Page 41 - 《中国药房》2021年20期

复方鳄鱼油烧伤膏对浅Ⅱ度烧伤皮肤的修复及抗炎镇痛作用 Δ

＊
#
潘 翔 ，韩思婕，陈可琢，李正磊，张丹丹，罗心遥，李慧君，夏和元，王天合，叶晓川（湖北中医药大学药学院/中
药资源与中药化学省级重点实验室，武汉 430065）
中图分类号 R285 文献标志码 A 文章编号 1001-0408（2021）20-2467-06
DOI 10.6039/j.issn.1001-0408.2021.20.06

摘 要 目的：研究复方鳄鱼油烧伤膏对浅Ⅱ度烧伤皮肤的修复及抗炎镇痛作用。方法：将加热后的砝码紧贴豚鼠右侧脱毛部位
皮肤4 s，复制浅Ⅱ度烧伤模型。造模成功后，将豚鼠按体质量随机分为模型组、京万红软膏组（阳性对照）和复方鳄鱼油烧伤膏配
方一、配方二、配方三组（鳄鱼油质量分数分别为1.5％、3％、4.5％，下同），每组8只。除模型组外，其余各组豚鼠每天按0.7 g/只涂
抹给药2次，连续14 d。每天记录豚鼠创面愈合情况，并计算创面愈合率；采用苏木精-伊红（HE）染色法观察豚鼠创面组织病理学
变化；采用酶联免疫吸附试验法测定豚鼠血清中表皮生长因子（EGF）、血管内皮生长因子（VEGF）、超氧化物歧化酶（SOD）、丙二
醛（MDA）、肿瘤坏死因子α（TNF-α）、白细胞介素1（IL-1）的水平。将80只昆明小鼠分为2组，每组再按体质量随机分为模型组、京
万红软膏组（阳性对照）和复方鳄鱼油烧伤膏配方一组、配方三组，每组10只，分别采用二甲苯耳廓肿胀法和醋酸扭体法以考察复
方鳄鱼油烧伤膏的抗炎镇痛作用。结果：在烧伤修复实验中，复方鳄鱼油烧伤膏干预后，豚鼠的创面面积逐渐缩小，且第14天时
其创面已极大幅度愈合，创面愈合率显著升高（P＜0.01）；血清中EGF、SOD水平均显著升高（P＜0.01），VEGF、MDA、TNF-α、IL-1
水平均显著降低（P＜0.05或P＜0.01）；创面组织可见较厚的新生表皮层，真皮层中结缔组织和新生血管均明显增多。在抗炎镇痛
实验中，复方鳄鱼油烧伤膏干预后，小鼠的耳肿胀度与扭体次数均显著降低（P＜0.05或P＜0.01）。结论：复方鳄鱼油烧伤膏可修
复豚鼠浅Ⅱ度烧伤皮肤，且对小鼠的抗炎镇痛作用良好；其作用机制可能与升高血清中 EGF、SOD 水平，降低 VEGF、MDA、
TNF-α、IL-1水平有关。
关键词 复方鳄鱼油烧伤膏；浅Ⅱ度烧伤；创面愈合；抗炎；镇痛
Study on Repair，Anti-inflammation and Analgesia Effects of Compound Crocodile Oil Burn Ointment on
Superficial Second-Degree Burned Skin
PAN Xiang，HAN Sijie，CHEN Kezhuo，LI Zhenglei，ZHANG Dandan，LUO Xinyao，LI Huijun，XIA Heyuan，
WANG Tianhe，YE Xiaochuan（College of Pharmacy/Key Laboratory of Chinese Medicine Resource and TCM
Chemistry，Hubei University of TCM，Wuhan 430065，China）

ABSTRACT OBJECTIVE：To study the repair，anti-inflammatory and analgesic effects of Compound crocodile oil burn ointment
on superficial second-degree burned skin. METHODS：The heated weight was attached to the right depilated skin of guinea pigs for
4 s to induce the model of superficial second-degree burn. After modeling，guinea pigs were randomly divided into model group，
Jingwanhong ointment group（positive control），formula Ⅰ，Ⅱ and Ⅲ groups of Compound crocodile oil burn ointment（volume
fraction 1.5％，3％，4.5％，hereinafter），with 8 guinea pigs in each group. Except for model group，other groups were smeared
with 0.7 g/guinea pigs twice a day for 14 consecutive days. The wound healing was recorded every day，the healing rate of wound
was calculated. HE staining was used to observe the histopathological changes of the wound. The serum levels of EGF，VEGF，
SOD，MDA，TNF-α and IL-1 were detected by ELISA. Eighty Kunming mice were divided into 2 groups，and then sub-grouped
into model group，Jingwanhong ointment group（positive control），formula Ⅰ and Ⅲ groups of Compound crocodile oil burn
ointment，with 10 mice in each group. Then xylene auricle swelling method and acetic acid writhing method were used to
investigate the anti-inflammatory and analgesic effects of Compound crocodile oil burn ointment. RESULTS：In the burn repair
experiment，after intervention of Compound crocodile oil burn ointment，the wound area of guinea pigs gradually decreased，and
on the 14th day，the wound had healed greatly，and the wound healing rate increased significantly（P＜0.01）；serum levels of
EGF and SOD were increased significantly （P＜0.01），while the levels of VEGF，MDA，TNF-α and IL-1 were decreased
significantly （P＜0.05 or P＜0.01）. The thick new epidermal layer was found in wound tissue，and the connective tissue and
neovascularization in the dermis increased significantly. In the anti-inflammatory and analgesic experiment，after intervention of
Compound crocodile oil burn ointment，the degree of ear swelling and the times of writhing decreased significantly（P＜0.05 or
P＜0.01）. CONCLUSIONS：Compound crocodile oil burn ointment shows good skin repair，anti-inflammatory and analgesic
efficacy；the mechanism may be associated with increasing the serum levels of EGF and SOD and reducing the levels of VEGF，
MDA，TNF-α，IL-1.
Δ 基金项目：湖北省技术创新专项（重大项目）（No.2019ACA121）
KEYWORDS Compound crocodile oil burn ointment；
＊硕士研究生。研究方向：中药及其制剂的物质基础。电话：
027-688890101。E-mail：1936400367@qq.com Superficial second-degree burned skin；Wound healing；Anti-
# 通信作者：教授，博士生导师，博士。研究方向：中药药效物质 inflammatory；Analgesia
基础。电话：027-688890101。E-mail：yxxcc1965@163.com

中国药房 2021年第32卷第20期 China Pharmacy 2021 Vol. 32 No. 20 ·2467 ·