Page 38 - 2019年9月第30卷第18期

Effects of Zhuangtongyin on Blood Vessels Diastolic and Systolic Function and Cardiac Function in Rats
with Coronary Heart Disease
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ZHANG Shitian ，PANG Lulu ，TANG Hanqing ，HUANG Xiaoshan ，FENG Yue ，HUANG Cenhan（1. School of
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Basic Medicine，Youjiang Medical University for Nationalities，Guangxi Baise 533000，China；2. School of
Basic Medicine，Guangxi University of TCM，Nanning 530001，China）
ABSTRACT OBJECTIVE：To observe the effects of Zhuangtongyin（ZTY）water extract on blood vessels diastolic and systolic
function and cardiac function in rats with coronary heart disease（CHD）and its potential mechanism. METHODS：SD rats were
randomly divided into control group，sham operation group，model group，positive group（Compound danshen dripping pills，0.08
g/kg）and ZTY water extract low-dose，medium-dose and high-dose groups（6.8，13.6，27.2 g/kg，by weight of water extract），
with 10 rats in each group. Except for control group（no operation）and sham operation group（only open chest without ligation），
CHD model were set up in other groups by ligating the left ventricle cyclotron endings. One week after modeling，control group，
sham operation group and model group were given constant volume of water intragastrically，and administration groups were given
relevant medicine intragastrically；once a day，for consecutive 4 weeks. Two hours after last administration，cardiac function
indexes（LVEDV，LVESV，SV，LVEF）of rats were detected in each group. The levels of inflammatory factors（CRP，IL-1β，
IFN-γ and ET-1） in myocardial tissue were determined by ELISA. Histomorphological characteristics of myocardial tissue were
determined by HE staining. RESULTS：The structure of myocardial fibers in control group and sham operation group was clear and
orderly；there was no statistical significance in difference of each index between 2 groups （P＞0.05）. Compared with control
group，myocardial fibers were disorderly arranged in model group，and the phenomena of rupture，dissolution and necrosis were
observed，accompanied by infiltration of inflammatory cells. The levels of LVEDV and LVESV as well as the levels of CRP，IL-1β，
ET-1 and IFN-γ were increased significantly，while the levels of SV and LVEF were decreased significantly（P＜0.05 or P＜0.01）.
Compared with model group，above symptoms of rats in each administration group were improved to different extents；the levels
of LVEDV and LVESV as well as the levels of CRP，IL-1β，ET-1 and IFN-γ in positive group，the levels of LVEDV in ZTY water
extract medium-dose and high-dose groups，the levels of LVESV and CRP，IL-1β，ET-1，IFN-γ in ZTY water extract groups were
decreased significantly. The levels of SV and LVEF in positive group，the levels of SV in ZTY water extract groups as well as the
levels of LVEF in ZTY water extract medium-dose and high-dose groups were increased significantly （P＜0.05 or P＜0.01）.
CONCLUSIONS：ZTY water extract could reduce the level of ET-1 and improve blood vessels diastolic and systolic function in
CHD model rats，then restore blood supply of myocardial tissue and strengthen cardiac function，by acting on inflammation cycle.
KEYWORDS Zhuangtongyin；Water extract；Coronary heart disease；Cardiac function；Blood vessels diastolic and systolic
function；Inflammatory factor；ET-1；Rat

冠心病（Coronary heart disease，CHD）是由于心肌纤 1 材料
维缺血缺氧致心泵功能受损的疾病，需要恢复血液供应 1.1 仪器
以维持正常的能量和物质代谢。内皮素1（ET-1）作为血 V-100 型小动物呼吸机（上海玉研科学仪器有限公
管舒缩功能相关因子，具有很强的促血管平滑肌收缩作 司）；Vivid 7 型彩色多普勒超声诊断仪（美国 GE 公司）；
用 [1-2] 。当CHD发生时，机体ET-1水平升高，血管发生强 AD340 型酶标仪（美国 Beckman Coulter 公司）；077526
烈收缩，使得心脏微细血管舒缩功能紊乱、心肌缺血加 型 心 电 图 机（上 海 光 电 医 用 电 子 仪 器 有 限 公 司）；
剧，是CHD进展和心力衰竭加重的重要病理环节 。近 RM2245 型切片机（上海莱卡显微系统有限公司）；
[3]
年来研究表明，C反应蛋白（CRP）、白细胞介素1β（IL-1β） AE160型电子天平（瑞士Mettler-Toledo公司）；TEC2602
以及干扰素γ（IFN-γ）等炎症因子均可影响ET-1的表达， 型恒温烘片机（意大利 Histo-Line 公司）；SM-250D 型超
为CHD治疗药物的研发提供了新的候选分子靶点 [4-6] 。 声波匀浆仪（南京舜玛仪器设备有限公司）；CK41-32PH
壮通饮是广西壮族医药的经验方，由扶芳藤、参三 型显微镜（日本Olympus公司）。
七、黄花倒水莲等3味药材组成，具有活血化瘀、通经活 1.2 药材
[7]
[8]
络的功效，可用于心血管疾病 、血栓脑梗 、高脂血症 [9] 扶芳藤饮片（广西仁济堂中药饮片有限公司，批号：
等症的治疗，疗效较好，但关于其活血化瘀的分子生物 151101）、参三七饮片（江西樟树天齐堂有限公司，批号：
学机制研究不多。为此，本研究通过建立 CHD 动物模 20131023）、黄花倒水莲饮片（广西张益堂中药饮片有限
型，以壮通饮为干预药物，观察该方对模型动物血管心 公司，批号：1602092）经右江民族医学院基础医学院李
功能以及舒缩功能相关因子ET-1、炎症因子（CRP、IL-1β、 克明副教授鉴定分别为卫矛科植物扶芳藤[Euongmus
IFN-γ）表达的影响，初步探讨其治疗 CHD 的可能机制， fortunei（Turez.）Hand.-Mazz.]的茎叶、五加科植物参三七
旨在为该症的临床治疗寻找新的靶点。 [Panax notoginseng（Burk）F. H. Chen]的干燥根、远志科

中国药房 2019年第30卷第18期 China Pharmacy 2019 Vol. 30 No. 18 ·2481 ·