Page 88 - 《中国药房》2020年第24期

丹桃金提取物对慢性铅中毒模型小鼠肝、肾氧化应激损伤的改善

作用研究 Δ

张永昕 ，曹春芽 ，龚 明 （1.解放军南部战区空军医院药剂科，广州 510602；2.湖南医药学院药学院，湖南
1＊
2 #
2
怀化 418000）
中图分类号 R285 文献标志码 A 文章编号 1001-0408（2020）24-3026-05
DOI 10.6039/j.issn.1001-0408.2020.24.13
摘 要 目的：研究丹桃金（丹参、桃仁、郁金）提取物对慢性铅中毒模型小鼠肾、肝氧化应激损伤的改善作用。方法：将72只小鼠
随机分为正常对照组、模型组、阳性对照组（二巯基丁二酸，70 mg/kg）和丹桃金提取物低、中、高剂量组（20、40、60 g/kg），每组12
只。除正常对照组外，其余各组小鼠均腹腔注射醋酸铅溶液20 mg/kg（隔日1次，连续20 d）建立慢性铅中毒模型。建模完成后，各
给药组小鼠灌胃相应药物，正常对照组和模型组小鼠灌胃等体积水，每日1次，连续20 d。末次给药后，称定小鼠体质量，计算脏
器（肾脏、肝脏）系数，采用全自动生化检测仪检测血清中天冬氨酸转氨酶（ALT）、丙氨酸转氨酶（AST）、尿素氮（BUN）、血肌酐
（Scr）水平，苏木精-伊红染色法观察肝、肾组织病理学特征，酶联免疫吸附测定法检测肝、肾组织中谷胱甘肽过氧化物酶
（GSH-Px）、超氧化物歧化酶（SOD）、丙二醛（MDA）水平。结果：与正常对照组比较，模型组和丹桃金提取物各剂量组小鼠体质量
（高剂量组除外）和SOD（高剂量组除外）、GSH-Px水平均显著降低，肾脏系数（高剂量组除外）、肝脏系数（低、中、高剂量组除外）
和BUN（高剂量组除外）、Scr、AST（高剂量组除外）、ALT、MDA水平均显著升高（P＜0.05或P＜0.01），肾小球、肾小管上皮细胞萎
缩，肝细胞排列松散及部分细胞坏死。与模型组比较，阳性对照组和丹桃金提取物各剂量组小鼠体质量和SOD、GSH-Px水平均
显著升高，肾脏系数、肝脏系数和BUN、Scr、AST、ALT、MDA水平均显著降低（P＜0.05或P＜0.01）；且丹桃金提取物中、高剂量组
小鼠肝、肾组织病理学变化显著改善。结论：丹桃金提取物对慢性铅中毒模型小鼠肝、肾氧化应激损伤均有改善作用，其作用机制
可能与消除活性氧自由基、抑制脂质过氧化、增强抗氧化防御能力有关。
关键词 丹桃金提取物；慢性铅中毒；氧化应激；抗氧化；小鼠

Improvement Effects of Dantaojin Extract on Oxidative Stress Injury of Liver and Kidney in Chronic
Lead Poisoning Model Mice
2
1
ZHANG Yongxin ，CAO Chunya ，GONG Ming（1. Dept.of Pharmacy，Air Force Hospital of Southern Theater
2
Command PLA，Guangzhou 510602，China；2. School of Pharmacy，Hunan Medical College，Hunan Huaihua
418000，China）
ABSTRACT OBJECTIVE：To study the improvement effects of Dantaojin （Salvia miltiorrhiza，Persicae Semen，Curcumae
Radix）extract on oxidative stress of liver and kidney in chronic lead poisoning model mice. METHODS：Totally 72 mice were
randomly divided into normal control group，model group，positive control group（dimercaptosuccinate，70 mg/kg），Dantaojin
extract low-dose，medium-dose and high-dose groups（20，40，60 g/kg），with 12 mice in each group. Except for normal control
group，other groups were given intraperitoneal injection of lead acetate solution 20 mg/kg（every other day，consecutive 20 days）
to establish chronic lead poisoning model. After modeling，administration groups were given relevant medicine intragastrically，
normal control group and model group were given constant volume of water intragastrically，once a day，for consecutive 20 days.
After last medication，body weight of mice was weighed，and organ coefficients（kidney and liver）were calculated. Serum levels
of ALT，AST，BUN and Scr were determined by automatic biochemical detector. HE staining was used to observe histopathological
features of liver and kidney. ELISA method was used to determine the levels of GSH-Px，SDO and MDA in liver and renal tissue.
RESULTS：Compared with normal control group，body weight （except for high-dose group），the levels of SOD （except for
high-dose group） and GSH-Px were all decreased significantly in model group and Dantaojin extract groups，while the renal
coefficients（except for high-dose group），liver coefficients（except for low-dose，medium-dose and high-dose group），the levels
of BUN（except for high-dose group），Scr，AST（except for high-dose group），ALT and MDA were increased significantly（P＜
0.05 or P＜0.01）. The epithelial cells of glomerulus and renal tubules were atrophied，the arrangement of hepatocytes was loose
and some cells were necrotic. Compared with model group，
Δ 基金项目：湖南省科技计划项目（No.2015SK20167）
body weight，the levels of SOD and GSH-Px were increased
＊主管药师，硕士。研究方向：医院药学、中药药理。电话：020-
61639532。E-mail：20691072@qq.com significantly in positive control group and Dantaojin extract
# 通信作者：副教授，硕士。研究方向：中药有效成分的提取分 groups，while the renal and liver coefficients，the levels of
离。E-mail：bdzjyyxn123@163.com BUN，Scr，AST，ALT and MDA were decreased significantly

·3026 · China Pharmacy 2020 Vol. 31 No. 24 中国药房 2020年第31卷第24期