Page 78 - 2019年1月第30卷第2期

Improvement Effects of Water Extract of Glycyrrhiza uralensis on Acute Hepatic Injury Caused by
Triptolide and Its Effects on the Levels of IL-10 and TNF-α in Rats
ZHU Shengnan ，ZHANG Jing ，TAN Qinyou 1，2，3 ，HU Qian ，SU Huazhen ，HUANG Shaoyuan ，LIU Fang（1.
1，2
1
1
1
1
1
Clinical Pharmacy & Pharmacology Research Institute，the Affiliated Hospital of Guilin Medical University，
Guangxi Guilin 541001，China；2. Laboratory of Hepatobiliary Surgery，the Affiliated Hospital of Guilin
Medical University，Guangxi Guilin 541001，China；3. China-USA Lipids in Health and Disease Research
Center，Guangxi Guilin 541001，China）
ABSTRACT OBJECTIVE：To observe the improvement effects of water extract of Glycyrrhiza uralensis on acute hepatic injury
caused by triptolide and its effects on the levels of IL-10 and TNF-α in rats. METHODS：A total of 60 healthy male SD rats were
randomized into blank control group，model group，positive control group（magnesium isoglycyrrhizinate）and G. uralensis water
extract low-dose，middle-dose and high-dose groups. Blank control group and model group were given isochoric normal saline
intragastrically；positive control group was given Magnesium isoglycyrrhizinate liquid intragastrically（13.5 mg/kg）；G. uralensis
water extract low-dose，middle-dose and high-dose groups were given G. uralensis water extract intragastrically 1 mL（120，240，
480 mg/kg，by the amount of extract），qd，for consecutive 7 d. At 8th day of administration，model group，positive control group
and G. uralensis water extract groups were given Triptolide liquid intragastrically（0.6 mg/kg）to establish acute liver injury model.
HE staining was used to detect the pathological changes of liver tissue in rats. The activities of ALT and AST in serum were
determined by enzyme coupling reaction. The protein expression of IL-10 and TNF-α in liver tissue were analyzed by Western
blotting. RESULTS：Compared with blank control group，obvious pathological changes of liver tissue in rats were observed in
model group；serum activities of ALT and AST，the protein expression level of TNF-α in liver tissue were increased significantly，
while the protein expression level of IL-10 was decreased significantly （P＜0.01）. Compared with model group，pathological
changes of liver tissue were relieved significantly. Except for the activity of AST in serum of rats in G. uralensis water extract
low-dose group，the activities of ALT and AST in serum，the protein expression level of TNF-α in liver tissue were decreased
significantly in G. uralensis groups，while the protein expression level of IL-10 in liver tissue was increased significantly（P＜0.05
or P＜0.01）. CONCLUSIONS：G. uralensis water extract can relieve triptolide-induced acute liver injury in rats，the mechanism of
which may be associated with expression down-regulation of pro-inflammatory factor TNF-α and expression up-regulation of
anti-inflammatory factor IL-10.
KEYWORDS Glycyrrhiza uralensis water extract；Triptolide；Rats；Acute liver injury；IL-10；TNF-α

甘草（Glycyrrhiza uralensis Fisch.）为豆科甘草属多 1 材料
年生草本，其药用部位是根及根茎 。中医上称为“国 1.1 仪器
[1]
老”的甘草，因其具有“能补、能和、能缓”的药性，在临床 Discovery专用型十万分之一分析天平（美国奥豪斯
上多用于缓和药性及降低药物毒性，其应用极其广泛，无 仪器上海有限公司）；Mini Protean Tetra 小型垂直电泳
论在时方还是经方中皆有出现 。已有文献和长期临床 仪、Gel Doc 2000 型凝胶成像仪、Chemi Doc XRS+型化
[1]
实践表明，甘草联用其他药物后，可能会改变药物的代 学发光成像分析仪（美国 Bio-Rad 公司）；5424R 型台式
谢，从而影响药物药效并中和药物毒性 。雷公藤甲素 高速冷冻离心机（德国 Eppendorf 公司）；IX73 型倒置显
[2]
是从卫茅科植物雷公藤（Tripteryginum wilfordii Hook. f.） 微镜（日本 Olympus 公司）；Nanodrop-2000 型超微量核
中提取所得的活性成分，具有显著的免疫抑制、抗炎、抗 酸蛋白定量仪（美国Thermo Fisher Scientific公司）。
[3]
肿瘤和抗生育等作用 ；同时，雷公藤甲素也是雷公藤的 1.2 药品与试剂
主要毒性成分，其所导致的肝损伤较为常见且机制复 甘草水提物（西安瑞鸿生物技术有限公司，批号：
[4]
杂 。研究表明，甘草对雷公藤类药物具有确切的减毒 20150901，甘草酸钠盐含量：11.8％）；雷公藤甲素对照品
作用 ，但是其减毒作用机制尚未明确。本文通过考察 （上海阿拉丁生化科技股份有限公司，批号：D1601041，
[5]
甘草水提物对雷公藤甲素致急性肝损伤模型大鼠的减 纯度：≥98％）；异甘草酸镁原料药（江苏正大天晴药业
毒作用及对其体内白细胞介素 10（IL-10）、肿瘤坏死因 有限公司，批号：1604081201，纯度：≥98％）；鼠β-肌动
子α（TNF-α）水平的影响，旨在进一步探讨甘草水提物对 蛋白（β-actin）单克隆抗体（中杉金桥生物技术有限公
雷公藤甲素致肝脏毒性的减毒作用机制。 司）；鼠IL-10单克隆抗体、鼠TNF-α单克隆抗体、辣根过

中国药房 2019年第30卷第2期 China Pharmacy 2019 Vol. 30 No. 2 ·217 ·