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滇黄芩总黄酮不同极性萃取部位对非酒精性脂肪肝模型大鼠的
改善作用研究 Δ
王蒙蒙 ,乔 雪,方琼莲,付胜男,李欣坪,黄 丰,林玉萍(云南中医药大学中药学院暨云南省南药可持续利
#
*
用重点实验室,昆明 650500)
中图分类号 R965 文献标志码 A 文章编号 1001-0408(2022)11-1338-05
DOI 10.6039/j.issn.1001-0408.2022.11.10
摘 要 目的 研究滇黄芩总黄酮不同极性萃取部位对非酒精性脂肪肝(NAFLD)模型大鼠的改善作用。方法 采用乙醇回流提取
法制备滇黄芩总黄酮(SAF);将SAF用水分散后依次用乙酸乙酯、正丁醇进行萃取,分别得到滇黄芩总黄酮乙酸乙酯、正丁醇萃取
部位(分别记为SAFA、SAFB)。将36只大鼠分为正常组(n=6)和造模组(n=30)。造模组大鼠通过饲以高脂饲料复制NAFLD模
型。成模后,将造模组大鼠随机分为模型组(生理盐水)、非诺贝特组(阳性对照,20 mg/kg)、SAF 组(300 mg/kg)、SAFA 组(300
mg/kg)和SAFB组(300 mg/kg),每日灌胃1次,连续6周。末次灌胃后,计算大鼠的肝脏指数,检测大鼠血清中总胆固醇(TC)、三
酰甘油(TG)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平和
肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、白细胞介素1β(IL-1β)、IL-6、肿瘤坏死因子α
(TNF-α)水平,并观察大鼠肝组织病理形态学变化。结果 与正常组比较,模型组大鼠肝脏指数和血清/肝组织中 TC、TG、AST、
ALT、LDL-C、MDA、IL-1β、IL-6、TNF-α水平均显著升高(P<0.05),而HDL-C、SOD、GSH-Px水平均显著降低(P<0.05)。与模型
组比较,除SAFA组大鼠血清中HDL-C、ALT水平差异无统计学意义外(P>0.05),滇黄芩总黄酮各极性萃取部位给药组大鼠血清/
肝组织中上述指标均显著改善(P<0.05),肝组织炎症细胞浸润、脂肪空泡等现象明显改善。与SAF组和SAFA组比较,SAFB组
大鼠血清/肝组织中TC、TG、AST、MDA、IL-6、TNF-α水平均显著降低(P<0.05),SOD水平显著升高(P<0.05),肝组织病理形态学
变化改善更为明显。结论 滇黄芩总黄酮各极性萃取部位均可通过调控氧化应激、抑制炎症因子分泌而发挥其对NAFLD的改善
作用,且以正丁醇萃取部位的效果更好。
关键词 滇黄芩;总黄酮;极性萃取部位;非酒精性脂肪肝;保肝;氧化应激;炎症因子
Study on improvement effects of different polar parts from total flavonoids of Scutellaria amoena on
non-alcoholic fatty liver model rats
WANG Mengmeng,QIAO Xue,FANG Qionglian,FU Shengnan,LI Xinping,HUANG Feng,LIN Yuping(School
of Chinese Materia Medica & Key Laboratory of Sustainable Utilization of Southern Medicine of Yunnan
Province,Yunnan University of Chinese Medicine,Kunming 650500,China)
ABSTRACT OBJECTIVE To study the improvement effects of different polar parts from total flavonoids of Scutellaria amoena
on non-alcoholic fatty liver disease(NAFLD)model rats. METHODS The total flavonoids of S. amoena(SAF)were extracted by
reflux extraction with ethanol,suspended with water,and then extracted with ethyl acetate and n-butanol in order to obtain the
extraction parts of SAF(recorded as SAFA and SAFB respectively). Thirty-six rats were randomly divided into normal group(n=
6)and modeling group(n=30). Modeling group was given high-lipid diet to induce NAFLD model. After modeling,modeling
group was randomly divided into model group(normal saline),fenofibrate group(positive control,20 mg/kg),SAF group(300
mg/kg),SAFA group(300 mg/kg)and SAFB group(300 mg/kg);they were given relevant intragastical administration,once a
day,for consecutive 6 weeks. After last administration,the liver index was calculated;the levels of total cholesterol (TC),
triacylglycerol(TG),aspartate transaminase(AST),alanine transaminase(ALT),high-density lipoprotein cholesterol(HDL-C),
low-density lipoprotein cholesterol (LDL-C) in serum, the levels of superoxide dismutase (SOD), glutathione peroxidase
(GSH-Px),malondialdehyde(MDA),interleukin-1β(IL-1β),IL-6 and tumor necrosis factor-α(TNF-α)in liver tissue were
detected;the pathomorphological changes of liver tissue were observed. RESULTS Compared with normal group,the liver index,
the levels of TC,TG,AST,ALT,LDL-C,MDA,IL-1 β ,
Δ 基金项目:云南省科技计划项目[No.2017FF117(-022)];云南省
IL-6 and TNF-α in serum/liver tissue of model group were all
应用基础研究项目-中医联合专项面上项目(No.202001AZ070001-
increased significantly (P<0.05) , while the levels of
042);云南省教育厅科学研究基金项目(No.2022Y356)
* 硕 士 研 究 生 。 研 究 方 向 :中 药 资 源 与 开 发 。 E-mail: HDL-C,SOD and GSH-Px were all decreased significantly
1907964048@qq.com (P<0.05). Compared with model group,except there was no
# 通信作者:副教授,硕士生导师,硕士。研究方向:中药、天然药 statistical significance in the serum levels of HDL-C and ALT
物活性。E-mail:11217005@qq.com in SAFA group (P>0.05), above indexes in serum/liver
·1338 · China Pharmacy 2022 Vol. 33 No. 11 中国药房 2022年第33卷第11期