Page 75 - 2020年2月第31卷第3期

不同产地丫蕊花不同部位中3种皂苷成分含量测定及差异分析 Δ

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陈 帅 ，张 美，袁崇均 ，罗 森，王 笳（四川省中医药科学院中药药学研究所，成都 610041）
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中图分类号 R917；R284.1 文献标志码 A 文章编号 1001-0408（2020）03-0325-05
DOI 10.6039/j.issn.1001-0408.2020.03.14

摘 要 目的：建立测定丫蕊花中重楼皂苷Ⅱ、Ⅵ、Ⅶ含量的方法，比较不同产地丫蕊花不同部位中这3种皂苷成分的含量差异。
方法：采用高效液相色谱法测定10个产地丫蕊花的全草部位和地下部位中重楼皂苷Ⅱ、Ⅵ、Ⅶ的含量，色谱柱为Kromasil C18，流动
相为乙腈-水（梯度洗脱），流速为1.0 mL/min，柱温为35 ℃，检测波长为203 nm，进样量为10 μL。以3种皂苷成分的含量为指标，
对20批次丫蕊花进行聚类分析和偏最小二乘判别分析，对样品进行聚类区分，并确定其主要差异性成分。结果：重楼皂苷Ⅱ、重
楼皂苷Ⅵ、重楼皂苷Ⅶ的检测质量线性范围分别为 0.051～2.04、0.007～0.28、0.168～6.72 μg（r≥0.999 5）；检测限分别为 1.92、
1.75、1.87 ng，定量限分别为 6.40、5.87、6.23 ng；精密度、重复性、稳定性（24 h）的 RSD 均小于 2％（n＝6）；平均回收率分别为
99.29％、101.38％、99.64％，RSD分别为1.17％、2.64％、0.75％（n＝6）。20批次样品的重楼皂苷Ⅱ含量为0.615～1.875 mg/g，重楼
皂苷Ⅵ含量为0～0.095 mg/g，重楼皂苷Ⅶ含量为3.158～12.354 mg/g。聚类分析结果显示，20批次样品被聚为两类，批次S9～S12
为一类，其余16批次样品被聚为一类；偏最小二乘判别分析结果显示，20批次样品分为3个区，批次S1、S2、S8、S14、S16、S20被分
为Ⅰ区，批次S9～S12被分为Ⅱ区，其余样品被分为Ⅲ区。不同产地的丫蕊花存在品质差异，且全草和地下部位中3种皂苷成分无
差异性。权重排序结果显示，重楼皂苷Ⅶ为丫蕊花药材中主要差异性成分，其中彭州和大邑县产丫蕊花药材中重楼皂苷Ⅶ最高。
结论：本方法简单方便、灵敏度高，可用于丫蕊花中3种皂苷成分的含量测定。以彭州和大邑县产丫蕊花活性成分含量较高、质量
较好。
关键词 高效液相色谱法；丫蕊花；重楼皂苷Ⅱ；重楼皂苷Ⅵ；重楼皂苷Ⅶ；聚类分析；偏最小二乘判别分析

Content Determination and Difference Analysis of 3 Saponins in Different Parts of Ypsilandra thibetica
from Different Producing Areas
CHEN Shuai，ZHANG Mei，YUAN Chongjun，LUO Sen，WANG Jia（Institute of TCM，Sichuan Academy of
TCM Sciences，Chengdu 610041，China）

ABSTRACT OBJECTIVE：To establish the method for content determination of polyphyllin Ⅱ，Ⅵ，Ⅶ in Ypsilandra thibetica，
and to compare the differences of 3 saponins in different parts of Y. thibetica from different producing areas. METHODS：HPLC
method was adopted to determine the contents of polyphyllin Ⅱ，Ⅵ，Ⅶ in whole grass part and underground part of Y. thibetica
from 10 producing areas. The determination was performed on Kromasil C18 column with mobile phase consisted of acetonirile-water
（gradient elution）at the flow rate of 1.0 mL/min. The column temperature was 35 ℃，and detection wavelength was set at 203
nm；sample size was 10 μL. With the contents of 3 saponins as the index，20 batches of Y. thibetica were analyzed by cluster
analysis and PLS-DA analysis；the aggregation of samples was analyzed and determined the primary difference components.
RESULTS：The linear range of polyphyllin Ⅱ，polyphyllin Ⅵ and polyphyllin Ⅶ were 0.051-2.04，0.007-0.28，0.168-6.72 μg，
respectively（r≥0.999 5）；the detection limits were 1.92，1.75，1.87 ng，respectively；and the quantitative limits were 6.40，5.87，
6.23 ng，respectively；RSD of precision，reproducibility and stability tests（24 h）were all lower than 2％（n＝6）；the average
recovery rates were 99.29％，101.38％ and 99.64％，with RSDs of 1.17％，2.64％，0.75％（n＝6），respectively. The content of
polyphyllin Ⅱ was 0.615-1.875 mg/g，that of polyphyllin Ⅵ was 0-0.095 mg/g，and that of polyphyllin Ⅶ was 3.158-12.354
mg/g. Cluster analysis showed that 20 batches of samples were clustered into two groups，batch S9-S12 were clustered in to one
group，and the other 16 batches of samples were clustered into another group. PLS-DA analysis showed that 20 batches of samples
were divided into 3 areas，batch S1，S2，S8，S14，S16，S20 were included in area Ⅰ；batch S9-S12 included in area Ⅱ；and the
Δ 基金项目：四川省公益性科研院所基本科研业务专项（No. rest of the samples included in area Ⅲ. The quality of Y.
A-2017N-35）；中医药公共卫生服务补贴专项“全国中药资源普查项 thibetica from different habitats was different，and there was
目”（No.财社〔2017〕66号） no difference in the saponin composition between the whole
＊副研究员。研究方向：中药化学。电话：028-85237395。E- grass and the underground part. Weight ranking found that
mail：cscdtcm@126.com polyphyllin Ⅶ was the main difference component in Y.
# 通信作者：主任中药师。研究方向：天然活性成分研究与开
thibetica，and the content of polyphyllin Ⅶ in Y. thibetica
发。E-mail：86071401@qq.com

中国药房 2020年第31卷第3期 China Pharmacy 2020 Vol. 31 No. 3 ·325 ·