Page 39 - 2021年15期

不同产地金钗石斛醇提物 HPLC 指纹图谱的建立及其抗炎作用

的谱效关系研究 Δ

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冯 月 ，段飞鹏 ，李一圣 ，邱书奇 （1.遵义医科大学珠海校区临床医学院，广东 珠海 519041；2.深圳市耳
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鼻咽喉研究所/深圳市龙岗区耳鼻咽喉医院耳鼻咽喉科，广东 深圳 518172）
中图分类号 R284.1 文献标志码 A 文章编号 1001-0408（2021）15-1825-06
DOI 10.6039/j.issn.1001-0408.2021.15.06

摘 要 目的：建立不同产地金钗石斛醇提物的高效液相色谱法（HPLC）指纹图谱，并进行其抗炎作用的谱效关系研究。方法：制
备12批不同产地金钗石斛（S1～S12）的醇提物样品。采用HPLC法检测，并结合《中药指纹图谱相似度评价系统（2012年版）》建
立12批金钗石斛醇提物的指纹图谱并进行共有峰指认和相似度评价。以炎症因子[白细胞介素4（IL-4）、IL-6]含量为抗炎指标，采
用酶联免疫吸附试验考察12批金钗石斛醇提物对脂多糖诱导的炎症模型RAW264.7巨噬细胞的抗炎作用；采用灰色关联度分析
法分析金钗石斛醇提物指纹图谱中各共有峰与抗炎指标的关联度。结果：12批金钗石斛醇提物共有18个共有峰，指认了12号峰
为石斛酚，各样品相似度为 0.911～0.996。金钗石斛 S1～S12 醇提物均可显著降低细胞培养液中 IL-4 的含量（P＜0.05 或 P＜
0.01），金钗石斛S1～S8、S10醇提物均可显著降低细胞培养液中IL-6的含量（P＜0.05或P＜0.01）。经灰色关联度分析发现，除4、
18号峰外，其余共有峰与IL-4含量的关联度均大于0.6，且5、7号峰与IL-4含量的关联度均大于0.8；除9、14、4、3、18号峰外，其余
共有峰与IL-6含量的关联度均大于0.6，且1、12、13号峰与IL-6含量的关联度均大于0.8。结论：12批不同产地金钗石斛醇提物的
HPLC指纹图谱的相似度较高、所含成分一致性较好；其中1、5、7、12（石斛酚）、13号峰所代表的化学成分可能是金钗石斛潜在的
抗炎药效成分。
关键词 金钗石斛；高效液相色谱；指纹图谱；抗炎作用；谱效关系
Establishment of HPLC Fingerprints of Ethanol Extract of Dendrobium nobiles from Different Habitats
and Spectrum Effect Relationship Study of Its Anti-inflammatory Effect
FENG Yue ，DUAN Feipeng ，LI Yisheng ，QIU Shuqi （1. School of Clinical Medicine，Zhuhai Campus of
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Zunyi Medical University，Guangdong Zhuhai 519041，China；2. Dept. of ENT，Shenzhen ENT Institute/
Longgang ENT Hospital，Guangdong Shenzhen 518172，China）
ABSTRACT OBJECTIVE：To establish the HPLC fingerprints of ethanol extracts of Dendrobium nobile from different habitats，
and to study spectrum effect relationship of its anti-inflammatory effect. METHODS：The ethanol extracts from 12 batches of D.
nobile from different habitats （S1-S12） were prepared. The fingerprints of ethanol extracts from 12 batches of D. nobile were
established by HPLC and TCM Fingerprint Similarity Evaluation System（version 2012）. Common peaks were identified and the
similarity was evaluated. Using the contents of inflammatory factors（IL-4，IL-6）as anti-inflammatory indexes，ELISA was used to
investigate the effects of ethanol extracts from 12 batches of D. nobile on RAW264.7 macrophages in inflammatory model induced
by lipopolysaccharide. Grey correlation analysis was used to analyze the correlation between common peaks and anti-inflammatory
indexes in the fingerprint of ethanol extract from D. nobile. RESULTS：There were 18 common peaks in ethanol extracts from 12
batches of D. nobile，and No. 12 peak was identified as dendrophenol. The similarity of each sample was 0.911-0.996. The content
of IL-4 in cell culture medium was significantly reduced by ethanol extracts of D. nobile S1-S12 （P＜0.05 or P＜0.01）；the
content of IL-6 in cell culture was significantly reduced by ethanol extracts of D. nobile S1-S8，S10（P＜0.05 or P＜0.01）. Grey
correlation analysis found that except for peaks 4 and 18，the correlation degree between the other common peaks and the content
of IL-4 was greater than 0.6，and the correlation degree between peaks 5 and 7 and the content of IL-4 was greater than 0.8；except
for peaks 9，14，4，3 and 18，the correlation degree between the other common peaks and the content of IL-6 was greater than
0.6，and the correlation degree between peaks 1，12 and 13 and the content of IL-6 was greater than 0.8. CONCLUSIONS：HPLC
fingerprints of ethanol extracts from 12 batches of D. nobile
Δ 基金项目：中国博士后科学基金资助项目（No.2020M683137）；
from different habitats have high similarity and good
深圳市龙岗区科技发展专项资金-医疗卫生科技计划项目（No.LGK-
consistency in composition； the chemical constituents
CYLWS2019000846，No.LGKCYLWS2019000749）
represented by peaks 1，5，7，12（dendrophenol）and 13 may
＊硕士研究生。研究方向：治疗变应性鼻炎的中药新药。E-mail：
769096783@qq.com be potential anti-inflammatory components of D. nobile.
# 通信作者：主任医师，教授，硕士生导师，硕士。研究方向：治疗 KEYWORDS Dendrobium nobile； HPLC； Fingerprint；
耳鼻咽喉科疾病的中药新药。 E-mail：qiuqi66858@163.com Anti-inflammatory activity；Spectrum effect relationship

中国药房 2021年第32卷第15期 China Pharmacy 2021 Vol. 32 No. 15 ·1825 ·