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新疆薰衣草精油抗氧化活性成分的组效关系研究                                                       Δ


        陈 萍 ，刘 兵，符继红（新疆大学化工学院，乌鲁木齐 830046）
               ＊
                               #
        中图分类号 R284.1；R285          文献标志码     A      文章编号     1001-0408（2021）12-1460-06
        DOI   10.6039/j.issn.1001-0408.2021.12.09

        摘   要   目的：探讨薰衣草精油抗氧化活性成分的组效关系。方法：采用1，1-二苯基-2-三硝基苯（DPPH）自由基清除法评价薰衣
        草精油的抗氧化活性；采用气质联用技术（GC-MS）结合保留指数对薰衣草精油成分进行定性分析；采用主成分分析、偏最小二乘
        法分析薰衣草精油化学成分与抗氧化活性的关系，以变量投影重要性（VIP）＞1筛选对DPPH自由基消除有较大贡献的成分。结
        果：3个不同品种共9批薰衣草精油的平均半抑制浓度（IC50 ）为4.82～9.88 mg/mL，法国蓝、H-701、Xinxun-4精油样品的平均IC50分
        别为 6.66～8.58、4.82～7.73、9.55～9.88 mg/mL；从中共鉴定出 40 个化学成分。PCA 分析结果显示，前二个主成分的累积方差贡
        献率为81.8％；9批样品被分为3个区域，不同品种各归为一类；法国蓝薰衣草精油的特征变量成分为峰32对应的乙酸薰衣草酯、
        峰9对应的反式-β-罗勒烯；H-701蓝薰衣草精油的特征变量成分为峰16对应的芳樟醇、峰30对应的乙酸芳樟酯和峰21对应的萜
        品烯-4-醇；Xinxun-4蓝薰衣草精油的特征变量成分为峰8对应的桉树脑、峰18对应的樟脑和峰20对应的2-茨醇。薰衣草精油中对
        抗氧化活性贡献较大的成分为峰16对应的芳樟醇（VIP值为2.940 0）、峰21对应的萜品烯-4-醇（VIP值为2.863 1）、峰35对应的石竹烯
        （VIP值为2.570 8）、峰8对应的桉树脑（VIP值为2.115 8）。结论：薰衣草精油具有一定的抗氧化活性，H-701精油样品的抗氧化活性较
        强；峰16对应的芳樟醇可能是薰衣草精油消除DPPH自由基贡献最大的成分。
        关键词 薰衣草精油；抗氧化活性；气质联用技术；组效关系

        Study on the Composition-activity Relationship of the Antioxidant Active Component in Essential Oil of
        Lavandula angustifolia from Xinjiang
        CHEN Ping，LIU Bing，FU Jihong（School of Chemical Engineering and Technology，Xinjiang University，
        Urumqi 830046，China）


        ABSTRACT    OBJECTIVE：To investigate the composition-activity relationship of the antioxidant active component in essential
        oil of Lavandula angustifolia from Xinjiang. METHODS：Antioxidant activity of essential oil of L. angustifolia was evaluated with
        1，1-diphenyl-2-picrylhydrazyl（DPPH）free radicals scavenging method. GC-MS combined with retention index were adopted to
        qualitatively analyze the compounds. The relationship of chemical components with antioxidant activity of essential oil of
        L. angustifolia was analyzed by principal component analysis （PCA） and partial least squares analyses （PLS）. The variable
        importance in projection（VIP＞1）was used to screen the components which had great contribution to the elimination of DPPH free
        radical. RESULTS：The IC 50 values of essential oil in 9 batches of L. angustifolia from 3 different varieties was 4.82-9.88 mg/mL，IC50
        of France blue was 6.66-8.58 mg/mL，IC50 of H-701 and Xinxun-4 were 4.82-7.73 mg/mL and 9.55-9.88 mg/mL，respectively. A
        total of 40 chemical components were identified of essentil oil in 9 batches of L. angustifolia. Results of PCA analysis showed that
        accumulative variance contribution rate of former 2 main components was 81.8％；9 batches of samples were divided into 3 regions，
        and different varieties were classified into one category；the characteristic variable components of the essential oil of French blue
        samples were lavandulyl acetate corresponding to peak 32 and trans-β-ocimene corresponding to peak 9；the characteristic variable
        components of the essential oil of H-701 samples were linalool corresponding to peak 16，linalyl acetate corresponding to peak 30
        and terpinen-4-ol corresponding to peak 21；the characteristic variable components of the essential oil of Xinxun-4 samples were
        eucalyptol corresponding to peak 8，camphor corresponding to peak 18 and 2-borneol corresponding to peak 20. The compound
        with the greatest antioxidant activity was linalool corresponding to peak 16 （VIP＝2.940 0）， followed by terpenein-4-ol
        corresponding to peak 21（VIP＝2.863 1），caryophyllene corresponding to peak 35（VIP＝2.570 8）and eucalyptol corresponding
        to peak 8（VIP＝2.115 8）. CONCLUSIONS：The essential oil of L. angustifolia has certain antioxidant activity，and H-701 sample
        has higher antioxidant activity. Linalool corresponding to peak 16 is the most important component of essential oil of L. angustifolia
        in eliminating DPPH free radical.
        KEYWORDS     Essential oil of Lavandula angustifolia；Antioxidant activity；GC-MS；Composition-activity relationship

            Δ 基 金 项 目 ：新 疆 维 吾 尔 自 治 区 自 然 科 学 基 金 资 助 项 目
        （No.2020D01C032）；国家自然科学基金资助项目（No.21565024）
                                                                薰衣草Lavandula angustifolia Mill.为唇形科薰衣草
            ＊ 硕 士 研 究 生 。 研 究 方 向 ：天 然 产 物 活 性 成 分 。 E-mail：
                                                            属植物。新疆伊犁是我国薰衣草的主要种植地区，与法
        cplw490311@163.com
            # 通信作者：教授，硕士生导师，博士。研究方向：天然产物活性                  国的普罗旺斯、日本北海道的富良野同为世界三大薰衣
                                                                  [1]
        成分。E-mail：fjh.518@163.com                           草基地 。维吾尔医学认为，薰衣草能消散寒气、燥湿止

        ·1460 ·  China Pharmacy 2021 Vol. 32 No. 12                                 中国药房    2021年第32卷第12期
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