Page 98 - 2019年8月第30卷第16期

蒙药广枣叶总黄酮对心肌缺血再灌注损伤模型大鼠的保护作用

研究 Δ

尹 佳 ，杨若晗，赵晓东，赵海云，刘继伟，杨玉梅（内蒙古科技大学包头医学院，内蒙古 包头 014040）
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中图分类号 R541；R285 文献标志码 A 文章编号 1001-0408（2019）16-2253-05
DOI 10.6039/j.issn.1001-0408.2019.16.18

摘 要 目的：探讨广枣叶总黄酮对心肌缺血再灌注损伤（MIRI）模型大鼠的保护作用。方法：将雄性 SD 大鼠随机分为假手术
组、模型组、阳性对照组（维拉帕米，0.02 g/kg）以及广枣叶总黄酮低、高剂量组（0.1、0.4 g/kg），每组10只。各给药组大鼠灌胃相应
药物溶液（2 mL/100 g），假手术组和模型组大鼠灌胃等容生理盐水，每日 1 次，连续 7 d。末次给药后，采用改良结扎法复制大鼠
MIRI模型。采用生物机能实验系统记录再灌注期各组大鼠室性心动过速（VT）和室颤（VF）的发生次数及持续时间；采用酶联免
疫吸附双抗体夹心法检测其血清肌酐激酶（CK）活性以及肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、核因子κB（NF-κB）、一氧
化氮（NO）含量；采用苏木精-伊红染色法观察其心肌组织形态学特征；采用TTC法检测其心肌梗死范围（即梗死心肌组织质量与
心室质量的比值）。结果：与假手术组比较，模型组大鼠 VT、VF 的发生次数及持续时间均显著增加或延长，血清 CK 活性以及
TNF-α、IL-6、NF-κB含量均显著增强或升高，NO含量显著降低（P＜0.05或P＜0.01）；心肌梗死灶明显，细胞结构损坏严重，肌纤维
排列紊乱，细胞核固缩，且伴有炎症细胞浸润，其梗死心肌组织、心室的质量以及心肌梗死范围均显著增加（P＜0.01）。与模型组
比较，各给药组大鼠VT、VF的发生次数及持续时间均显著减少或缩短，血清CK活性以及TNF-α、IL-6、NF-κB含量均显著减弱或
降低，NO含量均显著升高（P＜0.05或P＜0.01）；上述心肌损伤症状均有所改善，其梗死心肌组织、心室的质量以及心肌梗死范围
均显著减少（P＜0.05或P＜0.01）。结论：广枣叶总黄酮可减轻MIRI所致的大鼠缺血性心律失常，减轻心肌组织损伤程度，减少炎
症因子释放，促进心肌和内皮细胞功能的恢复，缩小心肌梗死范围，对其具有一定的保护作用。
关键词 广枣叶；总黄酮；心肌缺血再灌注损伤；心肌酶；炎症因子；一氧化氮；大鼠

Study on Protective Effect of Total Flavonoids from the Leaves of Mongolian Medicine Choerospondias
axillaris on Myocardial Ischemia Reperfusion Injury Model Rats
YIN Jia，YANG Ruohan，ZHAO Xiaodong，ZHAO Haiyun，LIU Jiwei，YANG Yumei（Baotou Medical College，
Inner Mongolia University of Science and Technology，Inner Mongolia Baotou 014040，China）

ABSTRACT OBJECTIVE：To investigate the protective effect of total flavonoids from the leaves of Choerospondias axillaris
（TFLC）on myocardial ischemia reperfusion injury（MIRI）model rats. METHODS：Male SD rats were randomly divided into
sham operation group，model group，positive control group（verapamil，0.02 g/kg），TFLC low-dose and high-dose groups（0.1，
0.4 g/kg），with 10 rats in each group. Administration groups were intragastrically given relevant medicine（2 mL/100 g）；sham
operation group and model group were given constant volume of normal saline intragastrically，once a day，for consecutive 7 d.
After last medication，MIRI model was induced by modified ligation method. The times and duration of ventricular tachycardia
（VT）and ventricular fibrillation（VF）in rats were recorded with biological function experiment system during reperfusion period.
The activity of CK and contents of TNF-α，IL-6，NF-κB and NO in serum were determined by ELISA double antibody clip art
assay. The morphological characteristics of myocardial tissue was observed by HE staining. The myocardial infarction scope（i.e. the
ratio of myocardial tissue mass to ventricular mass）was measured by TTC method. RESULTS：Compared with sham operation
group，the times and duration of VT and VF were increased or prolonged significantly in model group；CK activity，serum
contents of TNF-α，IL-6 and NF-κB were enhanced or increased significantly，while NO content was decreased significantly（P＜
0.05 or P＜0.01）. Obvious myocardial infarction focus，serious cell structure damage，disorderly muscle fibers arrangement，cell
nucleus pyknosis and accompanied inflammatory cell infiltration were all observed in cardiac tissue；the mass of infarcted
myocardial tissue and ventricular as well as the scope of myocardial infarction increased significantly（P＜0.01）. Compared with
model group，the times and duration of VT and VF were
Δ 基 金 项 目 ：内 蒙 古 区 级 大 学 生 创 新 创 业 训 练 计 划 项 目
decreased or shortened significantly in administration groups；
（No.109-2017300405）；包头医学院“花蕾计划”项目（No.2017BYJJ-
HL-05） CK activity，serum contents of TNF-α ，IL-6，NF-κ B were
＊本科生。研究方向：心脑血管药理学与中药、蒙药药理学。 decreased significantly， while NO content was increased
E-mail：1456905405@qq.com significantly （P＜0.05 or P＜0.01）. The above symptoms of
# 通信作者：教授，硕士生导师，硕士。研究方向：心脑血管药理 myocardial injury were improved；the mass of infarcted
学与中药、蒙药药理学。电话：0472-7167795。E-mail：yym457@ali- myocardial tissue and ventricular as well as the scope of
yun.com myocardial infarction was significantly reduced （P＜0.05 or

中国药房 2019年第30卷第16期 China Pharmacy 2019 Vol. 30 No. 16 ·2253 ·