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黄芩苷对脂多糖诱导的人牙周膜干细胞增殖、迁移作用及机制
Δ

王 桥 ，冯 博 ，高永志（1.齐齐哈尔医学院附属第二医院口腔科，黑龙江 齐齐哈尔 161006；2.齐齐哈尔市
1＊
3
2
五官医院口腔科，黑龙江 齐齐哈尔 161006；3. 齐齐哈尔医学院附属第一医院口腔科，黑龙江 齐齐哈尔
161041）

中图分类号 R965 文献标志码 A 文章编号 1001-0408（2023）10-1216-07
DOI 10.6039/j.issn.1001-0408.2023.10.12

摘 要 目的 探究黄芩苷对脂多糖（LPS）诱导的人牙周膜干细胞（hPDLSCs）增殖、迁移及Janus蛋白酪氨酸激酶2（JAK2）/信号
转导和转录激活因子3（STAT3）信号通路的调控作用。方法 将hPDLSCs分为对照组、LPS组、黄芩苷不同浓度（0.1、1、10 mg/L）
组，采用ELISA法和CCK-8法测定细胞炎症因子[白细胞介素6（IL-6）、IL-1β和肿瘤坏死因子α（TNF-α）]含量和细胞活力，以筛选
最适黄芩苷浓度并进行后续通路验证实验。随后将细胞分为对照组、LPS组、最适黄芩苷浓度组、抑制剂组（10 μg/mL LPS+1 mg/L
黄芩苷＋3 μmol/L JAK2/STAT3通路抑制剂AG490），干预24 h后检测hPDLSCs增殖率、凋亡率、迁移率、侵袭细胞数、细胞周期素
D1（Cyclin D1）、胱天蛋白酶3（caspase-3）mRNA和蛋白表达及JAK2/STAT3信号通路相关蛋白表达。结果 根据细胞炎症因子含
量和细胞活力结果选择1 mg/L为黄芩苷最适浓度。与对照组比较，LPS组细胞增殖率、迁移率、侵袭细胞数、Cyclin D1 mRNA及
蛋白表达水平显著降低，细胞凋亡率、caspase-3 mRNA及蛋白表达、p-JAK2和p-STAT3蛋白表达显著升高（P＜0.05）；经1 mg/L黄
芩苷干预后，上述指标均得到显著改善（P＜0.05）；而抑制剂组上述指标的改善程度更加显著（P＜0.05）。结论 黄芩苷可通过抑
制JAK2/STAT3信号通路来促进LPS诱导的hPDLSCs增殖、迁移和侵袭，抑制其凋亡及炎症反应。
关键词 牙周炎；黄芩苷；牙周膜干细胞；Janus蛋白酪氨酸激酶2/信号转导和转录激活因子3信号通路；增殖；迁移

Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem
cells induced by lipopolysaccharide
1
WANG Qiao ，FENG Bo ，GAO Yongzhi（1. Dept. of Stomatology， the Second Affiliated Hospital of Qiqihar
2
3
Medical College， Heilongjiang Qiqihar 161006， China；2. Dept. of Stomatology， Qiqihar Wuguan Hospital，
Heilongjiang Qiqihar 161006， China；3. Dept. of Stomatology， the First Affiliated Hospital of Qiqihar Medical
College， Heilongjiang Qiqihar 161041， China）

ABSTRACT OBJECTIVE To explore the regulatory effects of baicalin on the proliferation and migration of human periodontal
ligament stem cells （hPDLSCs） induced by lipopolysaccharide （LPS） and Janus protein tyrosine kinase 2 （JAK2）/signal
transduction and transcription activator 3 （STAT3） signaling pathways. METHODS hPDLSCs were divided into control group，
LPS group， different concentration baicalin groups （0.1， 1 and 10 mg/L）. ELISA method and CCK-8 assay were used to determine
the contents of cell inflammatory factors [interleukin 6 （IL-6）， IL-1β and tumor necrosis factor-α （TNF-α）] and cell viability， so
as to screen the optimal concentration of baicalin for follow-up pathway validation experiments. The cells were then divided into
control group， LPS group， optimal baicalin concentration group and inhibitor group （10 μg/mL LPS+1 mg/L baicalin +3 μmol/L
JAK2/STAT3 pathway inhibitor AG490）. After treated for 24 h， the proliferation rate of hPDLSCs， apoptosis rate， migration rate，
invasion cell number， mRNA and protein expressions of Cyclin D1 and caspase-3， the expression of JAK2/STAT3 pathway-related
proteins were all detected. RESULTS According to cell inflammatory factors and cell viability， 1 mg/L was selected as the optimal
concentration of baicalin. Compared with control group， cell proliferation rate， migration rate， invasion cell number， Cyclin D1
mRNA and protein expression were significantly decreased in LPS group， while cell apoptosis rate， caspase-3 mRNA and protein
expression， p-JAK2 and p-STAT3 protein expression were significantly increased （P＜0.05）. After treated with 1 mg/L baicalin，
the above indexes were reversed significantly （P＜0.05）. The improvement of above indexes in the inhibitor group was more
obvious （P＜0.05）. CONCLUSIONS Baicalin can promote the proliferation， migration and invasion of LPS-induced hPDLSCs and
inhibit their apoptosis and inflammation by blocking the JAK2/STAT3 pathway.
KEYWORDS periodontitis； baicalin； periodontal ligament
Δ 基金项目 黑龙江省省属高等学校基本科研业务费科研项目
stem cells； Janus protein tyrosine kinase 2/signal transduction
（No.2021-KYYWF-0374）
＊第一作者 副主任医师。研究方向：口腔修复、口腔正畸。 and transcription activator 3 signaling pathway； proliferation；
E-mail：wangqiao090115@163.com migration

· 1216 · China Pharmacy 2023 Vol. 34 No. 10 中国药房 2023年第34卷第10期