Page 120 - 《中国药房》2022年23期

佛手蒸制前后对大鼠燥性指标及血液代谢的影响
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聶沁馨 ，张秋霞 ，汪金玉 ，伍沛君 ，贺亚丽 ，林鸿彬（1.广州中医药大学中药学院，广州 510006；2.广州实
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验室药物与疫苗研究部，广州 510005）
中图分类号 R965 文献标志码 A 文章编号 1001-0408（2022）23-2926-06
DOI 10.6039/j.issn.1001-0408.2022.23.21
摘 要 目的 探究佛手蒸制前后对大鼠燥性相关指标和血液代谢的影响。方法 将 24 只 SD 大鼠随机分为空白组、生佛手组
（2.4 g/kg）、制佛手组（2.4 g/kg）、枳壳组（阳性对照，1.2 g/kg），每组6只。连续给药5周，动态观察大鼠体质量、摄食量、饮水量、尿
液量，检测大鼠血清中肾水通道蛋白3（AQP3）含量。采用液相色谱-质谱联用技术对大鼠血清样本进行代谢轮廓分析，通过主成
分分析（PCA）、正交偏最小二乘法-判别分析（OPLS-DA）等方法筛选差异代谢物，并将筛选后的差异代谢物映射到KEGG数据库
进行通路分析。结果 各组大鼠体质量均平缓上升；制佛手组大鼠的摄食量、饮水量均具有高于生佛手组和枳壳组的趋势，但差异
无统计学意义（P＞0.05）。给药第21天，与枳壳组比较，制佛手组大鼠尿液量显著升高（P＜0.05），血清AQP3含量显著降低（P＜
0.05），且均接近空白组。代谢组学研究发现，生佛手主要影响机体的脂代谢，制佛手主要影响机体的氨基酸代谢；生佛手组和制
佛手组之间的差异代谢物涉及半胱氨酸和蛋氨酸代谢，甘氨酸、丝氨酸和苏氨酸代谢，牛磺酸和次牛磺酸代谢等通路。结论 制佛
手可能通过影响环磷酸腺苷-蛋白激酶A通路缓和生佛手的燥性，血清AQP3含量可作为评价佛手炮制前后燥性变化的指标；生佛
手和制佛手对大鼠血清内源性代谢物的影响存在较大差异，尤其在脂质代谢方面。
关键词 佛手；蒸制；燥性；差异代谢物；代谢组学

Effects of Citrus medica before and after being steamed on dryness indexes and blood metabolism in rats
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NIE Qinxin ，ZHANG Qiuxia ，WANG Jinyu ，WU Peijun ，HE Yali ，LIN Hongbin（1. School of Traditional
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Chinese Medicine，Guangzhou University of Chinese Medicine，Guangzhou 510006， China；2. Drug and Vaccine
R&D Department， Guangzhou Laboratory， Guangzhou 510005， China）
ABSTRACT OBJECTIVE To explore the effects of Citrus medica before and after being steamed on blood metabolism and
dryness indexes in rats. METHODS Twenty-four SD rats were randomly divided into blank group， C. medica group （2.4 g/kg），
processed C. medica group （2.4 g/kg）， Citrus aurantium group （positive control， 1.2 g/kg）， with 6 rats in each group. After 5
weeks of continuous administration， the body weight， food intake， water intake and urine volume were observed dynamically. The
content of aquaporin 3 （AQP3） in serum was detected. The metabolic profile of rat serum samples was analyzed by liquid
chromatography-mass spectrometry. Principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis
（OPLS-DA） were used to screen differential metabolites， and screened differential metabolites were mapped to the KEGG database
for pathway analysis. RESULTS The body weight of rats in each group increased slowly； the food intake and water intake of
processed C. medica group tended to be higher than those in C. medica group and C. aurantium group， but there was no significant
difference among those groups （P＞0.05）. On the 21st day of administration， compared with C. aurantium group， the urine volume
of processed C. medica group was significantly increased （P＜0.05）， the serum AQP3 content of processed C. medica group was
significantly decreased （P＜0.05）， and were close to those of blank group. According to the metabonomic study， C. medica mainly
affected the lipids metabolism， and processed C. medica mainly affected the amino acid metabolism. The differential metabolites
pathway between C. medica and processed C. medica involved the metabolism of cysteine and methionine， metabolism of glycine，
serine and threonine， and metabolism of taurine and hypotaurine. CONCLUSIONS Processed C. medica might relieve the dryness
of C. medica by affecting adenosine phosphate-protein kinase
Δ 基金项目 广东省重点领域研发计划（No.2020B1111120002）；
A. The content of AQP3 in serum can be used as the
广东省岭南中药材保护资金资助项目（No.粤中医办函〔2020〕130号）；
广东省中药炮制技术传承基地建设项目（No. 粤中医办函〔2022〕52 evaluation index for the dryness of C. medica before and after
号）；大学生创新训练计划项目（No.S202110572117） processing. The effects of C. medica and processed C. medica
＊第一作者 硕士研究生。研究方向：中药炮制机理。E-mail： on endogenous metabolites in rat serum are quite different，
435816144@qq.com
especially in the aspect of lipid metabolism.
# 通信作者 副教授，硕士生导师，博士。研究方向：中药炮制机理
和饮片质量控制。电话：020-39358175。E-mail：wangjinyu@gzucm. KEYWORDS Citrus medica； steaming； dryness； differential
edu.cn metabolites； metabonomics

· 2926 · China Pharmacy 2022 Vol. 33 No. 23 中国药房 2022年第33卷第23期