Page 55 - 2019年8月第30卷第16期

响应面法结合信息熵理论优化桃核承气汤水提取工艺 Δ

1＊
顿佳颖 ，郑 鹏 ，李佳佳 ，马 遥 ，李春花 （1.河北中医学院药学院药剂学教研室，石家庄 050090；2.石
1，3 #
2
1
1
家庄市第四医院药剂科，石家庄 050011；3.河北省高校中药组分制剂应用技术研发中心，石家庄 050090）
中图分类号 R284.2 文献标志码 A 文章编号 1001-0408（2019）16-2210-06
DOI 10.6039/j.issn.1001-0408.2019.16.10

摘 要 目的：优化经典名方桃核承气汤的水提取工艺。方法：在单因素考察的基础上，结合响应面法与信息熵理论，对浸泡时
间、料液比、提取总时间进行考察，以桃核承气汤中苦杏仁苷、大黄酸、桂皮醛、甘草酸的含量为评价指标，采用信息熵理论对各评
价指标赋予权重系数，并计算综合评分。通过Design-Expert 10软件分析各因素间的交互作用，得出优化的水提取工艺，并对该优
化工艺进行验证试验。结果：根据信息熵理论将大黄酸、苦杏仁苷、甘草酸、桂皮醛的权重系数确定为 0.097 6、0.363 2、0.173 5、
0.365 7；交互作用分析结果显示，料液比对综合评分影响较大。桃核承气汤水提取工艺最佳工艺条件为浸泡时间60 min、料液比
1 ∶ 10（g/mL）、提取总时间130 min（提取3次，每次分别提取65、33、32 min）；验证试验结果显示，各指标成分含量及综合评分RSD
均小于3％。结论：优化所得的水提取工艺稳定、可行，可为桃核承气汤的进一步开发利用提供基础。
关键词 桃核承气汤；响应面法；信息熵理论；综合评分；水提取工艺；工艺优化

Optimization of Water Extraction Technology of Taohe Chengqi Decoction by Response Surface
Methodology Combined with Information Entropy Theory
2
1，3
1
DUN Jiaying ，ZHENG Peng ，LI Jiajia ，MA Yao ，LI Chunhua （1. Teaching and Research Section of Pharmacy，
1
1
College of Pharmacy，Hebei University of Traditional Chinese Medicine，Shijiazhuang 050090，China；2. Dept.
of Pharmacy，Shijiazhuang Forth Hospital，Shijiazhuang 050011，China；3. Hebei Higher Education Institute
Applied Technology Research Center on TCM Formula Preparation，Shijiazhuang 050090，China）
ABSTRACT OBJECTIVE：To optimize the water extraction technology of classic formula Taohe chengqi decoction. METHODS：
Based on single factor test，combined with response surface methodology and information entropy theory，the soaking time，
solid-liquid ratio and extraction time were investigated. Using the contents of rhein，amygdalin，cinnamaldehyde and glycyrrhizic
acid in Taohe chengqi decoction as indexes，information entropy theory was used to assign weight coefficients to each evaluation
index and calculate the comprehensive score. Through Design-Expert 10 software，the interactions of each factor were analyzed.
Water extraction technology was optimized，and validation test was also performed. RESULTS：According to information entropy
theory，the weight coefficients of rhein，amygdalin，glycyrrhizic acid and cinnamaldehyde were located at 0.097 6，0.363 2，0.173 5
and 0.365 7. The results of interaction analysis showed that the material-liquid ratio had a greater impact on the comprehensive
score. The optimal water extraction technology of Taohe chengqi decoction were determined as that soaking time was 60 min；the
ratio of material to liquid was 1 ∶ 10（g/mL）；total extraction time was 130 min（extracting for 3 times，lasting for 65，33，32 min
each time）. The results of verification test showed that RSD of content of each index component and the comprehensive score was
less than 3％. CONCLUSIONS：The optimal water extraction technology is proved to be stable and feasible，which can provide the
basis for the further development and utilization of Taohe chengqi decoction.
KEYWORDS Taohe chengqi decoction；Response surface methodology；Information entropy theory；Comprehensive score；
Water extraction technology；Technology optimization

桃核承气汤原方出自张仲景《伤寒论》辨太阳病脉 脉，芒硝泻热软坚，共为臣药；炙甘草护胃安中为佐，并
证并治太阳病证篇，其由桃仁、大黄、桂枝、芒硝、炙甘草 缓诸药之峻烈 。桃核承气汤组方精简，临床上应用广
[2]
[1]
5味药材组成，主要功用为泻热逐瘀，主治下焦蓄血证 。 泛，在国家中医药管理局发布的《古代经典名方目录（第
方中桃仁与大黄合用，瘀热并治共为君药；桂枝温通经 一批）》中位于100首经典方剂之首 。现代医药学研究
[3]
Δ 基金项目：河北省重点研发计划项目（No.19272502D）；河北省高等 表明，该方多用于治疗急性脑出血、急性脑卒中、脑梗死
学校科学技术研究项目（No.ZD2017245） 急性期、多发性脑梗塞、脑出血术后并发症、糖尿病并发
＊硕士研究生。研究方向：中药制剂新剂型研发。电话：0311-
脑梗死、肝性脑病、高脂血症、高半胱氨酸血症等；其原
89926753。E-mail：15231997020@163.com
方或加减方亦可用于治疗慢性肺源性心脏病、老年痴
# 通信作者：教授，硕士生导师，硕士。研究方向：中药制剂新剂
型研发。电话：0311-89926753。E-mail：13803369966@163.com 呆、精神分裂症、焦虑症、阿尔茨海默病等由瘀热互结所

·2210 · China Pharmacy 2019 Vol. 30 No. 16 中国药房 2019年第30卷第16期