg）on xylene-induced ear swelling in normal mice and
adrenalectomized mice，glacial acetic acid-induced permeability increasing of abdominal capillaries in normal mice，carrageenan-
induced paw swelling in normal mice and adrenalectomized mice were investigated. The levels of MDA，SOD and NO in the
inflammatory tissue of toes of adrenalectomized mice were detected in carrageenan-induced inflammation model. Blank group was
set for control（ig. equal volumn of water）. RESULTS：Compared with blank group，ear swelling degree of normal mice and
adrenalectomized mice were decreased significantly in D. microphyllum extract high-dose and medium-dose groups while inhibitory
rate of ear swelling was increased significantly；the permeability of abdominal capillaries of normal mice was significantly
decreased in D. microphyllum extract groups；the swelling degree of toes in normal mice of D. microphyllum extract high-dose and
middle-dose groups and adrenalectomized mice were significantly decreased while inhibitory rate of toe swelling was increased
significantly （P＜0.05 or P＜0.01）. The levels of MDA and NO in the toe inflammatory site of adrenalectomized mice were
decreased significantly in D. microphyllum extract high-dose and medium-dose groups，while the level of SOD was increased
significantly （P＜0.05）. CONCLUSIONS：D. microphyllum extract can inhibit acute inflammation in mice significantly. Its
anti-inflammatory mechanism is associated with decreasing MDA and NO while increasing SOD levels，and the anti-inflammatory
effect does not depend on the hypothalamus-pituitary-adrenal axis system.
KEYWORDS Dcsmodium microphyllum；Extract；Anti-inflammation；Mechanism；Hypothalamus-pituitary-adrenal axis system；
Mice
Δ 基金项目：国家自然科学基金资助项目（No.81660701）；广西自
然科学基金资助项目（No.2016GXNSFAA380148）；国家中医药管理局
中医药科研实验室（三级）-中（壮）药化学与质量分析实验室（No.国中 小叶三点金为豆科山蚂蝗属植物小叶三点金草
医药发〔2009〕21号-TCM-2009-299）；广西高校中青年教师科研基础能 [Dcsmodium microphyllum（Thunb.）DC.]，别名“斑鸠窝”
力提升项目（No.2019KY0341） “爬地香”“哮灵草”“小叶山绿豆”“辫子草”“碎米柴”等，
＊主管药师。研究方向：中药药效机制。E-mail：su20503@126.
以其根及全草入药 [1-2] 。小叶三点金在民间常用于治疗
com
尿路感染、糖尿病、泌尿系结石、慢性胃炎、慢性气管炎
# 通信作者：主管药师，硕士。研究方向：中药质量分析及药效机
制。E-mail：18776728630@163.com 等病症，具有清热解毒、健脾利湿、消炎止血、止咳平喘、

·2532 · China Pharmacy 2019 Vol. 30 No. 18 中国药房 2019年第30卷第18期